We narrowed to 18,871 results for: IRE

1. pMSP1D1

   Plasmid

   #20061

   Purpose

   Bacterial expression of membrane scaffold protein (MSP1D1) for Nanodisc formation

   Depositor

   Stephen Sligar

   Article

   Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

   Insert

   MSP1D1
   

   Use

   Tags

   7-His tag

   Expression

   Bacterial

   Mutation

   deletion mutant (1-11) of MSP1; His-tag on N-term…

   Promoter

   Available since

   March 16, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pXR003: CasRx gRNA cloning backbone

   Plasmid

   #109053

   Purpose

   hU6-driven expression of guide RNAs compatible with CasRx. 5' processed DR followed by BbsI sites for guide cloning.

   Depositor

   Patrick Hsu

   Article

   Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMSCVhyg-3xT7-CA-ChREBP

   Plasmid

   #171029

   Purpose

   Expresses 3xT7-tagged constitutive active form of ChREBP in mammalian cells

   Depositor

   Kai Ge

   Article

   Jang et al Genes Dev. 2021 Apr 22. pii: gad.347583.

   Insert

   CA-ChREBP (Mlxipl Mouse)
   

   Use

   Retroviral

   Tags

   3xT7

   Expression

   Mammalian

   Mutation

   amino acids 240 - 864

   Promoter

   5' LTR

   Available since

   Aug. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pT7-EGFP-C1-HsXRN1_K

   Plasmid

   #146784

   Purpose

   Mammalian Expression of HsXRN1

   Depositor

   Elisa Izaurralde

   Article

   Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

   Insert

   HsXRN1 (XRN1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. p11-LacY-wtx1

   Plasmid

   #69056

   Purpose

   The reporter plasmid p11-LacY-wtx1 encodes the toxin CcdB gene under the inducible BAD promoter and a single copy of the endonuclease cleavage site which is readily extendable to multiple copies.

   Depositor

   Huimin Zhao

   Article

   Chen et al Nucleic Acids Res. 2005 Oct 6;33(18):e15

   Inserts

   ccdB (ccdB )
   lacY (lacY )
   

   Use

   Tags

   A177C

   Expression

   Mutation

   Promoter

   pBAD and pLac

   Available since

   Sept. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-Flag mTOR wt

   Plasmid

   #26603

   Purpose

   Mammalian expression of human flag-tagged mTor

   Depositor

   Jie Chen

   Article

   Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72

   Insert

   mTOR (MTOR Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 28, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti_EF1a_Flag_EGFP

   Plasmid

   #183117

   Purpose

   Expresses flag-tagged EGFP in mammalian cells

   Depositor

   William Kaelin

   Article

   Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

   Insert

   EGFP
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJH2198

   Plasmid

   #179773

   Purpose

   Prgef-1 NLF-1::GFP unc-54 3' UTR C.elegans pan-neural expression of NLF-1::GFP

   Depositor

   Mei Zhen

   Article

   Xie et al Neuron. 2013 Mar 20;77(6):1069-82. doi:

   Insert

   NLF-1::GFP (nlf-1 Nematode)
   

   Use

   Tags

   GFP

   Expression

   Worm

   Mutation

   Promoter

   Prgef-1

   Available since

   March 14, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pLenti_EF1a_EV

   Plasmid

   #183116

   Purpose

   Empty vector control for pLenti_EF1a constructs

   Depositor

   William Kaelin

   Article

   Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

   Insert

   Small multiple cloning site
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pICH47742::p35S-ePEmax3::tEURb7

    Plasmid

    #227692

    Purpose

    Golden Gate level 1, position 2, ePEmax3 expression cassette.

    Depositor

    Jae-Yean Kim

    Article

    Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

    Insert

    ePEmax3 being driven by long CaMV 35S (p35S) and terminated by a dual terminator (EURb7).
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available since

    Nov. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. Luciferase-pcDNA3

    Plasmid

    #18964

    Depositor

    William Kaelin

    Article

    Safran et al Proc Natl Acad Sci U S A. 2006 Jan 3. 10

    Insert

    Firefly Luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLVX-3XMyc-mTrim25

    Plasmid

    #51394

    Depositor

    Narry Kim

    Article

    Kwon et al Nat Struct Mol Biol. 2013 Sep;20(9):1122

    Insert

    Trim25 (Trim25 Mouse)
    

    Use

    Lentiviral

    Tags

    3XMyc

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pICH47742:: pRPS5a-PE2max-NC::tHSP

    Plasmid

    #227694

    Purpose

    Golden Gate level 1, position 2, PE2max-NC expression cassette for Arabidopsis.

    Depositor

    Jae-Yean Kim

    Article

    Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

    Insert

    PE2max-NC being driven by pRPS5a and terminated by tHSP
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    pRPS5a

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. LeGO-dKatushka2

    Plasmid

    #85214

    Purpose

    Lentiviral vector expressing dKatushka2 fluorescent protein driven by SFFV promoter

    Depositor

    Boris Fehse

    Article

    Mohme et al Mol Ther. 2017 Jan 18. pii: S1525-0016(1

    Insert

    dKatushka2
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    BsrGI site removed at 5' end, BsrGI site ins…

    Promoter

    SFFV

    Available since

    Jan. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. GFP-p53

    Plasmid

    #12091

    Depositor

    Tyler Jacks

    Article

    Boyd et al Nat Cell Biol. 2000 Sep . 2(9):563-8.

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    72R variant of p53

    Promoter

    Available since

    July 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET-11b_PcrA_M6_H93A

    Plasmid

    #194156

    Purpose

    Engineered PcrA_M6 helicase to support isothermal amplification method SHARP

    Depositor

    Taekjip Ha

    Article

    Gavrilov et al Nat Commun. 2022 Oct 23;13(1):6312. doi:

    Insert

    Engineered helicase PcrA_M6
    

    Use

    Tags

    6x His tag and FLAG tag

    Expression

    Bacterial

    Mutation

    H93A, C96A, N187C, C247A, L384V, L409C

    Promoter

    Available since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-ZNF598-TEV-3xFLAG

    Plasmid

    #105690

    Purpose

    Expresses C terminally 3xFLAG tagged ZNF598 protein

    Depositor

    Ramanujan Hegde

    Article

    Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

    Insert

    ZNF598 (ZNF598 Human)
    

    Use

    Tags

    TEV-3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV (from vector)

    Available since

    Feb. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. lenti-tmpknot-epegRNA-puro backbone

    Plasmid

    #214089

    Purpose

    Lentiviral backbone plasmid to insert epegRNAs (mpknot) of interest. The cloning site is BsmbI.

    Depositor

    Ting Zhou

    Article

    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6, EF1a

    Available since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCPP6534

    Plasmid

    #128735

    Purpose

    Gateway destination pUC18R6KT-Tn7T-PavrPto-GW-Cya-Km vector for integration into DC3000D36E genome glmS-linked attTn7 site any effector gene, expressed from avrPto promoter and with C-terminal HA tag

    Depositor

    Alan Collmer

    Article

    Wei et al Cell Rep. 2018 May 8;23(6):1630-1638. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLKO.1-shCtnnd2.1-GFP

    Plasmid

    #209095

    Purpose

    GFP expressing shRNA targeting Ctnnd2 N-terminus

    Depositor

    Cagla Eroglu

    Article

    Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

    Insert

    shCtnnd2.1
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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