We narrowed to 8,324 results for: gnal

1. pTriEx-mCherry-LINXa3-nano

   Plasmid

   #73616

   Purpose

   Light-induced nuclear export switch fusion to nano for microscopy in mammalian cells.

   Depositor

   Brian Kuhlman

   Article

   Yumerefendi et al Nat Chem Biol. 2016 Jun;12(6):399-401. d

   Insert

   LINXa3-nano
   

   Use

   Tags

   mCherry

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Available Since

   April 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAD-CMV-STAT5B-DN-CMV-GFP

   Plasmid

   #83262

   Purpose

   Adenoviral expression of dominant negative STAT5B with co-expression of GFP

   Depositor

   Andrew Brooks

   Article

   Legrand et al J Invest Dermatol. 2016 Sep;136(9):1781-

   Inserts

   Stat5b (Stat5b Mouse)
   eGFP
   

   Use

   Adenoviral

   Tags

   FLAG

   Expression

   Mutation

   dominant negative (Y699F)

   Promoter

   CMV

   Available Since

   July 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. All-in-one split ADAR2(E488Q)

   Plasmid

   #170137

   Purpose

   AAV vector carrying ADAR2-DDC(E488Q)-λN-P2A-MCP-ADAR2-DDN with a nuclear export signal

   Depositor

   Prashant Mali

   Article

   Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

   Insert

   ADAR2-DDC(E488Q)-λN-P2A-MCP-ADAR2-DDN
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA_DiCre_60.F2

   Plasmid

   #60208

   Purpose

   Expresses a fusion protein of FRB-linker peptide 2- Cre(60-343)

   Depositor

   Jean-Paul Herman

   Article

   Jullien et al Nucleic Acids Res. 2003 Nov 1;31(21):e13

   Insert

   Cre recombinase (CD207 Bacteriophage P1, Synthetic, Human)
   

   Use

   Tags

   FRAP and Nuclear localisation signal

   Expression

   Mammalian

   Mutation

   amino acids 2021-2112 of FRAP with a T2098L mutat…

   Promoter

   CMV

   Available Since

   Feb. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGG-TurboID-GFP-PTS1

   Plasmid

   #209406

   Purpose

   Transiently expressing and visualizing the subcellular localization of TurboID fused with a peroxisomal targeting signal type I (PTS1) in planta

   Depositor

   Rosa Lozano-Durán

   Article

   Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

   Insert

   3XHA-TurboID-PTS1
   

   Use

   Tags

   GFP

   Expression

   Plant

   Mutation

   TurboID gene sequence C249A

   Promoter

   Cauliflower mosaic virus (CaMV) 35S promoter

   Available Since

   Jan. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. ZIKV_Trigger_32B

   Plasmid

   #75009

   Purpose

   Portion of Zika Virus genome (KU312312: 7166-7299) containing sensor 32B trigger sequence

   Depositor

   Alexander Green, James Collins

   Article

   Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

   Insert

   ZIKV_Trigger_32B
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   March 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCS2-ER-EmEGFP

   Plasmid

   #169000

   Purpose

   Express ER-EmEGFP in zebrafish

   Depositor

   Noboru Mizushima

   Article

   Morishita et al Nature. 2021 Apr;592(7855):634-638. doi:

   Insert

   ER-EGFP (encoding the signal sequence of human calreticulin mRNA and EGFP-KDEL) (CALR Synthetic, Human)
   

   Use

   Unspecified

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Available Since

   June 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. MSP7-bio

   Plasmid

   #47735

   Purpose

   Expresses enzymatically monobiotinylated full-length MSP7 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

   Depositor

   Gavin Wright

   Article

   Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

   Insert

   Codon-optimised MSP7
   

   Use

   Tags

   enzymatic biotinylation sequence and rat Cd4 d3+4

   Expression

   Mammalian

   Mutation

   Exogenous signal peptide of mouse origin, changed…

   Promoter

   CMV

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. MSP5-bio

   Plasmid

   #47718

   Purpose

   Expresses enzymatically monobiotinylated full-length MSP5 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

   Depositor

   Gavin Wright

   Article

   Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

   Insert

   Codon-optimised MSP5
   

   Use

   Tags

   enzymatic biotinylation sequence and rat Cd4 d3+4

   Expression

   Mammalian

   Mutation

   Exogenous signal peptide of mouse origin, changed…

   Promoter

   CMV

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. RhopH3-bio

    Plasmid

    #47781

    Purpose

    Expresses enzymatically monobiotinylated full-length RhopH3 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised RhopH3
    

