We narrowed to 13,054 results for: Mpl

1. pFastBac-hDcr-K70A.

   Plasmid

   #89145

   Purpose

   Expression of helicase mutant of human Dicer in Sf9 cells

   Depositor

   Jennifer Doudna

   Article

   MacRae et al Proc Natl Acad Sci U S A. 2008 Jan 15;10

   Insert

   hDicer (DICER1 Human)
   

   Use

   Tags

   His6-tev

   Expression

   Insect

   Mutation

   K70A

   Promoter

   Available since

   April 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. P21-sp64 Poly A

   Plasmid

   #59433

   Purpose

   synthetic poly A transcripts of VZV

   Depositor

   Randall Cohrs

   Article

   Cohrs et al J Virol. 2000 Dec;74(24):11464-71.

   Insert

   VZV ORF 21
   

   Use

   Makes synthetic transcripts that contains poly a

   Tags

   none

   Expression

   Mutation

   none

   Promoter

   SP6

   Available since

   Sept. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

3. HLH Bmal Pas 1-465-VenC

   Plasmid

   #47364

   Purpose

   Bmal fragment cloned with Venus tag and used for BiFC

   Depositor

   Joseph Takahashi

   Article

   Huang et al Science. 2012 Jul 13;337(6091):189-94. d

   Insert

   BMAL (Bmal1 Mouse)
   

   Use

   Tags

   Venus aa 156-239

   Expression

   Mammalian

   Mutation

   aa 1-465

   Promoter

   CMV

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

4. FHpHG_mTent5c_LxxAA (L3m)

   Plasmid

   #221441

   Purpose

   Transfection into the cells for the reporter or qPCR assay.

   Depositor

   Marcos Morgan

   Article

   Li et al FEBS J. 2025 May 27. doi: 10.1111/febs.7

   Insert

   Tent5c_LXXAA (L3m) (Fam46c Mouse)
   

   Use

   Tags

   FLAG-2xHA-(HRV 3C site)-6xHis-EGFP

   Expression

   Mammalian

   Mutation

   L332A, LL335-336AA (CTC atc tct CTC CTG to CTC at…

   Promoter

   Available since

   June 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. TTR F87E

   Plasmid

   #238075

   Purpose

   Bacterial expression of human TTR mutants

   Depositor

   Peter Wright

   Article

   Leach et al J Mol Biol. 2024 Aug 15;436(16):168673.

   Insert

   TTR (TTR Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   F87E

   Promoter

   T7

   Available since

   June 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pETM11-6xHis-TEV-hORF1p

   Plasmid

   #202589

   Purpose

   Allows for IPTG-inducible expression of the human L1RP ORF1 protein in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scar

   Depositor

   Kathleen Burns, Liam Holt

   Article

   Taylor et al Cell. 2013 Nov 21;155(5):1034-48. doi: 1

   Insert

   ORF1p
   

   Use

   Tags

   6x His and TEV protease cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA4c/His-Xpress-HA-hBIG2(WT)

   Plasmid

   #79432

   Purpose

   Expresses N-terminally His6, Xpress and HA-tagged BIG2(WT) in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Shin et al Mol Biol Cell. 2004 Dec;15(12):5283-94.

   Insert

   BIG2 (ARFGEF2 Human)
   

   Use

   Tags

   HA, His6, and Xpress

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA4c/His-Xpress-HA-hBIG1(WT)

   Plasmid

   #79431

   Purpose

   Expresses N-terminally His6, Xpress and HA-tagged BIG1(WT) in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Shin et al Mol Biol Cell. 2004 Dec;15(12):5283-94.

   Insert

   BIG1 (ARFGEF1 Human)
   

   Use

   Tags

   HA, His6, and Xpress

   Expression

   Mammalian

   Mutation

   C1758R

   Promoter

   Available since

   June 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC18 3xFLAG COP1

   Plasmid

   #237334

   Purpose

   expression in bacterial cells for protein purification

   Depositor

   Michele Pagano

   Article

   Rizzini et al Curr Biol. 2019 Jun 17;29(12):1954-1962.

