We narrowed to 13,360 results for: Mpl;

1. pAPM-D4 miR30-DNMT3a ts3

   Plasmid

   #115867

   Purpose

   DNMT3a knockdown

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

   Insert

   DNMT3a shRNA (DNMT3A Human)
   

   Use

   Lentiviral

   Promoter

   SFFV

   Available Since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA1 Flag ATXN1[85Q]-S776A

   Plasmid

   #33240

   Depositor

   Huda Zoghbi

   Article

   Lam et al Cell. 2006 Dec 29;127(7):1335-47.

   Insert

   Ataxin-1 (ATXN1 Human)
   

   Tags

   flag

   Expression

   Mammalian

   Mutation

   insert contains a stretch of 85 Glutamines and no…

   Promoter

   CMV

   Available Since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pPPAT-11AA-2XStrep

   Plasmid

   #125680

   Purpose

   expresses human PPAT protein, with first 11 residues removed, with a C-terminal 2XStrep affinity tag

   Depositor

   Stephen Benkovic

   Article

   Liu et al J Proteome Res. 2019 May 3;18(5):2078-20

   Insert

   PPAT (PPAT Human)
   

   Tags

   2X Strep-Tag II

   Expression

   Mammalian

   Mutation

   the first eleven residues have been removed

   Promoter

   CMV

   Available Since

   May 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pETDuet-1-6xHis-TEV-Nsp8

   Plasmid

   #201022

   Purpose

   Bacterial expression of codon optimized SARS-CoV-2 nsp8 tagged with an N-terminus His-tag followed by a TEV protease cleavage site.

   Depositor

   Sharon Rozovsky

   Article

   Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

   Insert

   non-structural protein 8 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
   

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Gene insert is codon optimized for Ecoli.

   Promoter

   T7/LacO

   Available Since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5-FRT-TO-Nsp7-HF

   Plasmid

   #157690

   Purpose

   mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp7 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   Nsp7-HF (ORF1ab SARS-CoV-2)
   

   Tags

   His8-Flag

   Expression

   Mammalian

   Mutation

   codon optimized

   Available Since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pmiRGLO-SERINC2-202

   Plasmid

   #117364

   Purpose

   Dual luciferase assay for miRNA-targeted UTR

   Depositor

   Volker Busskamp

   Article

   Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

   Insert

   3' UTR SERINC2-202
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Promoter

   Murine PGK-1

   Available Since

   Dec. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX-6P-1-INTS3-N

   Plasmid

   #128416

   Purpose

   Express INTS3 N-termini (1-513 aa) in E. coli with a GST tag (N-terminal)

   Depositor

   Yuliang Wu

   Article

   Vidhyasagar et al Biochem J. 2018 Jan 2;475(1):45-60. doi:

   Insert

   INTS3 (INTS3 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   N-terminal region (1–513 aa)

   Promoter

   tac promoter

   Available Since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. [pSK496] pLJC5 HA-Depdc5(AB)

   Plasmid

   #109346

   Purpose

   Lenti-viral expression of HA-tagged Depdc5 mutant AB

   Depositor

   David Sabatini, Kuang Shen

   Article

   Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

   Insert

   Depdc5 (DEPDC5 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Mutation

   DIYGD(185-189)GSGSG;EQPLH(371-375)GSGSG

   Available Since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAPM-D4 miR30-AGO3 ts3

   Plasmid

   #115858

   Purpose

   AGO3 knockdown

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

   Insert

   AGO3 shRNA (AGO3 Human)
   

   Use

   Lentiviral

   Promoter

   SFFV

   Available Since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAPM-D4 miR30-AGO3 ts2

    Plasmid

    #115857

    Purpose

    AGO3 knockdown

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    AGO3 shRNA (AGO3 Human)
    

    Use

    Lentiviral

    Promoter

    SFFV

    Available Since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENTR221-TAZ CA

    Plasmid

    #79504

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Human TAZ S89A mutant CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    WWTR1 (WWTR1 Human)
    

    Use

    Multisite gateway entry clone

    Tags

    3xFLAG

    Mutation

    S89A

    Promoter

    no Promoter

    Available Since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBScMXT + mSlo1 MBr5/3

    Plasmid

    #16149

    Depositor

    Larry Salkoff

    Article

    Butler et al Science. 1993 Jul 9. 261(5118):221-4.

    Insert

    Mus musculus potassium large conductance calcium-activated channel (Kcnma1 Mouse brain)
    

    Use

    Xenopus oocyte expression

    Mutation

    N/A

    Available Since

    Jan. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

13. pEBTetBl-CLIP-Centrobin

    Plasmid

    #136878

    Purpose

    Mammalian expression of the centrosomal protein Centrombin N-terminally fused to CLIP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    CLIP-Centrobin (CNTROB Human, Synthetic)
    

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Promoter

    CMV-TetO2

    Available Since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEBTetBl-CLIP-CP110

    Plasmid

    #136851

    Purpose

    Mammalian expression of the centrosomal protein Cep110 N-terminally fused to CLIP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    CLIP-CP110 (CCP110 Human, Synthetic)
    

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Promoter

    CMV-TetO2

    Available Since

    April 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGBT9-Hook2

    Plasmid

    #198524

    Purpose

    Expression of GAL4 DNA-binding domain (BD)-Hook2 fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

    Insert

    Hook2 (HOOK2 Human)
    

    Tags

    GAL4-DNA binding domain fragment

    Expression

    Yeast

    Mutation

    His488Gln substitution

    Promoter

    ADH1

    Available Since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA5-FRT-TO-FH-Nsp8

    Plasmid

    #157695

    Purpose

    mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    FH-Nsp8 (ORF1ab SARS-CoV-2)
    

    Tags

    Flag-His8

    Expression

    Mammalian

    Mutation

    codon optimized

    Available Since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pNTKV- Utf1-KI-3'UTR-2A-tdTomato-RBGpA

    Plasmid

    #128834

    Purpose

    Knock-in of tdTomato into mouse Utf1 locus

    Depositor

    Yossi Buganim

    Article

    Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

    Insert

    tdTomato into Utf1 locus (Utf1 Mouse)
    

    Use

    Mouse Targeting

    Available Since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pmiRGLO-BAX-201

    Plasmid

    #117332

    Purpose

    Dual luciferase assay for miRNA-targeted UTR

    Depositor

    Volker Busskamp

    Article

    Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

    Insert

    3' UTR BAX-201
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Promoter

    Murine PGK-1

    Available Since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. p.UTA.2.0 miR-27a-3p 3 perfect copies.

    Plasmid

    #82439

    Purpose

    Dual Fluorescent endogenous miRNA sensor. Perfect complementary region of hsa-miR-27a-3p in three copies

    Depositor

    Jens Gruber

    Article

    Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

    Insert

    hsa-miR-27a-3p (MIR27A Human)
    

    Mutation

    Three copies hsa-miR-27a-3p Complementary region …

    Available Since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAC8_MF-PH-Flag-Nter (VE5627)

    Plasmid

    #139773

    Purpose

    Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an N-ter Flag tag under the pH promoter.

    Depositor

    Arnaud Poterszman

    Article

    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

    Insert

    N-terminal Flag tag
    

    Tags

    Flag

    Promoter

    PH

    Available Since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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