Addgene

Showing: 7101 - 7120 of 23124 results

Results per page:

Sort results by:

pHR-BRD4ΔN-mCherry-sspB

Plasmid

#121968

Purpose

Expresses fusion of disordered protein BRD4(462-1362), fluorescent protein mCherry, and sspB which upon light activation binds to iLID.

Depositor

Clifford Brangwynne

Article

Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

Insert

BRD4 (BRD4 Human)

Use

Lentiviral

Tags

mCherry-sspB

Expression

Mammalian

Mutation

Deleted amino acids 1-461

Promoter

SFFV

Available since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
TetO-FUW-MEF2CA

Plasmid

#61538

Purpose

Expresses MEF2CA in mammalian cells

Depositor

Rudolf Jaenisch

Article

Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

Insert

MEF2CA (MEF2C Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only
pOPINE GFP nanobody:Halo:His6

Plasmid

#111090

Purpose

Bacterial expression of a fusion protein consisting of a GFP nanobody (Dr. Brett Collins, Addgene plasmid # 49172), the Halo tag and a His6 tag.

Depositor

Lennart Wirthmueller

Article

Chen et al Front Plant Sci. 2018 Nov 1;9:1581. doi:

Insert

GFP Nanobody

Use

Tags

Halo and His6

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

T7 promoter

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-LC3

Plasmid

#21073

Purpose

Mammalian expression of rat LC3 fused to EGFP

Depositor

Tamotsu Yoshimori

Article

Kabeya et al EMBO J. 2000 Nov 1. 19(21):5720-8.

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

First ATG is not included.

Promoter

CMV

Available since

May 11, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti CMV TRE3G Puro FLAG-DD-ER-mCherry-TRF1-FokI D450A

Plasmid

#207953

Purpose

nuclease inactive control for TRF1FokI WT

Depositor

Roger Greenberg

Article

Dilley et al Nature. 2016 Nov 3;539(7627):54-58. doi:

Insert

TRF1 (TERF1 Human)

Use

Lentiviral

Tags

Flag, DD, ER, mCherry and FokI (D450A)

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
phROSA26-Tet-HA-JSAP1_WT

Plasmid

#208772

Purpose

Tetracycline inducible expression vector for HA-JSAP1_WT, used as a donor plasmid for HA-JSAP1_WT knock-in at the human ROSA26 locus

Depositor

Katsuji Yoshioka

Article

Suzuki et al Genes Cells. 2023 Nov 14. doi: 10.1111/g

Insert

JSAP1 (MAPK8IP3 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hsyn-GRAB_DA2h

Plasmid

#140554

Purpose

Expresses the 2nd generation high-affinity DA sensor GRAB_DA2h in neurons

Depositor

Yulong Li

Article

Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

Has Service

AAV9

Insert

The 2nd generation DA sensor GRAB_DA2h (high affinity)

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

July 29, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-PGK-PYL1-sfGFP-Coilin

Plasmid

#122177

Purpose

expression of PYL1-SfGFP-Coilin, lentivirus vector

Depositor

Stanley Qi

Article

Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

Insert

Coilin (COIL Human)

Use

Lentiviral

Tags

PYL1-SFGFP

Expression

Mammalian

Mutation

Promoter

PGK

Available since

April 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Luc-PS9

Plasmid

#177942

Purpose

Transient mammalian expression of Luc2 (firefly luciferase) along with SARS-CoV-2 sequence 20080-21171 (PS9) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

Luc2 (ORF1ab )

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pKLV-EF1a-mCherryLuciferase-W

Plasmid

#200101

Purpose

Lentiviral vector expressing mCherry fused with Luciferase at the C-terminus

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

Luciferase

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAY15 (OsTIR1(F74G) mAID-EGFP-NLS)

Plasmid

#140534

Purpose

OsTIR1(F74G)-P2A-mAID-EGFP-NLS TOL2

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

OsTIR1(F74G) mAID-EGFP-NLS TOL2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MET7A-CD4d3+4-bio

Plasmid

#73127

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding MET7A with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

MET7A (METTL7A Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
p3xFLAG-CMV14/p14ARF

Plasmid

#78760

Purpose

To overexpress p14ARF in Mammalian Cells

Depositor

Jaewhan Song

Article

Ko et al J Natl Cancer Inst. 2012 Nov 7;104(21):1

Insert

ARF (CDKN2A Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 9, 2016

Availability

Academic Institutions and Nonprofits only
p6XHis_NLS-SaCas9

Plasmid

#101086

Purpose

Express SaCas9 in bacteria with a 6xHis tag for purification

Depositor

Rick Tarleton

Article

Soares Medeiros et al MBio. 2017 Nov 7;8(6). pii: e01788-17. d

Insert

CRISPR-associated protein Cas9/Csn1

Use

CRISPR

Tags

TrxA-6xHis-STag

Expression

Bacterial

Mutation

Promoter

T7lac

Available since

Dec. 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDisplay-GRAB_eCB2.0-IRES-mCherry-CAAX

Plasmid

#164611

Purpose

Express the endocannabinoid sensor GRAB_eCB2.0 in mammalian cells

Depositor

Yulong Li

Article

Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

Insert

Endocannabinoid sensor GRAB_eCB2.0

Use

Tags

Expression

Mammalian

Mutation

S383T

Promoter

CMV

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hsyn-GRAB_rDA1m

Plasmid

#140556

Purpose

Expresses the red fluorescent medium-affinity DA sensor GRAB_rDA1m in neurons

Depositor

Yulong Li

Article

Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

Has Service

AAV9

Insert

Red fluorescent DA sensor GRAB_rDA1m (medium affinity)

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

July 29, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
GABA(A) receptor subunit g2SE

Plasmid

#49170

Purpose

pHluorin-tagged GABA A receptor subunit (gamma 2) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surface

Depositor

Tija Jacob, Stephen Moss

Article

Jacob et al J Neurosci. 2005 Nov 9;25(45):10469-78.

Insert

GABA(A) receptor subunit gamma 2 (Gabrg2 Mouse)

Use

Tags

myc, pHGFP (pHluorin), and thrombin cleavage site…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pIGAhRC

Plasmid

#112510

Purpose

human Ah receptor and Arnt cDNAs expressed under control of Gal1,10 bidirectional promoter

Depositor

Charles Miller

Article

Miller CA et al Toxicol Appl Pharmacol. 1999 Nov 1;160(3

Insert

HIF1B, AhR (ARNT, AHR Budding Yeast, Human)

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Gal1,10

Available since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Braf

Plasmid

#40775

Depositor

Dustin Maly

Article

Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

Insert

Braf (BRAF Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Starting Met removed

Promoter

CMV

Available since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pDisplay-GRAB_eCBmut-IRES-mCherry-CAAX

Plasmid

#164612

Purpose

Express the endocannabinoid non-responsive sensor GRAB_eCB2.0 in mammalian cells

Depositor

Yulong Li

Article

Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

Insert

Endocannabinoid non-responsive sensor GRAB_eCBmut

Use

Tags

Expression

Mammalian

Mutation

S383T, F177A

Promoter

CMV

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge