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pcDNA3_N-DYK_MTHFD1-NLS

Plasmid

#133023

Purpose

NLS sequence was fused to MTHFD1-wild type through site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, New England Biolabs)

Depositor

Stefan Kubicek

Article

Sdelci et al Nat Genet. 2019 Jun;51(6):990-998. doi:

Insert

MTHFD1 (MTHFD1 Human)

Tags

NLS

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223_ERBB2_WT

Plasmid

#82300

Purpose

Gateway Donor vector containing ERBB2, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ERBB2 (ERBB2 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_AR_WT

Plasmid

#82094

Purpose

Gateway Donor vector containing AR , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

AR (AR Human)

Use

Gateway entry vector

Mutation

p.81_82insQQ; deletion of AA397-482

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CBX1_WT

Plasmid

#82102

Purpose

Gateway Donor vector containing CBX1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CBX1 (CBX1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_MAPK3_WT

Plasmid

#82313

Purpose

Gateway Donor vector containing MAPK3 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MAPK3 (MAPK3 Human)

Use

Gateway entry vector

Mutation

L180X; p.135_136delSQ-insRPAAE; p.138_379del

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_HRAS_p.Q61K

Plasmid

#81319

Purpose

Gateway Donor vector containing HRAS , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

HRAS (HRAS Human)

Use

Gateway entry vector

Mutation

Q61K

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCW-eGFP-SHLD2-m1

Plasmid

#114120

Purpose

Lentiviral vector for inducible expression of N-terminally tagged eGFP-SHLD2, m1 mutant

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Insert

SHLD2 (m1 mutant) (SHLD2 Human)

Use

Lentiviral; Doxycycline inducible

Tags

eGFP

Expression

Mammalian

Mutation

W489A, F494A, W495A mutations introduced

Promoter

TRE promoter, Tet ON

Available Since

Aug. 28, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223_AKT1_p.E17K

Plasmid

#81459

Purpose

Gateway Donor vector containing AKT1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

AKT1 (AKT1 Human)

Use

Gateway entry vector

Mutation

E17K

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_KRAS_p.A59G

Plasmid

#81662

Purpose

Gateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

KRAS (KRAS Human)

Use

Gateway entry vector

Mutation

A59G

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pJT230 lenti pEF-rTetR(SE-G72P)-3XFLAG-NCOA3-TAD-T2A-mCherry-BSD-WPRE

Plasmid

#188763

Purpose

Lentiviral backbone containing NCOA3 as a fusion with rTetR(SE-G72P)-3XFLAG

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

NCOA3 (NCOA3 Human)

Use

Lentiviral

Tags

rTetR(SE-G72P)-3xFLAG

Expression

Mammalian

Promoter

pEF1a

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_ERBB2_WT

Plasmid

#82113

Purpose

Gateway Donor vector containing ERBB2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ERBB2 (ERBB2 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pGLUE-SHLD2

Plasmid

#114118

Purpose

Expresses N-terminally tagged Streptag-HA-CBP-SHLD2 in mammalian cells

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Insert

SHLD2 (SHLD2 Human)

Tags

HA tag, Streptavidin-binding tag (Streptag), and …

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 28, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pRK5-mEGFP-RAX-MUT

Plasmid

#194573

Purpose

Mammalian Expression of mEGFP-RAX Mutant (S222Rfs*63) Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

RAX (RAX Human)

Tags

mEGFP

Expression

Mammalian

Mutation

S222Rfs*63

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-MYOD1-MUT

Plasmid

#194571

Purpose

Mammalian Expression of mEGFP-MYOD1 Mutant (R188Pfs*90) Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

MYOD1 (MYOD1 Human)

Tags

mEGFP

Expression

Mammalian

Mutation

R188Pfs*90

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3_N-DYK_MTHFD1-NES

Plasmid

#133022

Purpose

NES sequence was fused to MTHFD1-wild type through site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, New England Biolabs)

Depositor

Stefan Kubicek

Article

Sdelci et al Nat Genet. 2019 Jun;51(6):990-998. doi:

Insert

MTHFD1 (MTHFD1 Human)