    Use

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Twitch-3 pRSETB

    Plasmid

    #48204

    Purpose

    Fluorescent reporter for calcium signaling

    Depositor

    Oliver Griesbeck

    Article

    Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

    Insert

    Twitch-3A
    

    Use

    Tags

    6xHIS, cpVenusCD, and mCerulean3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pUPD2 HDV (GB1444)

    Plasmid

    #160576

    Purpose

    Hepatitis Delta Virus ribozyme signal for the assembly of gRNA expression cassettes.

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

    Insert

    HDV
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    U6-26

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. 3xFN-Tel-TAL/pCMV-7.1

    Plasmid

    #63589

    Purpose

    Expresses a 3xFLAG-tagged TAL protein recognizing a telomere repeat.

    Depositor

    Hodaka Fujii

    Article

    Fujita et al Sci Rep. 2013 Nov 8;3:3171. doi: 10.1038

    Insert

    3xFVN-Tel-TAL
    

    Use

    TALEN

    Tags

    3xFLAG-tag, V5-tag, and nuclear localization sign…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Myc-gamma2s

    Plasmid

    #118036

    Purpose

    GABAA receptor expression (gamma2 short subunit) Myc-tag near N-terminus

    Depositor

    Angel de Blas

    Article

    Christie et al Mol Cell Neurosci. 2006 Jan;31(1):1-14.

    Insert

    GABRG2 (Gabrg2 Rat)
    

    Use

    Tags

    cMyc epitope EQKLISEEDL inserted between fourth a…

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pT2-7xTcf-NLS-ONL

    Plasmid

    #65713

    Purpose

    Tol2-based Wnt signal reporter plasmid (expresses NLS-ONL)

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    7xTcf, minCMV, 3xNLS, ONL
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRSET a sfGCaMP6s-T78H

    Plasmid

    #100023

    Purpose

    Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. cpEGFP replaced with a superfolder cpGFP variant containing T78H mutation

    Depositor

    Wolf Frommer

    Article

    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

    Insert

    sfGCaMP6s-T78H
    

    Use

    Tags

    6x HIS tag and Xpress_EK tag

    Expression

    Bacterial

    Mutation

    cpEGFP replaced with superfolder cpGFP and T78H m…

    Promoter

    T7

    Available Since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. C25-pLAC

    Plasmid

    #45283

    Purpose

    lacritin-intein fusion plasmid coding for truncated lacritin without signal peptide

    Depositor

    Gordon Laurie

    Article

    Wang et al J Cell Biol. 2006 Aug 28;174(5):689-700.

    Insert

    pLAC-C25 (LACRT Human)
    

    Use

    Protein expression vector

    Tags

    Intein

    Expression

    Bacterial

    Mutation

    contains aa20-113 of lacritin

    Promoter

    T7

    Available Since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFastBacDual with LFn + PrgJ

    Plasmid

    #84869

    Purpose

    Baculovirus Expression of LFn-PrgJ fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel protein

    Depositor

    Russell Vance

    Article

    Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

    Inserts

    LFn
    PrgJ
    

    Use

    Baculovirus

    Tags

    6xHis

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available Since

    Nov. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAD-CMV-STAT5A-DN-CMV-GFP

    Plasmid

    #83252

    Purpose

    Adenoviral expression of STAT5A dominant negative with co-expression of GFP

    Depositor

    Andrew Brooks

    Article

    Legrand et al J Invest Dermatol. 2016 Sep;136(9):1781-

    Inserts

    Stat5a (Stat5a Mouse)
    eGFP
    

    Use

    Adenoviral

    Tags

    Expression

    Mutation

    C-terminal deletion of 135 nucleotides (dominant …

    Promoter

    CMV

    Available Since

    Sept. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-hSyn-mCherry-T2A-miniSOG-C-Far

    Plasmid

    #193915

    Purpose

    Expresses miniSOG targeted to the plasma membrane of neurons, joined by a T2A cleavage site with mCherry; for AAV production

    Depositor

    Xiao Wang

    Article

    Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

    Insert

    miniSOG
    

    Use

    AAV

    Tags

    Farnesylation signal sequence and Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available Since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge