165,307 results

1. Cas9-GFP_sg_mAMPKa1

   Plasmid

   #79004

   Purpose

   Expresses WT Cas9 and GFP, along with a sgRNA to mouse AMPK alpha 1.

   Depositor

   Reuben Shaw

   Article

   Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

   Insert

   Prkaa1 (Prkaa1 Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   June 22, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pJYS1Peftu

   Plasmid

   #85546

   Purpose

   Constitutive expression of FnCpf1 and recT in C.glutamitum, etfu promoter

   Depositor

   Sheng Yang

   Article

   Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

   Inserts

   Cpf1
   recT
   

   Use

   CRISPR; Shuttle vector corynebacterium glutamicum…

   Expression

   Bacterial

   Promoter

   etfu and unknown

   Available Since

   May 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pREP3-TEV-NLS

   Plasmid

   #21146

   Depositor

   Stephen Kearsey

   Article

   Yang et al BMC Mol Biol. 2005 Jun 7;6:13. doi: 10.1

   Insert

   TEV protease (TEVgp1 tobacco etch virus)
   

   Use

   S. pombe

   Tags

   2xNLS, NLS, and myc

   Expression

   Yeast

   Available Since

   July 6, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. p2R3a-GUS-OcsT

   Plasmid

   #71267

   Purpose

   Entry clone containing the GUS enzyme. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway system

   Depositor

   Ari Pekka Mähönen

   Article

   Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

   Insert

   Beta-glucuronidase
   

   Use

   Gateway

   Tags

   2xGly linker and octaline synthase terminator

   Available Since

   Dec. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC18-mini-Tn7T-Gm-lacZ

   Plasmid

   #63120

   Purpose

   mini-Tn7 lacZ transcriptional fusion vector

   Depositor

   Herbert Schweizer

   Article

   Choi et al Nat Methods. 2005 Jun;2(6):443-8.

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   May 17, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pMRE-Tn5-161

   Plasmid

   #118541

   Purpose

   miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   mTurquoise2
   

   Use

   Minitn5 delivery vector

   Expression

   Bacterial

   Available Since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR EGFR-CD3ε-FusionRed

   Plasmid

   #188626

   Purpose

   Human EGFR labeled with CD3ε ITAMs for monitoring with CD3ε/ZtSH2 pYtag biosensor

   Depositor

   Jared Toettcher

   Article

   Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

   Insert

   EGFR (EGFR Synthetic, Human)
   

   Use

   Lentiviral

   Tags

   CD3ε-FusionRed

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEntr_Myc-BirA-G3-ER

   Plasmid

   #173814

   Purpose

   Promiscuous biotin ligase (BirA-G3) targeted to ER lumen in Gateway entry vector. N-terminal secretion signal from Drosophila BiP. C-terminal KDEL. Myc tag inserted after sig. seq. cleavage site

   Depositor

   Norbert Perrimon

   Article

   Droujinine et al Nat Commun. 2021 Apr 22;12(1):2382. doi:

   Insert

   Myc-BirA-G3-ER
   

   Use

   Gateway shuttle vector

   Tags

   Myc

   Available Since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTK_Sox10_enh_84_Citrine

   Plasmid

   #130584

   Purpose

   enhancer reporter

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

   Insert

   Sox10_enh_84
   

   Use

   Enhancer reporter

   Promoter

   thymidine kinase minimal promoter

   Available Since

   Nov. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. hsDCX-EGFP-pHAT

    Plasmid

    #83918

    Purpose

    human Doublecortin, N-terminal 6-His tag, C-terminal EGFP-Strep-tag II

    Depositor

    Gary Brouhard

    Article

    Bechstedt et al Dev Cell. 2012 Jul 17;23(1):181-92. doi:

    Insert

    Doublecortin isoform b (DCX NM_178152.2, Human)
    

    Tags

    6-His and EGFP+Strep-tag II

    Expression

    Bacterial

    Available Since

    Dec. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-eGFP-pA

    Plasmid

    #107281

    Purpose

    donor vector backbone with constitutive eGFP

    Depositor

    Knut Woltjen

    Article

    Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

    Insert

    GFP
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPI356

    Plasmid

    #207449

    Purpose

    Extrachromosomal expression vector PkgE-PH-GFP and PTEN-mCherry

    Depositor

    Rob Kay

    Article

    Lutton et al Curr Biol. 2023 Aug 7;33(15):3083-3096.e

    Inserts

    PkgE
    PTEN
    

    Use

    Unspecified

    Tags

    GFP and mCherry

    Mutation

    PH domain only

    Available Since

    Dec. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDM1383

    Plasmid

    #207423

    Purpose

    Extrachromosomal expression HSPC300-GFP and mCherry-Hs Raf1 (1–134 RBD)

    Depositor

    Rob Kay

    Article

    Veltman et al Elife. 2016 Dec 13;5:e20085. doi: 10.755

    Inserts

    HSPC300
    Raf1 (RAF1 Human)
    

    Use

    Unspecified

    Tags

    GFP and mCherry

    Mutation

    aa 1–134 (RBD) only

    Available Since

    Nov. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. XX01CAPN1A-c001

    Plasmid

    #175491

    Purpose

    Protein expression in bacterial cells. Entire CDS, M1-A714 (CAPN1); EF hand 1-5, H86-S268 (CAPNS1). CAPN1 can be biotinylated. Codon optimised.

