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We narrowed to 11,809 results for: emb

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Showing: 7041 - 7060 of 11809 results

  1. pGST-HsSyx-375-792

    Plasmid
    #179292
    Purpose
    E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 375-792) with N-terminal His6 + GST + TEV protease cleavage site
    Depositor
    Debra Hansen
    Publication
    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.
    Insert
    Syx
    Tags
    His6 + GST + TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    residues 375-792 from accession number NP_0010361…
    Promoter
    T7
    Available since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    179292-plasmid-map-sequence-id-354918

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  2. pelB-MBP-RevA-ss

    Plasmid
    #137064
    Purpose
    E. coli expression clone (T7lac promoter) for mature RevA (aa 25-160) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage
    Depositor
    Debra Hansen
    Publication
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAF07416.1 (revA Borrelia burgdorferi B31)
    Tags
    PelB signal peptide + His10 + maltose binding pro…
    Expression
    Bacterial
    Mutation
    lacks the RevA signal peptide and 5 residues (CKA…
    Promoter
    T7lac
    Available since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    137064-plasmid-map-sequence-id-291953

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  3. pKIF1C-2xFLAG

    Plasmid
    #130979
    Purpose
    Expression of KIF1C-2xFLAG in mammalian cells.
    Depositor
    Anne Straube
    Publication
    Theisen et al Dev Cell. 2012 Dec 11;23(6):1153-66. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    KIF1C-2xFLAG (KIF1C Human)
    Tags
    2xFLAG
    Expression
    Mammalian
    Mutation
    4 silent mutations that make this construct resis…
    Promoter
    CMV
    Available since
    Oct. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    130979-plasmid-map-sequence-id-259357

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  4. pEGFP-C1-FLAG-GFP-Ubl4A-H1(90-107)

    Plasmid
    #87202
    Purpose
    To express Ubl4A (90-107 residues) tagged with FLAG-EGFP at N- terminus.
    Depositor
    Yihong Ye
    Publication
    Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    UBL4A (UBL4A Human)
    Tags
    FLAG and GFP
    Expression
    Mammalian
    Mutation
    Residues from 90-107 are inserted.
    Promoter
    CMV
    Available since
    April 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    87202-plasmid-map-sequence-id-175665

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  5. GFP-KIF1B beta-600-1400

    Plasmid
    #79109
    Purpose
    Mammalian expression of GFP-KIF1B beta-600-1400
    Depositor
    Susanne Schlisio
    Publication
    Chen et al Cancer Discov. 2014 Apr;4(4):434-51. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GFP-KIF1B beta (600-1400 amino acids) (KIF1B Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    exon 25 missing (natural splice varient)
    Promoter
    CMV
    Available since
    June 14, 2016
    Availability
    Academic Institutions and Nonprofits only

  6. pJ145

    Plasmid
    #29491
    Depositor
    Jonathan Tait
    Publication
    Jin et al J Biol Chem 2004; 279:40351-57.
    Insert
    annexin V-128 (ANXA5 Human)
    Tags
    (Met)-Ala-Gly-Gly-Cys-Gly-His
    Expression
    Bacterial
    Mutation
    Deletion of the initiator Met at position 1 of wi…
    Available since
    June 7, 2011
    Availability
    Academic Institutions and Nonprofits only

  12. GFP Cav2.2 human HAgHA -plus exon 18a - LONG-Cterm in pcDNA3

    Plasmid
    #198451
    Purpose
    N-terminal GFP tagged, exofacial HAgHA tag in Domain II, contains exon 18a- Long form of alternatively spliced C-teminus, in pcDNA3 vector
    Depositor
    Annette Dolphin
    Publication
    Dahimene et al Function (Oxf). 2023 Oct 19;5(1):zqad060
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cav2.2 (CACNA1B Human)
    Tags
    N-terminal GFP, internal double HA
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    198451-plasmid-map-sequence-id-392120

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  13. pSems leader-ALFA-mXFP-TpoR

    Plasmid
    #222945
    Purpose
    Expression of TPOR with ALFAtag and monomeric XFP in mammalian cells
    Depositor
    Jacob Piehler
    Publication
    Winkelmann et al Nat Commun. 2024 Jul 10;15(1):5813. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Thrombopoietin receptor (26-635) (MPL Human)
    Tags
    ALFAtag, Ig k-chain leader sequence, and mXFP
    Expression
    Mammalian
    Mutation
    mXFP: tryptophan 66 to phenylalanine, TPOR: Signa…
    Promoter
    CMV
    Available since
    Jan. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    222945-plasmid-map-sequence-id-457424

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  15. pTRIP TRE BI_SEPT9_i1 NCmut-14-GFP11_SEPT9_i1 NCmut-14-GFP10

    Plasmid
    #180358
    Purpose
    mammalian co-expression of human SEPT9_i1 NCmut fused to GFP10 and of human SEPT9_i1 NCmut fused to GFP11
    Depositor
    Manos Mavrakis
    Publication
    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301
    Inserts
    SEPTIN9_v1 (SEPTIN9 Human)
    SEPTIN9_v1 (SEPTIN9 Human)
    IRES-TagBFP
    Use
    Lentiviral
    Tags
    GFP10 and GFP11
    Expression
    Mammalian
    Mutation
    SEPT9_i1 I281D, M288D
    Promoter
    bidirectional TRE
    Available since
    Dec. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    180358-plasmid-map-sequence-id-358016

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  17. pEGFP-C1-FLAG-GFP-Ubl4A-H2(108-130)

    Plasmid
    #87203
    Purpose
    To express Ubl4A (108-130 residues) tagged with FLAG-EGFP at N- terminus.
    Depositor
    Yihong Ye
    Publication
    Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    UBL4A (UBL4A Human)
    Tags
    FLAG and GFP
    Expression
    Mammalian
    Mutation
    Residues from 108-130 are inserted.
    Promoter
    CMV
    Available since
    April 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    87203-plasmid-map-sequence-id-175668

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  19. pRS405 SEC66-VAC8-GFP

    Plasmid
    #166840
    Purpose
    Expresses ER localized Vac8-GFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 162 base pairs from the SEC66 ORF, which encode the Sec66 transmembrane domain.
    Depositor
    Daniel Klionsky
    Publication
    Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554
    Insert
    VAC8 (VAC8, SEC66 Budding Yeast)
    Tags
    GFP and SEC66 transmembrane domain
    Expression
    Yeast
    Mutation
    SEC66 transmembrane domain
    Promoter
    VAC8 promoter
    Available since
    April 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    166840-plasmid-map-sequence-id-329328

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  20. pSB-CMV-MCS-Puro ArfGAP1_ALPS1_ALPS2-mKate2-3NLS-P2A-EGFP-LMNB1

    Plasmid
    #250908
    Purpose
    Dual color expression of mKate2-3NLS tagged human ARFGAP1 ALPS 1-2 and EGFP tagged zebrafish LaminB1 in mammallian cell lines
    Depositor
    Philipp Niethammer
    Publication
    Shen et al Nat Cell Biol. 2025 Dec 9. doi: 10.1038/
    Insert
    ArfGAP1-mKate2-3xNLS-P2A-EGFP-Lamin B1 (lmnb1, ARFGAP1 Zebrafish, Human)
    Tags
    EGFP on LMNB1 and mKate2-3xNLS on ArfGAP1
    Expression
    Mammalian
    Mutation
    ARFGAP1 amino acids 192–304 only
    Promoter
    CMV
    Available since
    Jan. 28, 2026
    Availability
    Academic Institutions and Nonprofits only
    250908-plasmid-map-sequence-id-498469

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Showing: 7041 - 7060 of 11809 results