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pBN266

Plasmid

#86732

Purpose

Tissue-specific FLP expression plasmid for C. elegans

Depositor

Peter Askjaer

Article

Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

Insert

Pdpy-7::FLP D5

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCFJ66

Plasmid

#24981

Depositor

Erik Jorgensen

Article

Frokjær-Jensen et al Nat Methods. 2010 Apr 25. ():.

Insert

Cbr-unc-119 Punc-122::GFP

Use

Multisite gateway

Tags

Expression

Mutation

Promoter

Available since

Aug. 4, 2010

Availability

Academic Institutions and Nonprofits only

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pJH2827

Plasmid

#179542

Purpose

Pnmr-1::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans AVA/E/D premotor IN and others expression of miniSOG-SL2 RFP

Depositor

Mei Zhen

Article

Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

Insert

tomm20::miniSOG (tomm-20 Nematode)

Use

Tags

wCherry

Expression

Worm

Mutation

Promoter

Pnmr-1

Available since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS EFF-1::V5

Plasmid

#133584

Purpose

Expresses EFF-1 A full length protein in mammalian cells

Depositor

Benjamin Podbilewicz

Article

Avinoam et al Science. 2011 Apr 29;332(6029):589-92. d

Insert

EFF-1::V5

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pEG345

Plasmid

#40077

Depositor

Geraldine Seydoux

Article

Griffin et al Cell. 2011 Sep 16;146(6):955-68.

Insert

Mex-5 (mex-5 Nematode)

Use

Tags

Dendra2 PFAF, S-peptide, and TEV protease cleavag…

Expression

Worm

Mutation

Promoter

pie-1

Available since

Sept. 10, 2012

Availability

Academic Institutions and Nonprofits only
pMLS234

Plasmid

#73682

Purpose

SapTrap 3-site Destination vector for N-terminal GFP tagging with embedded Cbr-unc-119 selectable marker

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

GFP + Cbr-unc-119

Use

CRISPR and Cre/Lox

Tags

GFP

Expression

Worm

Mutation

Promoter

Available since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCG34

Plasmid

#26940

Depositor

Geraldine Seydoux

Article

Gallo et al Dev Biol. 2008 Nov 1. 323(1):76-87.

Insert

cgh-1 (cgh-1 Nematode)

Use

Tags

GFP

Expression

Worm

Mutation

Promoter

Available since

June 22, 2011

Availability

Academic Institutions and Nonprofits only

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pJW1820

Plasmid

#154321

Purpose

Multi-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

GFP^SEC (LoxP)^AID*::3xFLAG

Use

CRISPR and Cre/Lox

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pClneoEGFPC RSF-1

Plasmid

#66862

Purpose

Express GFP-fused C.elegans RSF-1 in mammalian cells

Depositor

Yutaka Hata

Article

Iwasa et al Exp Cell Res. 2013 Feb 1;319(3):1-11. do

Insert

RSF-1 (rsf-1 Nematode)

Use

Tags

GFP

Expression

Mammalian

Mutation

S18G, S229T, I403T and F563S mutations in RSF-1 c…

Promoter

CMV

Available since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only
pAP635-2

Plasmid

#99485

Purpose

Myc::TEV::3XFlag::Dendra2::3XFlag::TEV::Myc

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

Myc::TEV::3XFlag::Dendra2::3XFlag::TEV::Myc

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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TC393

Plasmid

#122625

Purpose

Pser-2::rtetR-QFAD::P2A::mKate::T2A::tetR-pie1

Depositor

Tian Chi

Article

Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

Insert

rtetR-QFAD::P2A::mKate::T2A::tetR-pie1

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

May 28, 2019

Availability

Academic Institutions and Nonprofits only

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pCFJ1360 - Intron GG3 - smu-1

Plasmid

#159806

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

Intron GG3 - smu-1

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pMDJ15 - pEXP[Peft-3 | mCardinal (NLS) | tbb-2 UTR]

Plasmid

#159801

Purpose

Expression construct with ubiquitous expression to test fluorescence level on microscope

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Peft-3 | mCardinal (NLS) | tbb-2 UTR]

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCFJ1358 - Intron GG1 - smu-1

Plasmid

#159804

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

Intron GG1 - smu-1

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBN282

Plasmid

#86727

Purpose

Tissue-specific FLP expression plasmid for C. elegans

Depositor

Peter Askjaer

Article

Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

Insert

Pelt-2::FLP D5

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCM3.11

Plasmid

#19605

Depositor

Geraldine Seydoux

Insert

pgl-1 ORF+3' in pDONR201 (pgl-1 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only

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pCM5.57

Plasmid

#19378

Depositor

Geraldine Seydoux

Article

Merritt et al Curr Biol. 2008 Sep 23. ():.

Insert

spe-11 3'utr in pDONRP2RP3

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

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pBY2946

Plasmid

#23223

Depositor

Ralf Baumeister

Article

Schaffer et al Nucleic Acids Res. 2010 Jan 4. ():.

Insert

SnAvi

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

May 4, 2010

Availability

Academic Institutions and Nonprofits only

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pCM1.153

Plasmid

#21387

Depositor

Geraldine Seydoux

Article

Merritt et al Curr Biol. 2008 Sep 23. ():.

Insert

gld-1 M1M2 3'utr in pDONRP2RP3

Use

Gateway

Tags

Expression

Mutation

Promoter

Available since

June 19, 2009

Availability

Academic Institutions and Nonprofits only

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pJL1604

Plasmid

#78194

Purpose

Ce cha-1 expression in motor neurons in worms

Depositor

Junho Lee

Article

Lee et al Nat Neurosci. 2011 Nov 13;15(1):107-12.

Insert

cha-1 (cha-1 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

unc-3

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only