    Depositor

    Opher Gileadi

    Insert

    CAPN1 / CAPNS1 (CAPN1 Human)
    

    Tags

    AviTag and His6-TEV

    Expression

    Bacterial

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDIRECT_22A

    Plasmid

    #91133

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:npt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: 35S:AtCas9 + AtU6:gRNA
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMSCVpuro-mTagBFP2

    Plasmid

    #96935

    Purpose

    Retroviral plasmid containing mTagBFP2 for labeling of cells

    Depositor

    Lee Grimes

    Article

    Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

    Insert

    Monomeric Tag Blue Fluorescent Protein
    

    Expression

    Mammalian

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. lenti EF1a-FLAG-dCas9-VPR

    Plasmid

    #114195

    Purpose

    Lentivirus compatible dCas9-VPR construct driven by the EF1a promoter

    Depositor

    Jeremy Day

    Article

    Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

    Insert

    dCas9-VPR
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. mLama1 AAV sgRNA 1, 2, 5

    Plasmid

    #135339

    Purpose

    Expression plasmid of VP64-SadCas9 sgRNA 1, 2 and 3

    Depositor

    Ronald Cohn

    Article

    Kemaladewi et al Nature. 2019 Aug;572(7767):125-130. doi:

    Insert

    Lama1 VP64-dCas9 sgRNA Guides
    

    Use

    AAV

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDH-CMV-human MYCN-EF1-mRFP

    Plasmid

    #193708

    Purpose

    Lentiviral expression of human MYCN

    Depositor

    Martine Roussel

    Insert

    MYCN (MYCN Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA4/TO mTRPM7

    Plasmid

    #45482

    Depositor

    Andrew Scharenberg

    Article

    Nadler et al Nature. 2001 May 31;411(6837):590-5.

    Insert

    TRPM7 (Trpm7 Mouse)
    

    Use

    Tetracycline inducible

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    Tetracycline-controlled CMV

    Available Since

    May 21, 2013

    Availability

    Academic Institutions and Nonprofits only

21. HA-Beclin 1

    Plasmid

    #86748

    Purpose

    N-terminal HA-tagged protein expression in mammalian cells

    Depositor

    Do-Hyung Kim

    Article

    Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

    Insert

    human Beclin 1 (BECN1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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22. pUBC_stdMCP_serinemod_E488QADAR_p2A_yGFP

    Plasmid

    #154787

    Purpose

    Expresses synonymized MS2 coat protein dimer (MCP) fused to hyperactive ADAR catalytic domain

    Depositor

    Robert Singer

    Article

    Biswas et al iScience. 2020 Jun 28;23(7):101318. doi:

    Insert

    Adenosine deaminse (Adar Synthetic, Fly)
    

    Use

    Lentiviral

    Tags

    GFP, V5, and stdMCP

    Expression

    Mammalian

    Mutation

    Synonymized serine to prevent autoediting of ADAR…

    Promoter

    UBC

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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23. pcDNA3-EGFP-Rac1-Q61L

    Plasmid

    #12981

    Depositor

    Gary Bokoch

    Article

    Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

    Insert

    Rac1 constitutively active (RAC1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Q61L Constitutively active

    Available Since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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24. pcDNA3-FLAG-HA-hYTHDC1

    Plasmid

    #85167

    Purpose

    Expression of FLAG-HA-tagged human YTHDC1

    Depositor

    Samie Jaffrey

    Article

    Patil et al Nature. 2016 Sep 7;537(7620):369-373. do

    Insert

    YTHDC1 (YTHDC1 Human)
    

    Tags

    Flag and Ha

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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25. pVE13300

    Plasmid

    #137715

    Purpose

    Expresses impLbCas12a in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576

    Insert

    impLbCas12a
    

    Tags

    3xHA, NLS, and SV40 NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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26. pT7-7 asyn A53T

    Plasmid

    #105727

    Purpose

    Bacterial expression of mutant human alpha synuclein

    Depositor

    Hilal Lashuel

    Article

    Rospigliosi et al J Mol Biol. 2009 May 22;388(5):1022-32.

    Insert

    alpha-synuclein A53T (SNCA Human)
    

    Expression

    Bacterial

    Mutation

    A53T

    Promoter

    T7

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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27. LeGO-iG

    Plasmid

    #27358

    Depositor

    Boris Fehse

    Article

    Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

    Insert

    U6 promoter, SFFV promoter, IRES-eGFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    April 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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28. mCherry-Vimentin-7

    Plasmid

    #55156

    Purpose

    Localization: Intermediate Filaments, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Article

    Shaner et al J Cell Sci. 2007 Dec 15;120(Pt 24):4247-

    Insert

    Vimentin (VIM Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

29. pRSF-G1mCNPRS

    Plasmid

    #174718

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of L-3-(2-cyano-4-pyridyl)alanine (mCNP), into proteins in E. coli in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Abdelkader et al Angew Chem Int Ed Engl. 2022 Jan 31. doi

    Insert

    tRNA synthetase
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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30. L1-neo-TET

    Plasmid

    #51284

    Purpose

    expresses codon optimized human L1 retrotransposon driven by CMV promoter and tagged with the self splicing intron neo cassette

    Depositor

    Astrid Roy-Engel

    Article

    Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:

    Insert

    L1RE1 (L1RE1 Human)
    

    Tags

    neoTET cassette with a tetrahymena self-splicing …

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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