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pCRI009-pKW20088-PelcA-mCherry-TtrpC-NotI-PacI

Plasmid

#140202

Purpose

CRISPRa proof-of-concept test target with fluorescent reporter. PelcA is fused to mCherry, with low basal expression in Aspergillus nidulans. Fungal vector with AMA1 and pyrG selection marker.

Depositor

Yit Heng Chooi

Article

Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

Insert

mCherry

Use

CRISPR and Synthetic Biology; Proof-of-concept te…

Mutation

G174D

Promoter

PelcA

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pPW3759 : CMVd3-HsIRE1deltaLD-HaloTag

Plasmid

#185681

Purpose

Low-level transient expression of delta-lumenal domain HsIRE1 with a C-terminal HaloTag.

Depositor

Peter Walter

Article

Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

Insert

ERN1 (ERN1 Synthetic, Human)

Tags

HaloTag

Expression

Mammalian

Mutation

Deletion of lumenal domain

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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CSII-IDR2-owlTRIMCyp-FOS

Plasmid

#79067

Purpose

Express owl monkey TRIMCyp in mammalan cells

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

TRIM5Cyp (owl monkey)-FOS

Tags

OneSTrEP-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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PDF1::mCitrine-MBD

Plasmid

#115222

Purpose

Microtubule visualization

Depositor

Jan Traas

Article

Armezzani et al Development. 2018 Jun 4;145(11). pii: de

Insert

Microtubule binding domain

Tags

mCitrine

Expression

Plant

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pT/Caggs-NRAS G12V-IRES-ASK1

Plasmid

#221074

Purpose

Expresses NRAS G12V and ASK1 in mammalian cells

Depositor

Hidenori Ichijo

Article

Odawara et al Commun Biol. 2024 Jun 5;7(1):691. doi: 1

Inserts

NRAS (NRAS Human)
ASK1

Expression

Mammalian

Mutation

G12V

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pLB62_PB_pGK-H2B-mIFP-T2A-rTetR-humanKRAB-zeo

Plasmid

#179439

Purpose

Expresses rTetR-KRAB(human) for gene silencing, integration by Piggybac

Depositor

Lacra Bintu

Article

Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

Insert

H2B-mIFP-T2A-rTetR-KRAB

Expression

Mammalian

Promoter

pGK

Available Since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEBB COMMD4 Flag

Plasmid

#74896

Purpose

Express COMMD4 in mammalian cells

Depositor

Ezra Burstein, Colin Duckett

Article

Burstein et al J Biol Chem. 2005 Jun 10;280(23):22222-3

Insert

COMMD4 (COMMD4 Human)

Tags

Flag

Expression

Mammalian

Promoter

EF-1alpha promoter

Available Since

June 23, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA-HA-hTRAF3 333-C

Plasmid

#44035

Depositor

Shao-Cong Sun

Article

Liao et al J Biol Chem. 2004 Jun 18;279(25):26243-5

Insert

TNF receptor-associated factor 3 (TRAF3 Human)

Tags

1xHA

Expression

Mammalian

Mutation

Contains amino acids 334-568; S393G and K492N

Available Since

April 4, 2013

Availability

Academic Institutions and Nonprofits only
pPW3762 : CMVd3-HsIRE1(K121Y)-HaloTag

Plasmid

#185684

Purpose

Low-level transient expression of IF1 mutant HsIRE1 with a C-terminal HaloTag.

Depositor

Peter Walter

Article

Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

Insert

ERN1 (ERN1 Synthetic, Human)

Tags

HaloTag

Expression

Mammalian

Mutation

K121Y

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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p1.1-eGFP

Plasmid

#162738

Purpose

Fluorescent reporter for CHO cells expression studies

Depositor

Ivan Vorobiev

Article

Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:

Insert

enhanced green fluorescent protein

Expression

Mammalian

Mutation

consensus Kozak sequence (GCCGCCATGG) added befor…

Promoter

CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

Available Since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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pFSW Doc2alpha WT

Plasmid

#128812

Purpose

To make virus expressing Doc2alpha WT

Depositor

Edwin Chapman

Article

Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

Insert

Doc2alpha (Doc2a Rat)

Use

Lentiviral

Tags

IRES-EGFP

Promoter

hSyn (human synapsin I promoter)

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pFSW Doc2beta WT

Plasmid

#128814

Purpose

To make virus expressing Doc2beta WT

Depositor

Edwin Chapman

Article

Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

Insert

Doc2beta (Doc2b Rat)

Use

Lentiviral

Tags

IRES-EGFP

Promoter

hSyn (human synapsin I promoter)

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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Y587E EphA2 pcDNA3

Plasmid

#102730

Purpose

Expression of EphA2 in mammalian cells

Depositor

Jin Chen

Article

Fang et al J Biol Chem. 2008 Jun 6;283(23):16017-26

Insert

EphA2 (Epha2 Mouse)

Expression

Mammalian

Mutation

Y587E (TAT > GAG)

Promoter

CMV

Available Since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pRSET-pr-mEos4b (mEosFP4b-V69T)

Plasmid

#99226

Purpose

Bacterial expression of the primed conversion capable protein pr-mEos4b (mEos4b-V69T).

Depositor

Periklis Pantazis

Article

Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

Insert

pr-mEos4b

Expression

Bacterial

Mutation

V69T for primed conversion applications

Promoter

T7

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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mOrange2-Clathrin-15

Plasmid

#57947

Purpose

Localization: Clathrin Vesicles, Excitation: 549, Emission: 565

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Clathrin (CLTB Human)

Tags

mOrange2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2014

Availability

Academic Institutions and Nonprofits only
EGFP-Caveolin3DGV

Plasmid

#67886

Purpose

Fluorescently tagged Caveolin-3 truncation mutant for expression in mammalian cells

Depositor

Rob Parton

Article

Luetterforst et al J Cell Biol. 1999 Jun 28;145(7):1443-59.

Insert

Cav3 (Cav3 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Deletion of the first 53 amino acids of Caveolin3

Promoter

CMV

Available Since

Aug. 19, 2015

Availability

Academic Institutions and Nonprofits only
1352wUV2 F2bbs kan GCANNNAGGk

Plasmid

#18752

Depositor

Scot Wolfe

Article

Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701

Insert

3 tandem zinc fingers

Expression

Bacterial

Mutation

A Kan cassette (1090bp) is inserted between two b…

Available Since

July 11, 2008

Availability

Academic Institutions and Nonprofits only

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C1-F-tractin(delta1-6)-mCitrine

Plasmid

#155219

Purpose

F-actin bidning mutant of F-tractin

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

F-tractin (aa 9-52 of rat ITPKA) fused to mCitrine (Itpka Rat)

Tags

mCitrine

Expression

Mammalian

Mutation

Deletion of first 6 AA from F-tractin (9-14 from …

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-T3-st1CAS9-pA

Plasmid

#131542

Purpose

Expresses S. thermophilus 1-derived Cas9 nuclease in mammalian cells. Used to modify genome of mouse embroys

Depositor

Wataru Fujii

Article

Fujii et al Biochem Biophys Res Commun. 2016 Jun 16.

Insert

Codon optimized st1Cas9

Use

CRISPR

Tags

Flag, NLS, and polyA

Expression

Mammalian

Available Since

Nov. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mCherry-TSC2(AA)

Plasmid

#192442

Purpose

Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A mutations to block Akt phosphorylation.

Depositor

Jin Zhang

Article

Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

Insert

mCherry-TSC2(S939A,T1439A) (TSC2 Human)

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …

Promoter

CMV

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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