165,550 results

1. pcDNA3.2/v5-DEST hGlut4

   Plasmid

   #18087

   Depositor

   Wolf Frommer

   Article

   Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

   Insert

   Glut4 (SLC2A4 Human)
   

   Expression

   Mammalian

   Available Since

   Feb. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. NYESO beta/alpha into TRAC HDRT Source (pTR 169)

   Plasmid

   #112021

   Purpose

   DNA sequence source for amplifying an HDR template to replace endogenous human TCR with 1G4 NYESO TCR at TCR-alpha

   Depositor

   Alexander Marson

   Article

   Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

   Insert

   NYESO beta/alpha into TRAC HDRT
   

   Use

   CRISPR and Synthetic Biology

   Available Since

   Sept. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1_3xFlagEzh2 WT

   Plasmid

   #173717

   Purpose

   WT EZH2 expression plasmid with 3XFLAG tag

   Depositor

   Thomas Cech

   Article

   Long et al Nat Genet. 2020 Sep;52(9):931-938. doi:

   Insert

   EZH2 (EZH2 Human)
   

   Tags

   3XFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. N8his-GFPenhancer-GGGGS4-LaG16

   Plasmid

   #140442

   Purpose

   We designed a flexible linker to connect two types of anti GFP nanobodies and used this fusion nanobody to purify GFP tagged protein.

   Depositor

   Motoyuki Hattori

   Article

   Zhang et al Sci Rep. 2020 Apr 10;10(1):6239. doi: 10

   Insert

   GFPenhancer-GGGGS4-LaG16
   

   Tags

   8 histidine tag

   Expression

   Bacterial

   Promoter

   T7 promotor

   Available Since

   May 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC18T-mini-Tn7T-Gm-Pc-mNeonGreen

   Plasmid

   #205022

   Purpose

   mini-Tn7 vector for insertion of mNeonGreen (codon optimized for P. fluorescens) at bacterial attTn7 site

   Depositor

   Rosemarie Wilton

   Article

   Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

   Insert

   mNeonGreen
   

   Expression

   Bacterial

   Mutation

   mNeonGreen is codon optimized for expression in P…

   Promoter

   Pc

   Available Since

   Sept. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-HA-JMJD2B

   Plasmid

   #24181

   Purpose

   Mammalian expression of human JMJD2B with HA tag

   Depositor

   Kristian Helin

   Article

   Cloos et al Nature. 2006 Jul 20. 442(7100):307-11.

   Insert

   JMJD2B (KDM4B Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Jan. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCW57.1 blast tBID-flag

   Plasmid

   #211533

   Purpose

   Expresses truncated BID upon doxycycline addition to trigger apoptosis

   Depositor

   Richard Possemato

   Article

   Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

   Insert

   tBID-flag (BID Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Truncated (CASP8) form (p15)

   Available Since

   Jan. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFA6a-mCherry-sfGFP-kanMX (pMaM17)

   Plasmid

   #173452

   Purpose

   Contains mCherry-sfGFP tandem fluorescent protein timer

   Depositor

   Anton Khmelinskii

   Article

   Khmelinskii et al Nat Biotechnol. 2012 Jun 24;30(7):708-14

   Insert

   mCherry-sfGFP-ADH1ter
   

   Expression

   Yeast

   Promoter

   no promoter

   Available Since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMK025

   Plasmid

   #236572

   Purpose

   Cloning vector for PaqCI Golden Gate assembly of ISYmu1 guides into ISYmu1 plant expression vector

   Depositor

   Steven Jacobsen

   Article

   Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   May 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPD304 VPR-ZF43

    Plasmid

    #138840

    Purpose

    Constitutive expression of VPR-ZF43 under the CMV promoter.

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    VPR-ZF43
    

    Use

    Synthetic Biology

    Tags

    3xFlag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMK290 (mAID-mClover-Hygro)

    Plasmid

    #72828

    Purpose

    C-terminal tagging with mAID-mClover using CRISPR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mAID-mClover-Hygro
    

    Use

    CRISPR

    Tags

    mAID-mClover

    Expression

    Mammalian

    Available Since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. miniTurbo-V5_pRS415

    Plasmid

    #107168

    Purpose

    expresses V5-tagged miniTurbo in the yeast cytosol

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    miniTurbo (BirA mutant)
    

    Expression

    Yeast

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA5/FRT/TO_TOMM20-SNAP-tag2-T2A-EGFP

    Plasmid

    #226516

    Purpose

    Mamalian cell expression of SNAP-tag2 on mitochondrial surface and cytosolic EGFP

    Depositor

    Kai Johnsson

    Article

    Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

    Insert

    TOMM20-SNAP-tag2-T2A-EGFP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMMP-TVA800

    Plasmid

    #15778

    Depositor

    Edward Callaway

    Article

    Wickersham et al Neuron. 2007 Mar 1. 53(5):639-47.

    Insert

    TV A800
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Oct. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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15. U6-5'+3' MS2-CircRNA

    Plasmid

    #213752

    Purpose

    For circular RNA-mediated prime editor using U6-5'+3' MS2-CircRNA in HEK293T cells

    Depositor

    Caixia Gao

    Article

    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Insert

    5' ribozyme, 5' ligation sequences, 5' MS2, 3' MS2, 3' ligation sequences, 3' ribozyme
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAR18_StrepI_mFASm_H8_pET22b

    Plasmid

    #122847

    Purpose

    Expresses murine type I fatty acid synthase (FASN) in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.

    Depositor

    Martin Grininger

    Article

    Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

    Insert

    mouse FASN/ mouse fatty acid synthase (Fasn Mouse)
    

    Tags

    His8 tag and Twin-Strep tag

    Expression

    Bacterial

    Mutation

    wildtype

    Promoter

    T7 promoter

    Available Since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGWB505

    Plasmid

    #74847

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with sGFP (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-hPPP3CA-FLAG

    Plasmid

    #179134

    Purpose

    Expression vector of human protein phosphatase 3, catalytic subunit, alpha isoform (PPP3CA) tagged with FLAG at C-terminus, CAG promoter, rabbit globin poly(A) signal.

    Depositor

    Masahito Ikawa

    Article

    Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

    Insert

    protein phosphatase 3, catalytic subunit, alpha isoform (PPP3CA Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRedF

    Plasmid

    #118057

    Purpose

    GBPart - RedF Luciferase CDS

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Insert

    Red Firefly Luciferase CDS
    

    Use

    Synthetic Biology; Domestication of dna parts for…

    Mutation

    Red mutant S286Y

    Available Since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBABE

    Plasmid

    #21836

    Depositor

    David Ron

    Insert

    EGFP
    

    Use

    Retroviral

    Tags

    farnysylation signal

    Expression

    Mammalian

    Available Since

    Sept. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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