We narrowed to 9,400 results for: pho;
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Plasmid#108107PurposeLentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & TA mutation at LKB1 phosphorylation site) with neomycin resistance geneDepositorInsertSIK3 (SIK3 Human)
UseCRISPR and LentiviralExpressionMammalianMutationchange guanine 501 to cytosine (silent mutation),…PromoterEFS promoterAvailable SinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS405 PEX14-VAC8-RFP
Plasmid#166844PurposeExpresses Peroxisome localized Vac8-RFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 390 base pairs from the PEX14 ORFDepositorTagsPEX14 transmembrane domain and RFPExpressionYeastMutationPEX14 transmembrane domainPromoterVAC8 promoterAvailable SinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS405 SEC66-VAC8-RFP
Plasmid#166843PurposeExpresses ER localized Vac8-RFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 162 base pairs from the SEC66 ORF, which encode the Sec66 transmembrane domain.DepositorTagsRFP and SEC66 transmembrane domainExpressionYeastMutationSEC66 transmembrane domainPromoterVAC8 promoterAvailable SinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCerulean3-Integrin-Beta1-N-18
Plasmid#55423PurposeLocalization: Integrin/Focal Adhesions, Excitation: 433, Emission: 475DepositorAvailable SinceOct. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMH-HT_CtCHAD-H253A-R256A-R260A-R296A-R418A-Y419A
Plasmid#127790PurposeExpresses a CtCHAD mutant protein in E. coli cellsDepositorInsertCtCHAD (CT0884 Chlorobium tepidum)
Tags6xHis tag + TEV cleavage siteExpressionBacterialMutationResidues 208-522 (CHAD domain), mutations in His2…Available SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
YFP-Stim1-D76A
Plasmid#186622PurposeEncodes Stim1 mutant D76A fused to YFPDepositorInsertStromal Interaction Molecule 1 (STIM1 Human)
TagsYFPExpressionMammalianMutationD76APromoterCMVAvailable SinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEF1α-M2rtTA-T2A-Puro
Plasmid#169175PurposepiggyBac co-transfection transposon containing M2rtTA promoter linked with Puromycin resistance gene, through T2A with GSG linker, subcloned between ends of two ITRs. Pairs with #169174DepositorInsertM2rtTA-T2A-Puro
UsePiggybac transposonExpressionMammalianMutationSee Depositor Comments BelowPromoterEF1aAvailable SinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTOPO-MCL1S159A
Plasmid#21606DepositorInsertMCL-1 S159A (MCL1 Human)
ExpressionMammalianMutationSerine 159 mutated to Alanine, abolishing phosph…Available SinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCSIIbleo-PIF3-mEGFP
Plasmid#139483PurposeExpresses PIF3-mEGFP in mammalian cells.DepositorInsertPIF3 (PIF3 Mustard Weed)
UseLentiviralTagsmEGFPExpressionMammalianMutationPIF3 1-100 aaPromoterHuman EF1a promoterAvailable SinceApril 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro H-Ras 12V (37G)
Plasmid#12589DepositorAvailable SinceSept. 21, 2006AvailabilityAcademic Institutions and Nonprofits only -
Rtn4a-NTD1-135-mNeon
Plasmid#186609PurposeEncodes Reticulon 4 N-terminal domain aa 1 to 135 fused to mNeonGreenDepositorInsertHuman Reticulon 4 isoform A amino acids 1 to 135 (RTN4 Human)
TagsmNeonGreenExpressionMammalianPromoterCMVAvailable SinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmirGLO-Bmpr2-Δ17
Plasmid#49380PurposeInsert is a fragment of the Bmpr2 3'-UTR that contains a mutated miR-17 binding site. To be used for 3'UTR assay.DepositorInsertBmpr2 (BMPR2 Mouse, Human)
UseLuciferaseMutationchanged nucleotides AC to TG at bp 7347-7348PromoterHuman phosphoglycerate kinase (PGK) promoterAvailable SinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
p3F-Strep-hMIB2
Plasmid#33313DepositorAvailable SinceMarch 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
Sema4c-AP-His
Plasmid#72030PurposeExpresses the extracellular region of the Sema4C protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorAvailable SinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Fbxo7(ΔF-box)-IRES-GFP
Plasmid#171787Purposeretrovirus productionDepositorAvailable SinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMADM-alpha
Plasmid#36890DepositorInsertsGFP
tdTomato-3Myc
beta-globin intron
Neo
ATG (T)
GFP
Tags3 Myc tagsExpressionMammalianMutationdeleted nucleotides after nucleotide 274, deleted…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available SinceAug. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pT2EFCRET(#324)
Plasmid#184059Purposecyclofen-inducible CRE activation in zebrafish transient transgenicDepositorInsertCRE-ERT2
UseZebrafish transient transgenesisMutationERT2: G400V, M543A, L544A, deleted 1-281;PromoterXl-EF1aAvailable SinceJuly 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
Sema3a(L, del3)-AP-His
Plasmid#72010PurposeExpresses the Sema3A protein (truncated at cleavage site P3; ie, long and missing exon 3), C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorAvailable SinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLC-Flag-UBE2G1 sg2R-P2A-Hygro
Plasmid#124299PurposeLentiviral vector for expression of Flag tagged UBE2G1-P2A-Hygro casette from a CMV promoter. UBE2G1 cDNA contains silent mutations to disrupt a sgRNA binding site.DepositorInsertUBE2G1 (UBE2G1 Human)
UseLentiviralTagsFlagExpressionMammalianMutationseveral silent mutations to disrupt sgRNA targeti…PromoterCMVAvailable SinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
CMV-REX-GECO0.9
Plasmid#61247PurposeExpresses REX-GECO0.9 in mammalian cellsDepositorInsertREX-GECO0.9
ExpressionMammalianMutationSubstitutions relative to R-GECO1: P60R, V61W, R6…PromoterCMVAvailable SinceMarch 27, 2015AvailabilityAcademic Institutions and Nonprofits only