   Insert

   COP1 (COP1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSMPPv2-Tau-RING (M72E/I18R)

    Plasmid

    #233690

    Purpose

    Expression of Tau-RING I18R-M72E mutant in mammalian cells

    Depositor

    Leo James

    Article

    Miller et al Cell. 2024 Oct 17;187(21):5967-5980.e17.

    Insert

    Tau protein fused to TRIM21 RING domain carrying M72E/I18R mutations
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOPTG-Tau-RING (M72E/I18R)

    Plasmid

    #233692

    Purpose

    Expression of Tau-RING I18R-M72E mutant in bacterial cells

    Depositor

    Leo James

    Article

    Miller et al Cell. 2024 Oct 17;187(21):5967-5980.e17.

    Insert

    Tau protein fused to TRIM21 RING domain
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRDA355_sgCiPOLR2D

    Plasmid

    #229022

    Purpose

    Expression of a CRISPRi doxycycline inducible guide targeting POLR2D

    Depositor

    Francisca Vazquez

    Article

    Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

    Insert

    POLR2D gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. OPEN-HLA-A*11:01-BirA

    Plasmid

    #235135

    Purpose

    E. coli expression of BirA tagged HLA-A*11:01 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin.

    Depositor

    Nikolaos Sgourakis

    Article

    Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

    Insert

    OPEN-HLA-A*11:01 (HLA-A Human)
    

    Use

    Tags

    BirA

    Expression

    Bacterial

    Mutation

    G120C

    Promoter

    Available since

    April 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRDA355_sgCiPELO #2

    Plasmid

    #229020

    Purpose

    Expression of a CRISPRi doxycycline inducible guide targeting PELO

    Depositor

    Francisca Vazquez

    Article

    Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

    Insert

    PELO gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRDA052H_sgCh2

    Plasmid

    #229023

    Purpose

    Expression of a EnAsCas12a control guide that cuts an intergenic region on chromosome 2

    Depositor

    Francisca Vazquez

    Article

    Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

    Insert

    sgChr2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRDA_174_neoR

    Plasmid

    #228935

    Purpose

    Lentiviral expression of EnAsCas12a

    Depositor

    Francisca Vazquez

    Article

    Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRDA052H_sgFOCAD #2

    Plasmid

    #229024

    Purpose

    Expression of a EnAsCas12a guide targeting FOCAD

    Depositor

    Francisca Vazquez

    Article

    Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

    Insert

    FOCAD gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pXPR_051d_sgCiChr2-2

    Plasmid

    #228940

    Purpose

    Expression of a CRISPRi control sgRNA that cuts an intergenic region on chromosome 2

    Depositor

    Francisca Vazquez

    Article

    Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

    Insert

    sgChr2-2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTMiniTrex-mCherry-Shark3

    Plasmid

    #233145

    Purpose

    Serves as a template plasmid for amplifying Shark3 (Theo-OFF) hammerhead aptazyme-3xTy-tagging cassette

    Depositor

    Ronald Etheridge

    Article

    Wiedeman et al Proc Natl Acad Sci U S A. 2025 Feb 25;12

    Inserts

    mCherry
    Shark3
    

    Use

    Prokaryotic expression

    Tags

    3x Ty tag

    Expression

    Mutation

    Codon optimized for Trypanosoma cruzi and changed…

    Promoter

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-myc:FRB:gs10:Cul1(dC501)

    Plasmid

    #228533

    Purpose

    A fragment of humanCul1 protein in fusion with FRB domain through a flexible glycine-serine linker.

    Depositor

    Roman Jerala

    Article

    Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80

    Insert

    myc:FRB:gs10:Cul1(dC501) (CUL1 Synthetic, Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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