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pcDNA 3.2-FUS-1-373aa-V5

Plasmid

#29611

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-1-373aa (FUS Human)

Tags

V5

Expression

Mammalian

Mutation

Contains FUS aa# 1-373

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only
pLPC-Progerin-CS

Plasmid

#69064

Purpose

encodes Progerin with a substitution of cysteine in CaaX-motif by a serine

Depositor

Gerardo Ferbeyre

Article

Moiseeva et al Cell Cycle. 2015 Aug 3;14(15):2408-21. d

Insert

Progerin (LMNA Human)

Use

Retroviral

Expression

Mammalian

Mutation

Progerin with substitution of cysteine in CaaX-mo…

Promoter

CMV

Available Since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only
pEF1α-mouse-full-length-CHMP3-HA

Plasmid

#154176

Purpose

expresses mouse CHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

CHMP3 (Chmp3 Mouse)

Tags

HA

Expression

Mammalian

Promoter

EF1alpha

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pNK3203

Plasmid

#219749

Purpose

DVK_AF CIDAR vector encoding hispidin-synthase from Mycena citricolor under control of CMV promoter, for mammalian expression

Depositor

Alexander Mishin

Article

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

pCMV - mcitHispS - tSV40

Expression

Mammalian

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR v2 puro hGSDME gRNA2

Plasmid

#223520

Purpose

Knocking out hGSDME in human cells

Depositor

Judy Lieberman

Article

Zhang et al Nature. 2020 Mar;579(7799):415-420. doi:

Insert

GSDME (GSDME Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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416Gal-FUS-R521G-YFP

Plasmid

#29623

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS (FUS Human)

Tags

YFP

Expression

Yeast

Mutation

R521G

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
KAT6B-luciferase

Plasmid

#113353

Purpose

evaluate FOXA1 DIV motif enhancer activity near 50 kb of KAT6B gene

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

KAT6B-enhancer (KAT6B Human)

Use

Luciferase

Available Since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_mGFP-FGFR3-K650E

Plasmid

#191757

Purpose

Expression of N-terminally mGFP labelled mutated FGFR3 (isoform IIIc)-K650E mutation (associated with TD2; target mutation: c.1948A>G; silent mutation added on purpose: c.1938C>T)

Depositor

Irene Tiemann-Boege

Article

Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi

Insert

mGFP-FGFR3IIIc

Tags

mGFP

Expression

Mammalian

Mutation

c.1948A>G (p.K650E) + silent mutation c.1938C&…

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pBMN mCherry-p62(W340A/ΔUBD)

Plasmid

#119688

Purpose

For mammalian expression of LIR mutant p62 without its UBD

Depositor

Michael Lazarou

Article

Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

Insert

p62 (SQSTM1 Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

W340A & UBD deletion (1-385)

Available Since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pTE4495

Plasmid

#80338

Purpose

mammalian expression MbCpf1 nuclease and Mb crRNA

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Inserts

Mb crRNA
MbCpf1

Use

CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Promoter

CMV and human U6

Available Since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pAF197

Plasmid

#135930

Purpose

All-in-one Sleeping Beauty vector for Expression for Tasmanian devil CTLA4-Fc-mCherry fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. CTLA4).

Depositor

Andrew S. Flies

Article

Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab

Insert

CTLA4 fused to IgG Fc and to mCherry

Use

Transposon

Tags

mCherry

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/CIP2A(1-905) R522D V5 His

Plasmid

#119289

Purpose

CIP2A R522D V5 overexpression, CIP2A dimer defective mutant

Depositor

Jukka Westermarck

Article

Wang et al EMBO Rep. 2017 Mar;18(3):437-450. doi: 1

Insert

KIAA1524 (CIP2A Human)

Tags

V5 His

Expression

Mammalian

Mutation

R522D

Promoter

T7

Available Since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

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416GPD-FUS-YFP

Plasmid

#29594

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS (FUS Human)

Tags

YFP

Expression

Yeast

Available Since

July 18, 2011

Availability

Academic Institutions and Nonprofits only
pGL3-ACSL4-FL

Plasmid

#202515

Purpose

Expressing luciferase driven by human ACSL4 promoter (bases -1 to -2844 with respect to exon1 start site)

Depositor

Ming Chen

Article

Wang et al J Clin Invest. 2023 Mar 16:e166647. doi:

Insert

ACSL4 promoter (partial) (ACSL4 Human)

Use

Luciferase

Expression

Mammalian

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pMEXneo-TrkC

Plasmid

#190202

Purpose

To express the Human TrkC receptor under the Moloney mouse sarcoma virus LTR.

Depositor

Pantelis Tsoulfas

Article

Urfer et al J Biol Chem. 1998 Mar 6;273(10):5829-40.

Insert

TrkC (NTRK3 Human)

Use

Eucaryotic

Expression

Mammalian

Promoter

Moloney mouse sarcoma virus LTR

Available Since

June 7, 2023

Availability

Academic Institutions and Nonprofits only

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GST-TEV-FUS-267-526aa

Plasmid

#29630

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-267-526aa (FUS Human)

Tags

GST and TEV

Expression

Bacterial

Mutation

Contains FUS aa# 267-526

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only
GST-TEV-FUS-1-373aa

Plasmid

#29631

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-1-373aa (FUS Human)

Tags

GST and TEV

Expression

Bacterial

Mutation

Contains FUS aa# 1-373

Available Since

June 6, 2011

Availability

Academic Institutions and Nonprofits only
423Gal-FUS-YFP

Plasmid

#29615

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS (FUS Human)

Tags

YFP

Expression

Yeast

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3.1_mGFP-FGFR3-G380R

Plasmid

#191755

Purpose

Expression of N-terminally mGFP labelled mutated FGFR3 (isoform IIIc)-G380R mutation (associated with ACH; target mutation: c.1138G>A; silent mutation added on purpose: c.1131C>T)

Depositor

Irene Tiemann-Boege

Article

Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi

Insert

mGFP-FGFR3IIIc

Tags

mGFP

Expression

Mammalian

Mutation

c.1138G>A (G380R) + c.1131C>T (silent mutat…

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pTE4999

Plasmid

#107555

Purpose

Expresses human codon optimized inactive FnCpf1(dead1) in mammalian cells.

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Insert

hFnCpf1(dead1)

Use

CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Mutation

D917A

Promoter

CMV

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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