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PROM5_MpEF1a

Plasmid

#136113

Purpose

Strong promoter from MpEF1a, that drives expression everywhere in Mp gemmae but stronger in the notches

Depositor

Jim Haseloff

Publication

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

PROM5_MpEF1a Mapoly0024s0116

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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3TERM_Nos-35S

Plasmid

#136116

Purpose

3TERM after CDS/ CDS12-CTAG

Depositor

Jim Haseloff

Publication

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

3TERM_Nos-35S (A. tumefaciens, CaMV)

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pSIN-Ldha

Plasmid

#175939

Purpose

knockout mouse Ldha

Depositor

Dan Littman

Publication

Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

Insert

Ldha gRNA (Ldha Mouse)

Use

Retroviral

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pT2MP.EF-QUE37C

Plasmid

#129332

Purpose

ATP measurement, Tol2 transposon

Depositor

Yasushi Okada

Publication

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

QUEEN-37C

Expression

Mammalian

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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SNX11-PX (7-167)

Plasmid

#119092

Purpose

Bacterial expression of human phox homology (PX) domain, SNX11-PX (7-167)

Depositor

Brett Collins

Publication

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SNX11-PX (7-167) (SNX11 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pNOC-stacked-GeNL

Plasmid

#101002

Purpose

Construct for fusion expression of GeNL reporter and neomycin/G418 resistance gene by a bidirectional promoter

Depositor

Eva Farre

Publication

Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

Type

Empty backbone

Use

Algae, nannochloropsis

Promoter

Ribi

Available since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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pRG/III-hs-MBP

Plasmid

#121676

Purpose

Bacterial expression of MBP fusion proteins

Depositor

Reinhard Grisshammer, Richard Henderson

Publication

Tucker et al Biochem J. 1996 Aug 1;317 ( Pt 3):891-9.

Insert

maltose binding protein

Expression

Bacterial

Promoter

lac promoter

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pNP1 Aqua toehold switch

Plasmid

#107356

Purpose

Expresses Aquamarine (an eCFP derivative) in Ecoli off a T7 promoter when pNP1 Aqua trigger is co-expressed

Depositor

James Collins

Publication

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

toehold switch 8 + Aquamarine

Expression

Bacterial

Promoter

T7

Available since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pOGG037

Plasmid

#113995

Purpose

SC module. Super-folder GFP green fluorescence reporter

Depositor

Philip Poole

Publication

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

Super-folder GFP

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pColE1_sgRNA_pos9_AmpR

Plasmid

#119152

Purpose

Encodes sgRNA targeting position 9 in pColE1_70a_deGFP_KanR

Depositor

Vincent Noireaux

Publication

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sgRNA targeting position 9 in pColE1_70a_deGFP_KanR

Use

Crispr

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-ldhA

Plasmid

#102288

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting ldhA.

Depositor

Michael Lynch

Publication

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

ldhA gRNA

Use

CRISPR

Expression

Bacterial

Promoter

pTet

Available since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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pShuttle-FEN1mED

Plasmid

#35033

Depositor

Sheila Stewart

Publication

Saharia et al J Biol Chem. 2010 Aug 27;285(35):27057-6

Insert

FEN1mWT

Use

Adenoviral

Tags

Flag

Expression

Mammalian

Mutation

PT MUTANT E160D

Promoter

CMV

Available since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only
pE1B-C-F/Sox10-MCS9

Plasmid

#20248

Depositor

William Pavan

Publication

Antonellis et al PLoS Genet. 2008 . 4(9):e1000174.

Insert

Sox10-MCS9

Expression

Mammalian

Available since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

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pCAG-EGFP/RFP-miR-E1-1int

Plasmid

#19819

Depositor

Zuoshang Xu

Publication

Qiu et al BMC Biotechnol. 2008 Oct 7;8:77. doi: 10

Insert

miRNA

Use

Cre/Lox and RNAi

Expression

Mammalian

Available since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
pCAG-EGFP/RFP-miR-E2-4int

Plasmid

#19820

Depositor

Zuoshang Xu

Publication

Qiu et al BMC Biotechnol. 2008 Oct 7;8:77. doi: 10

Insert

miRNA

Use

Cre/Lox and RNAi

Expression

Mammalian

Available since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
pTT5-hSDC1-N20

Plasmid

#52355

Purpose

expresses human syndecan 1 'del 1- 20' truncation mutant in HEK293-EBNA1 (293E) suspension culture.

Depositor

Gordon Laurie

Publication

Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SDC1 (SDC1 Human)

Expression

Mammalian

Mutation

Deletion of SDC1 aa2-21 (according to numbering i…

Promoter

CMV

Available since

Dec. 8, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pQCXIP-LAMP1-EGFP-APEX2-V5

Plasmid

#236402

Purpose

Expresses a fluorescent lysosome-localized APEX2

Depositor

Jared Rutter

Publication

Cunningham et al J Cell Sci. 2025 Mar 13:jcs.263693. doi:

Insert

LAMP1-EGFP-APEX2-V5

Use

Retroviral

Tags

APEX2, EGFP, and V5

Expression

Mammalian

Available since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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BcLOV-mCh-EGFR

Plasmid

#208274

Purpose

Expresses BcLOV-EGFR for optogenetic activation of EGFR signaling. Includes an mCherry tag.

Depositor

Lukasz Bugaj

Publication

Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

Insert

BcLOV-mCherry-EGFR

Use

Lentiviral

Tags

mCherry

Promoter

pCMV

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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Diffusive 27kDa (EGFP)

Plasmid

#201362

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Publication

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

EGFP

Expression

Mammalian

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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H_NES 41kDa

Plasmid

#201346

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Publication

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

HIV_NES-EGFP-2PrA

Expression

Mammalian

Available since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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