We narrowed to 8,455 results for: gnal

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pCDB367

Plasmid

#110277

Purpose

gHEEE_02 with a N-terminal 10-His DsbC TEV-peptide fusion

Depositor

Christopher Bahl

Article

Buchko et al Protein Sci. 2018 Aug 26. doi: 10.1002/p

Insert

gHEEE_02

Tags

10-His, Tev recognition peptide, and mature DsbC …

Expression

Bacterial

Promoter

T7

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc-mCE NLS

Plasmid

#82465

Purpose

Expresses myc tag and mRNA capping enzyme without Nuclear Localization Sequence (NLS) from C terminus

Depositor

Daniel Schoenberg

Article

Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

Insert

Capping Enzyme (Rngtt Mouse)

Tags

Myc

Expression

Mammalian

Mutation

deleted amino acids Nuclear Localization Signal

Promoter

CMV

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc NES-mCE

Plasmid

#82463

Purpose

Expression of myc epitope and murine mRNA Capping Enzyme with Terminal nuclear export signal in mammalian cells

Depositor

Daniel Schoenberg

Article

Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

Insert

NES mRNA capping enzyme (Rngtt HIV, Mouse)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 STAT1

Plasmid

#67116

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

STAT1 (STAT1 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pYNL-C1_PTS1

Plasmid

#65699

Purpose

Expresses YNL-PTS1 in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

peroxisomal targeting signal 1 (tripeptide SKL)

Use

Luciferase

Tags

YNL

Expression

Mammalian

Promoter

CMV

Available Since

July 20, 2015

Availability

Academic Institutions and Nonprofits only

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pCNL-C1_PTS1

Plasmid

#65700

Purpose

Expresses CNL-PTS1 in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

peroxisomal targeting signal 1 (tripeptide SKL)

Use

Luciferase

Tags

CNL

Expression

Mammalian

Promoter

CMV

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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pONL-C1_PTS1

Plasmid

#65701

Purpose

Expresses ONL-PTS1 in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

peroxisomal targeting signal 1 (tripeptide SKL)

Use

Luciferase

Tags

ONL

Expression

Mammalian

Promoter

CMV

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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pcdna3 EE Jip1b

Plasmid

#52127

Purpose

a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

Depositor

Roger Davis

Article

Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

Insert

JNK interacting protein-1b (JIP-1b) (Mapk8ip1 Mouse)

Tags

EE

Expression

Mammalian

Available Since

April 18, 2014

Availability

Academic Institutions and Nonprofits only
pET21-MRPL13

Plasmid

#31344

Depositor

Linda Spremulli

Article

Emdadul Haque et al Mitochondrion. 2008 Jun . 8(3):254-61.

Insert

mitochondrial ribosomal protein L13 mature form (MRPL13 Human)

Tags

His

Expression

Bacterial

Mutation

Mature form after removal of the predicted mitoch…

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
pCeSR beta deltaN

Plasmid

#13728

Depositor

Thomas Schwartz

Article

Schwartz et al Proc Natl Acad Sci U S A. 2006 May 2. 10

Insert

Signal recognition particle receptor β subunit (R186.3 Nematode)

Tags

6xHis

Expression

Bacterial

Mutation

expresses residues 38-240 of C. elegans SRβ with …

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

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pScSR beta deltaN Flag

Plasmid

#13729

Depositor

Thomas Schwartz

Article

Schwartz et al Proc Natl Acad Sci U S A. 2006 May 2. 10

Insert

Signal recognition particle receptor β subunit (SRP102 Budding Yeast)

Tags

6xHis and Flag

Expression

Bacterial

Mutation

expresses residues 31-244 of S. cerevisiae SRβ wi…

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mCherry-PKCsub-RLuc8N-NES

Plasmid

#138213

Purpose

Encodes substrate fragment of luminescence-based bimolecular PKC activity reporter (LumCKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.