Tags

NES

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pINDUCER20 MEF2C S222A

Plasmid

#89718

Purpose

Lentiviral gene expression vector for doxycycline inducible MEF2C-S222A expression

Depositor

Alex Kentsis

Article

Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

Insert

MEF2C (MEF2C Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Changed serine 222 to alanine

Available Since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_KRAS_p.G12A

Plasmid

#81673

Purpose

Gateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

KRAS (KRAS Human)

Use

Gateway entry vector

Mutation

G12A

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_HRAS_p.G12C

Plasmid

#81266

Purpose

Gateway Donor vector containing HRAS , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

HRAS (HRAS Human)

Use

Gateway entry vector

Mutation

G12C

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_KPNA3_p.V154G

Plasmid

#81457

Purpose

Gateway Donor vector containing KPNA3 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

KPNA3 (KPNA3 Human)

Use

Gateway entry vector

Mutation

A152D; V153I; V154G; P332Q; N411D

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_MAP2K4_WT

Plasmid

#82142

Purpose

Gateway Donor vector containing MAP2K4 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MAP2K4 (MAP2K4 Human)

Use

Gateway entry vector

Mutation

P27R; p.8delG

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-racRNA-B

Plasmid

#200825

Purpose

AAV transfer plasmid encoding a circularized RNA barcode B under U6 promoter and a subcellular localization protein binder under hSyn promoter

Depositor

Xiao Wang

Article

Shi et al Nature. 2023 Oct;622(7983):552-561. doi:

Inserts

U6-racRNA
V5-PP7cp-M9-NES-T2A-Flag-PP7cp-Far

Use

AAV

Tags

C-Far, Flag, and V5

Expression

Mammalian

Promoter

hSyn

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-racRNA-C

Plasmid

#200826

Purpose

AAV transfer plasmid encoding a circularized RNA barcode C under U6 promoter and a subcellular localization protein binder under hSyn promoter

Depositor

Xiao Wang

Article

Shi et al Nature. 2023 Oct;622(7983):552-561. doi:

Inserts

U6-racRNA
V5-PP7cp-M9-NES-T2A-Flag-PP7cp-Far

Use

AAV

Tags

C-Far, Flag, and V5

Expression

Mammalian

Promoter

hSyn

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-racRNA-D

Plasmid

#200827

Purpose

AAV transfer plasmid encoding a circularized RNA barcode D under U6 promoter and a subcellular localization protein binder under hSyn promoter

Depositor

Xiao Wang

Article

Shi et al Nature. 2023 Oct;622(7983):552-561. doi:

Inserts

U6-racRNA
V5-PP7cp-M9-NES-T2A-Flag-PP7cp-Far

Use

AAV

Tags

C-Far, Flag, and V5

Expression

Mammalian

Promoter

hSyn

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_HRAS_p.Q61R

Plasmid

#81311

Purpose

Gateway Donor vector containing HRAS , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

HRAS (HRAS Human)

Use

Gateway entry vector

Mutation

Q61R

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_AKT3_WT

Plasmid

#82093

Purpose

Gateway Donor vector containing AKT3 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

AKT3 (AKT3 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pWZL Neo Myr Flag AMHR2

Plasmid

#20424

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

AMHR2 (AMHR2 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

C473G mutation when compared to Genbank reference…

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pDONR223_IDH2_p.R172M

Plasmid

#81618

Purpose

Gateway Donor vector containing IDH2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

IDH2 (IDH2 Human)

Use

Gateway entry vector

Mutation

R172M

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_AKT1_p.Q79K

Plasmid

#81479

Purpose

Gateway Donor vector containing AKT1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

AKT1 (AKT1 Human)

Use

Gateway entry vector

Mutation

Q79K

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_KRAS_p.D33E

Plasmid

#81659

Purpose

Gateway Donor vector containing KRAS,part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

KRAS (KRAS Human)

Use

Gateway entry vector

Mutation

D33E

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_MAPK6_p.D387N

Plasmid

#81712

Purpose

Gateway Donor vector containing MAPK6, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MAPK6 (MAPK6 Human)

Use

Gateway entry vector

Mutation

L290V; D387N; del amino acids 1-8

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge