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pEasyG3_nat

Plasmid

#184918

Purpose

Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_nat recombines in vivo with a PCR product from pEasyG3_mic.

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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LbdCas12a KRAB

Plasmid

#188503

Purpose

Expresses FLAG tagged LbdCas12a KRAB

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12a

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D832A

Promoter

EF1a

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pEVOL-MmAcKRS1-PylTUUA

Plasmid

#182537

Purpose

N‐acetyl lysyl‐tRNA synthetase with cognate tRNA for genetic code expansion with ochre suppression

Depositor

Wenshe Liu

Article

Wu et al Chembiochem. 2012 Jul 9;13(10):1405-8. d

Insert

PylTUUA

Expression

Bacterial

Promoter

ProK

Available Since

April 28, 2022

Availability

Academic Institutions and Nonprofits only

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FASTHDR-Cterm-NanoLuc-Puromycin

Plasmid

#167209

Purpose

Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with NanoLuc and selection with Puromycin

Depositor

Oscar Perez

Article

Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

Type

Empty backbone

Use

CRISPR

Tags

NanoLuc

Expression

Mammalian

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pEntr1a-wGBD-mTurquoise2-wGBD

Plasmid

#176136

Purpose

Visualization of active Cdc42

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

WAS (WAS Human)

Use

Gateway Cloning

Available Since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1P POLD1_2

Plasmid

#160792

Purpose

Suppress POLD1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shPOLD1_2

Use

Lentiviral

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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MAC-SARS-CoV-2 NSP8

Plasmid

#158359

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 NSP8 (ORF1ab )

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-SARS-CoV-2 NSP10

Plasmid

#158361

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 NSP10 (ORF1ab )

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT-TO-eGFP-HF

Plasmid

#157735

Purpose

mammalian expression of C-terminally His8-Flag tagged eGFP under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

eGFP-HF

Tags

His8-Flag

Expression

Mammalian

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Nsp12

Plasmid

#157709

Purpose

mammalian expression of untagged SARS-CoV-2 Nsp12 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp12 (ORF1ab SARS-CoV-2)

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT-TO-Nsp9

Plasmid

#157703

Purpose

mammalian expression of untagged SARS-CoV-2 Nsp9 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp9 (ORF1ab SARS-CoV-2)

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT-TO-Nsp8

Plasmid

#157694

Purpose

mammalian expression of untagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp8 (ORF1ab SARS-CoV-2)

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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px330-UFL1 sgRNA2

Plasmid

#134636

Purpose

contains sgRNA targeting human UFL1 for gene knockout

Depositor

Yihong Ye

Article

Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

Insert

UFL1 sgRNA2 (UFL1 Human)

Expression

Mammalian

Available Since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pNAS1b split mCherry 14-3-3β

Plasmid

#111880

Purpose

Mode #2 phosphosite expression (split mCherry, using 14-3-3β binding domain)

Depositor

Jesse Rinehart

Article

Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

Type

Empty backbone

Tags

Split mCherry system: 14-3-3β fused to C-terminal…

Expression

Bacterial

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-REDD1 R133A/P139A (#626)

Plasmid

#99946

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

Insert

REDD1 (DDIT4 Human)

Tags

HA

Expression

Mammalian

Mutation

R133A/P139A

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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YIp128-P30-His-POL30

Plasmid

#99542

Purpose

Yeast integrative vector for expression of His-tagged POL30 (PCNA); LEU2 marker

Depositor

Helle Ulrich

Article

Davies et al Methods Mol Biol. 2012;920:543-67. doi:

Insert

POL30 (POL30 Budding Yeast)

Tags

6xHis

Expression

Yeast

Promoter

POL30

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pT2-cryR;dll4P1Egfp

Plasmid

#90160

Purpose

contains dll4 specific gene promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

dll4P1Egfp (dll4 Zebrafish)

Tags

EGFP

Expression

Bacterial

Available Since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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CAG-mCherry-Otx2shRNA1

Plasmid

#73979

Purpose

Otx2 shRNA1 (mir155 backbone) was inserted into the 3'UTR of mCherry.

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

shRNA that targets the mouse Otx2 gene

Expression

Mammalian

Promoter

CAG

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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pET23a_mod_RBPMS_FL

Plasmid

#59393

Purpose

Plasmid for bacterial overexpression of RBPMS

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Tags

6xHis, FLAG, and HA

Expression

Bacterial

Promoter

T7

Available Since

Feb. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pRS424-TUB2-Cterminal6xHis

Plasmid

#60386

Purpose

Expression of yeast beta tubulin (TUB2)-Cterminal 6xHis using a GAL promoter

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB2

Tags

6xHIS

Expression

Yeast

Promoter

GAL

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only