We narrowed to 12,187 results for: LEA
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Plasmid#112287PurposeFluorescently labels human YAP1-2gamma isoform (504 aa) Y357F mutant _ corresponding to tyrosine 407 in this isoformDepositorInsertEYFP-YAP1 (YAP1 Human)
UseLentiviralMutationTyrosine 357 to Phenyalanine corresponding to tyr…PromoterEF-1 alphaAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
Myc-PCDHGC5
Plasmid#119935PurposeProtocadherin gammaC5 expression (Myc-tag is at the linker between EC1 and EC2 extracellular cadherin repeats)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPCDHGC5 (Pcdhgc5 Rat)
Tags9E10 cMyc epitope (EQKLISEEDL) was inserted betwe…ExpressionMammalianAvailable sinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAcGFP-Jpx9C104A
Plasmid#120380PurposeReporter for RNA nuclear localizationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertJPX (JPX Human)
TagsAcGFP1ExpressionMammalianMutationC321APromoterCMVAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR-actin-G168D,Y169D
Plasmid#118384PurposeGateway compatible donor vector with actin-G168D,Y169D.DepositorAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
Myc-delta
Plasmid#118037PurposeGABAA receptor expression (delta subunit) Myc-tag near N-terminusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGABRD (Gabrd Rat)
TagscMyc epitope EQKLISEEDL inserted between fourth a…ExpressionMammalianAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-mPC1_4077-eGFP-3HA
Plasmid#108390PurposeExpress cytoplasmic tail mouse PC1 from amino acid 4077 to 4293DepositorAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTKan-p35S::Csy4-pNOS::cogGFP (C132, JBEI-15898)
Plasmid#110145PurposeTransformation and expression of Csy4 and cogGFP proteins in plantsDepositorAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTKan-p35S::Csy4-pNOS::cogRFP (C134, JBEI-15901)
Plasmid#110146PurposeTransformation and expression of Csy4 and cogDsRed proteins in plantsDepositorAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDY-HA1-HA2-neo
Plasmid#109123PurposeFR cassette provider to introduce FR cassette to a BAC by recombineeringDepositorInsertsHA1
HA2
Available sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNIC-ZB-SPRTN-Y117C-FL
Plasmid#110219PurposeExpression in E. coli of full-length SPRTN protein carrying Y112A mutation, a catalytically impaired mutant, with His and ZB tags at N-terminus.DepositorInsertSPRTN (SPRTN Human)
TagsHis6 and ZBExpressionBacterialMutationY117C, P296L (see depositor comments below)Available sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-hPC1_MTS-eGFP
Plasmid#108402PurposeExpress putative mitochondrial targeting sequence of human PC1 from amino acid 4129 to 4157DepositorAvailable sinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28b – E. coli RecE residues 564-868
Plasmid#104534PurposeExpresses E. coli RecE residues 564-868DepositorInsertRecE residues 564-866 (C-terminal nuclease domain)
Tags6X-His tag followed by thrombin siteExpressionBacterialPromoterT7Available sinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLITMUS-rpoN-cI4A5C6G7G-J23106-geneIII
Plasmid#80909PurposePhagemid (encodes cI4A5C6G7G and M13 gene III)DepositorInsertsM13 gene III
cI4A5C6G7G
ExpressionBacterialMutation238G to 238D (please see depositor comment below)PromoterBBa_J23106 and rpoNAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
NFATc2-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)
Plasmid#75234PurposeCRISPR/Cas9 NICKASE plasmid against human NFATc2 (1/2)DepositorInsertsgRNA against NFATc2 (NFATC2 Human)
UseCRISPRAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
NFATc2-CRISPR-Nick 2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)
Plasmid#75235PurposeCRISPR/Cas9 NICKASE plasmid against human NFATc2 (2/2)DepositorInsertsgRNA against NFATc2 (NFATC2 Human)
UseCRISPRAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
PHF13 (3O7A)
Plasmid#28155PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPHD finger protein 13 (PHF13 Human)
TagsHis tag with TEV cleavageExpressionBacterialMutationPHD-type zinc finger domain (residues 250-301.Available sinceApril 4, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
RFXANK (3V3O)
Plasmid#36905PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertRFXANK (RFXANK Human)
TagsHis tag with TEV cleavageExpressionBacterialMutationamino acid residues 90-260 (ANK repeats)Available sinceJune 19, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
MLH1
Plasmid#32859PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertMLH1 (MLH1 Human)
TagsHis (with TEV cleavage)ExpressionBacterialMutationcontains amino acids 486-751Available sinceFeb. 22, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEMS1136
Plasmid#29056PurposeExpression of the eGFP reporter gene was not detected in the brain and eye.DepositorAvailable sinceNov. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEMS1408
Plasmid#29205PurposeExpression of the reporter gene under the control of the MiniPromoter in this plasmid has not been testedDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPle201 (SLC6A5 Human)
UsePleiades promoter project [sic, pleaides plieades]TagsEGFP-NLSAvailable sinceNov. 15, 2011AvailabilityAcademic Institutions and Nonprofits only