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pAP997-1

Plasmid

#99500

Purpose

3XFlag::tagRFP::linker::TEV

Depositor

Geraldine Seydoux

Publication

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

3XFlag::tagRFP::linker::TEV

Expression

Bacterial

Available since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pAP546-2

Plasmid

#99476

Purpose

3XFlag::mNeptune::Myc

Depositor

Geraldine Seydoux

Publication

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

3XFlag::mNeptune::Myc

Expression

Bacterial

Available since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pBN258

Plasmid

#86721

Purpose

Tissue-specific FLP expression plasmid for C. elegans

Depositor

Peter Askjaer

Publication

Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

Insert

Punc-119::FLP D5

Expression

Worm

Available since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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pCZGY2844

Plasmid

#87760

Purpose

Punc-17b-PH::miniSOG, expression of membrane targeted PH-miniSOG in cholinergic motor neurons.

Depositor

Andrew Chisholm

Publication

Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

Insert

Punc-17b-PH-miniSOG

Tags

PH

Expression

Worm

Promoter

Punc-17b

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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6xHis-TEV-GEI-17(133-509)

Plasmid

#87056

Purpose

Expresses 6xHis-tagged gei-17 (isoform f), with a TEV protease cleavage site between the tag and the ORF

Depositor

Ronald Hay

Publication

Pelisch et al Nat Commun. 2014 Dec 5;5:5485. doi: 10.1

Insert

GEI-17 isfoomr f (aa 133-509) (gei-17 Nematode)

Tags

6xHis-TEV and GAGGGS linker

Expression

Bacterial

Promoter

T7

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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6xHis-TEV-SMO-1(GG)

Plasmid

#87054

Purpose

Expresses 6xHis-tagged smo-1(GG), with a TEV protease cleavage site between the tag and the ORF

Depositor

Ronald Hay

Publication

Pelisch et al Nat Commun. 2014 Dec 5;5:5485. doi: 10.1

Insert

smo-1 (smo-1 Nematode)

Tags

6xHis-TEV

Expression

Bacterial

Mutation

The last aa was removed to render processed SMO-1…

Promoter

T7

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pBN40

Plasmid

#86715

Purpose

C. elegans injection marker

Depositor

Peter Askjaer

Publication

Morales-Martinez et al J Cell Sci. 2015 Mar 15;128(6):1090-6. d

Insert

Plmn-1::gfp::his-58

Expression

Worm

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pBN263

Plasmid

#86729

Purpose

Tissue-specific FLP expression plasmid for C. elegans

Depositor

Peter Askjaer

Publication

Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

Insert

Pmyo-2::FLP D5

Expression

Worm

Available since

Feb. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pCFJ150-cul4

Plasmid

#80353

Purpose

Pmex-5:GFP::H2B::PEST::cul-4 3'UTR reporter construct used for microinjection

Depositor

Sean Ryder

Publication

Kaymak et al Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.

Insert

Pmex-5:GFP::H2B::PEST::cul-4 3'UTR

Expression

Worm

Available since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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TU#1450_unc-53a_cDNA

Plasmid

#82361

Purpose

express unc-53a cDNA

Depositor

Martin Chalfie

Publication

Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

unc-53 a isoform (unc-53 Nematode)

Expression

Worm

Promoter

none

Available since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pJL1703

Plasmid

#78196

Purpose

optogenetic activation of IL2 neuron in worms

Depositor

Junho Lee

Publication

Lee et al Nat Neurosci. 2011 Nov 13;15(1):107-12.

Insert

ChR2(gf)

Expression

Worm

Promoter

klp-6

Available since

July 29, 2016

Availability

Academic Institutions and Nonprofits only
pJJR50

Plasmid

#75026

Purpose

U6 promoter driven flipped + extended sgRNA expression vector

Depositor

Mike Boxem

Publication

Waaijers et al BMC Biol. 2016 Aug 9;14:66. doi: 10.1186

Insert

guide RNA, flipped and extended version

Use

CRISPR

Promoter

R07E5.16 (U6)

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pMLS285

Plasmid

#73734

Purpose

SapTrap egl-13 NLS connector donor plasmid

Depositor

Erik Jorgensen

Publication

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

egl-13 NLS

Use

CRISPR

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pMLS269

Plasmid

#73727

Purpose

SapTrap SL2::SV40-NLS C-terminal connector donor plasmid

Depositor

Erik Jorgensen

Publication

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

SL2 + SV40 NLS (gdp-2/gdp-3 intergenic region)

Use

CRISPR

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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JH05

Plasmid

#73516

Purpose

mYPet_PH pCFJ150 - contain C. elegans codon optimized mYPet

Depositor

Bob Goldstein

Publication

Heppert et al Mol Biol Cell. 2016 Nov 7;27(22):3385-33

Insert

pmex-5::mPYet::PH::tbb-2

Tags

mYPet

Promoter

mex-5

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pKPY738

Plasmid

#72674

Purpose

C. elegans let-858 3' UTR (Gateway Entry Clone)

Depositor

David Tirrell

Insert

let-858 3' UTR [From pPD129.57 (+2554 to +2960)] in pDONR P2R-P3

Use

Gateway Cloning

Available since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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FLAG-HA-BLMP-1-pcDNA3.1-

Plasmid

#52512

Purpose

expresses C. elegans BLMP-1 in mammalian cells with FLAG-HA tag at N-terminus

Depositor

Adam Antebi

Publication

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Insert

Blmp-1 (blmp-1 Nematode)

Tags

FLAG-HA

Expression

Mammalian

Mutation

N75S mutation compared GenBank reference sequence…

Promoter

CMV

Available since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pSR29

Plasmid

#69255

Purpose

pelt-2::CRE for integration on ttTi14024, Chr X

Depositor

Sander van den Heuvel

Publication

Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

Insert

Cre recombinase

Use

Cre/Lox; Mossci

Expression

Worm

Mutation

codon-optimzed index 1.0, tbb-2 UTR is in wrong o…

Promoter

elt-2

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pClneoFH-let60 G12V

Plasmid

#66860

Purpose

Express FLAG-His6-tagged dominant active let60, in which Glycine 12 is mutated to Valine, in mammalian cells

Depositor

Yutaka Hata

Publication

Iwasa et al Exp Cell Res. 2013 Feb 1;319(3):1-11. do

Insert

Let-60 (let-60 Nematode)

Tags

FLAG-His6

Expression

Mammalian

Mutation

Glycine 12 is mutated to valine

Promoter

CMV

Available since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
FLAG-HA-DRE-1-pcDNA3.1-

Plasmid

#52513

Purpose

expresses C. elegans DRE-1 in mammalian cells with FLAG-HA tag at N-terminus

Depositor

Adam Antebi

Publication

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Insert

Dre-1 (dre-1 Nematode)

Tags

FLAG-HA

Expression

Mammalian

Promoter

CMV

Available since

July 31, 2015

Availability

Academic Institutions and Nonprofits only

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