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pLNC Wnt-5b

Plasmid

#18023

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-5b (Wnt5b Mouse)

Use

Retroviral

Available Since

July 7, 2008

Availability

Academic Institutions and Nonprofits only
pHIV-IL13-CD8aStalk-PDGFR

Plasmid

#240242

Purpose

Lentiviral transfer plasmid encoding IL13 with CD8a stalk and PDGFR TM domain

Depositor

Michael Birnbaum

Article

Dobson et al Nat Methods. 2022 Apr;19(4):449-460. doi

Insert

IL-13 (IL13 Human)

Use

Lentiviral

Tags

EGFP and HA

Expression

Mammalian

Promoter

EF1a

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pHInt-AppA-KanR-loxP

Plasmid

#232910

Purpose

Plasmid for marker free integration of E. coli appA at the phac1 locus of Cupriavidus necator.

Depositor

Anita Emmerstorfer-Augustin

Article

Arhar et al ACS Synth Biol. 2025 Feb 24. doi: 10.102

Insert

AppA (appA )

Use

Synthetic Biology

Promoter

Pj5

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-drAgo2

Plasmid

#221712

Purpose

Expression of zebrafish Ago2 with N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2 (ago2 Zebrafish)

Use

Fish expression

Tags

3xFLAG

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pEXP5-NT/pLas eGFP

Plasmid

#193630

Purpose

Inducible expression of eGFP by the transcription factor complex LasR-3OC12 homoserine lactone (HSL).

Depositor

Dora Tang

Article

Gonzales et al ChemSystemsChem 2023

Insert

eGFP

Tags

6xHis

Expression

Bacterial

Promoter

T7 RNAP promoter

Available Since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA-ALFA-tag-Gal4-VP16

Plasmid

#199465

Purpose

Expression of the Gal4-ALFA-tag-VP16 synthetic transcription factor containing the ALFA-tag sequence in mammalian cells.

Depositor

Vladislav Verkhusha

Article

Chernov et al Int J Mol Sci. 2023 Mar 30;24(7):6526. d

Insert

Gal4-ALFA-tag-VP16

Expression

Mammalian

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pMUT2-VtrA-mCherry

Plasmid

#192862

Purpose

pMUT2 based vector for sensing human secondary bile acids in E. coli Nissle 1917

Depositor

Morten Sommer

Article

Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

Insert

VtrA-CadC fusion protein and cognate promoter inducible by secondary bile acids

Expression

Bacterial

Available Since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO-Tet-On shYAP1-3

Plasmid

#193666

Purpose

Tet inducible knockdown of YAP1

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1 puro shYAP1-1

Plasmid

#193692

Purpose

Constitutive lentiviral expression of YAP1 shRNA

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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EFS eGFP hPGK BSD

Plasmid

#188520

Purpose

Backbone for CRISPR interference (CRISPRi) isogenic PAM testing

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Type

Empty backbone

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pH6-spTorA-mCherry

Plasmid

#169040

Purpose

mCherry preceeded by the signal peptide of TorA (spTorA) with an N-terminal 6xHis tag in pET28a.

Depositor

Siegfried Musser

Article

Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi

Insert

H6-spTorA-mCherry

Tags

6xHis and spTorA (signal peptide of E. coli TorA)

Expression

Bacterial

Promoter

T7

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pSPCas9(BB)-2A-GFP_ABCA3GFP_targeting

Plasmid

#188541

Purpose

Plasmid encoding pCas9 and gRNA targeting the endogenous human ABCA3 locus stop codon

Depositor

Darrell Kotton

Article

Sun et al Am J Respir Cell Mol Biol. 2021 Oct;65(4

Insert

gRNA

Use

CRISPR

Tags

GFP

Promoter

U6

Available Since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA TfR1-miniE

Plasmid

#181987

Purpose

MiniE genetically encoded tag on Transferrin Receptor 1 (TfR1) to be used with MiniR labeled with a reporter

Depositor

Kimberly Beatty

Article

Doh et al Biochemistry. 2020 Aug 25;59(33):3051-30

Insert

TfR1-MiniE

Expression

Mammalian

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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L2_UBE2:mT-N7-CsA

Plasmid

#136133

Purpose

Plasmid to transform Mp with constitutive promoter driving mTurq to the nuclei. HygR

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-35S:HygR p5-MpUBE2:mTurq-N7

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-CTSS

Plasmid

#172415

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

Cathepsin S (CTSS Human)

Tags

MAC-tag

Expression

Mammalian

Promoter

CMV promoter

Available Since

Nov. 4, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pMAL728

Plasmid

#169152

Purpose

Encodes OptoQ-AMP6

Depositor

Jose Avalos

Article

Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

Insert

VP16-EL222

Tags

SV40 NLS

Expression

Yeast

Mutation

A79Q

Available Since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pSEVA221TrxA-AID-T7RNAP

Plasmid

#167976

Purpose

Expresses fusion protein TrxA-AID-T7RNAP in E. coli

Depositor

Luis Ángel Fernández

Article

Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

Insert

TrxA-AID-T7RNAP

Tags

T7RNAP and TrxA

Expression

Bacterial

Promoter

Ptet

Available Since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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sgCTNNB1 Nicking

Plasmid

#169845

Purpose

Express a nicking sgRNA used for installation of the oncogenic S45F or S45del in Ctnnb1 in mouse liver.

Depositor

Wen Xue

Article

Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

Insert

sgCTNNB1 Nicking (Ctnnb1 Mouse)

Expression

Mammalian

Available Since

June 4, 2021

Availability

Academic Institutions and Nonprofits only

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pegCTNNB1 S45del

Plasmid

#169844

Purpose

pegRNA used for installation (via TCC deletion) of the oncogenic S45del in Ctnnb1 in mouse liver.

Depositor

Wen Xue

Article

Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

Insert

pegCTNNB1 S45DEL (Ctnnb1 Mouse)

Expression

Mammalian

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pegCTNNB1 S45F

Plasmid

#169843

Purpose

pegRNA used for installation (via C•G-to-T•A) of the oncogenic S45F in Ctnnb1 in mouse liver.

Depositor

Wen Xue

Article

Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

Insert

pegCTNNB1 S45F (Ctnnb1 Mouse)

Expression

Mammalian

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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