Depositor

Jin Zhang

Article

Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

Insert

mCherry-PKCsub-RLuc8N-NES

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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anti-FLAG M2 heavy chain

Plasmid

#175359

Purpose

Mammalian expression vector for over-expression of anti-FLAG M2 heavy chain (C-terminal His8)

Depositor

Joost Snijder

Article

Peng et al J Proteome Res. 2021 Jul 2;20(7):3559-35

Insert

anti-FLAG M2 heavy chain

Tags

His8 tag and N-terminal secretion signal peptide …

Expression

Mammalian

Mutation

L51I (see paper)

Promoter

CMV

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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m6A-Tracer-NES

Plasmid

#159607

Purpose

Constitutive transient mammalian expression of m6A-Tracer protein (Kind et al. 2013) with a C-terminal HIV-1 Rev Nuclear Export Signal to reduce background due to nonspecific DNA interactions.

Depositor

Aaron Streets

Article

Altemose et al Cell Syst. 2020 Oct 21;11(4):354-366.e9.

Insert

m6A-Tracer-NES

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-FLEX-NEPLDCV-P2A-mRUBY3-WPRE

Plasmid

#207664

Purpose

Neuropeptide degradation

Depositor

Sung Han

Article

Kim et al bioRxiv 2023.01.19.524797

Insert

NEP (MME Human)

Use

AAV and Cre/Lox

Tags

mRUBY3

Mutation

Addition of signaling peptide (POMC) on the N-ter…

Promoter

hSynapsin

Available Since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pPIC9K_A2AR_316_V229C_R291A

Plasmid

#246917

Purpose

Express A2AR mutants in Pichia Pastoris

Depositor

Libin Ye

Article

Wang et al Nat Commun. 2023 Mar 10;14(1):1325. doi:

Insert

Adenosine A2A receptor (ADORA2A Human)

Tags

10xHis and alpha-factor secretion signal, FLAG

Expression

Yeast

Mutation

truncated to aa 1-316, V229C, R291A

Promoter

AOX1

Available Since

Nov. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag MKK3b(Glu)

Plasmid

#50449

Purpose

activates p38 in the signaling pathway

Depositor

Roger Davis

Article

Enslen et al EMBO J. 2000 Mar 15. 19(6):1301-11.

Insert

MKK3b (MAP2K3 Human)

Tags

Flag

Expression

Mammalian

Mutation

changed Serine 218 to Glutamic Acid, changed Thre…

Promoter

CMV

Available Since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only
pTDpelB-C_sfYFPTwinStrep

Plasmid

#45944

Purpose

expresses P.putida codon optimized PelB-sfYFP-Twin-Strep fusion protein for periplasmic translocation via PelB signal sequence

Depositor

Thorben Dammeyer

Article

Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

Insert

synthetic sfYFP (codon usage adapted to P.putida KT2440)

Tags

PelB export sequence and Twin-Strep-tag

Expression

Bacterial

Promoter

lacIq/Ptrc

Available Since

Sept. 18, 2013

Availability

Academic Institutions and Nonprofits only

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pTDpelB-NTwinStrep

Plasmid

#45940

Purpose

use to create N-terminal PelB-Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal PelB-Twin-Strep-tag and C-terminal 6x His-tag for periplasmic translocation via PelB signal sequence

Depositor

Thorben Dammeyer

Article

Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

Type

Empty backbone

Tags

6xHis, PelB, and Twin-Strep

Expression

Bacterial

Promoter

Trc (lactose/IPTG inducible)

Available Since

Sept. 18, 2013

Availability

Academic Institutions and Nonprofits only

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pEX-CMV-SP-YFP-STM2(15-746)

Plasmid

#18862

Depositor

Tobias Meyer

Article

Brandman et al Cell. 2007 Dec 28. 131(7):1327-39.

Insert

Stromal interaction molecule 2 (STIM2 Human)

Tags

YFP

Expression

Mammalian

Mutation

YFP inserted between signal peptide(SP) of STIM1 …

Available Since

March 24, 2009

Availability

Academic Institutions and Nonprofits only

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