Skip to main content
Addgene
Showing: 1051 - 1080 of 6393 results

  1. pVSL10

    Plasmid
    #201182
    Purpose
    Bst polymerase large fragment (BF) F710Y/D598A, His6-tagged, HRV 3C-cleavable
    Depositor
    Jack Szostak
    Article
    Lelyveld et al Science. 2023 Oct 27;382(6669):423-429.
    Insert
    Bst polymerase large fragment (BF) F710Y/D598A
    Use
    Tags
    HRV 3C protease cleavage site and His6
    Expression
    Bacterial
    Mutation
    F710Y D598A
    Promoter
    T5-lacO
    Available since
    Sept. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    201182-plasmid-map-sequence-id-406202

    Enlarge

  6. CDS12-MpER-Targ

    Plasmid
    #136094
    Purpose
    CDS12 with ER targeting peptide to fuse with CTAG containing HDEL ER retention signal at the 3'end
    Depositor
    Jim Haseloff
    Article
    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102
    Insert
    ER targeting peptide from predicted chitinase Mapoly0069s0092
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    BsaI/ SapI domesticated
    Promoter
    Available since
    Nov. 30, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    136094-plasmid-map-sequence-id-275025

    Enlarge

  7. pCRISPRyl

    Plasmid
    #70007
    Purpose
    CRISPR/Cas9 vector for Yarrowia lipolytica, with AvrII site for sgRNA insertion
    Depositor
    Ian Wheeldon
    Article
    Schwartz et al ACS Synth Biol. 2015 Dec 29.
    Inserts
    Codon optimized Cas9 from S. pyogenes
    sgRNA expression cassette
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    SCR1'-tRNA and UAS1B8-TEF(136)
    Available since
    Feb. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    70007-plasmid-map-sequence-id-137578

    Enlarge

  11. pCMV6-AN-HA_aa 1-542 POLK

    Plasmid
    #201585
    Purpose
    Expresses the N-terminal clasp domain and catalytic domain (aa 1-542) of human DNA polymerase kappa with a HA tag in mammalian cells
    Depositor
    Roger Woodgate
    Article
    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1
    Insert
    Polymerase kappa (POLK Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Expresses amino acids 1-542 of DNA polymerase kap…
    Promoter
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    201585-plasmid-map-sequence-id-395030

    Enlarge

  12. pCCM

    Plasmid
    #162708
    Purpose
    Second CCM operon cloned from H. neapolitanus; pFA expressing the remaining 11 genes in the H. neapolitanus CCM cluster
    Depositor
    David Savage
    Article
    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1
    Insert
    Second CCM operon cloned from H. neapolitanus
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLtet0-1 promoter
    Available since
    Feb. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    162708-plasmid-map-sequence-id-324195

    Enlarge

  13. Linked VLP NES

    Plasmid
    #182432
    Purpose
    Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-ERG20-AcNES1 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al bioRxiv 2022.11.24.517869
    Inserts
    VP2C-FPPS-NES
    deltaVP1
    Use
    Synthetic Biology; Metabolic engineering
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1 and GAL10
    Available since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182432-plasmid-map-sequence-id-360114

    Enlarge

  14. Linked VLP NES (M)

    Plasmid
    #182434
    Purpose
    YIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-ERG20(F96W-N127W)-AcNES1 (GAL10 promoter). Contains uracil marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al bioRxiv 2022.11.24.517869
    Inserts
    VP2C-FPPS(M)-NES
    deltaVP1
    Use
    Synthetic Biology; Metabolic engineering
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1 and GAL10
    Available since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182434-plasmid-map-sequence-id-360117

    Enlarge

  15. Linked VLP NES (G)

    Plasmid
    #182476
    Purpose
    YIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-ERG20(F96C)-AcNES1 (GAL10 promoter). Contains uracil marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al bioRxiv 2022.11.24.517869
    Inserts
    VP2C-FPPS(G)-NES
    deltaVP1
    Use
    Synthetic Biology; Metabolic engineering
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1 and GAL10
    Available since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182476-plasmid-map-sequence-id-360123

    Enlarge

  16. deltaVP1 + VP2C-GFP

    Plasmid
    #166676
    Purpose
    Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and GFP tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3)
    Depositor
    Claudia Vickers
    Article
    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-
    Inserts
    VP2C-GFP
    Murine polyomavirus deltaVP1 (NLS deletion mutant)
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1 and GAL10
    Available since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    166676-plasmid-map-sequence-id-329830

    Enlarge

  18. Coexpressed VLP NES

    Plasmid
    #182433
    Purpose
    Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-ERG20 (GAL10 promoter), and VP2C-AcNES1 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3)
    Depositor
    Claudia Vickers
    Article
    Cheah et al bioRxiv 2022.11.24.517869
    Inserts
    VP2C-FPPS
    deltaVP1
    VP2C-NES
    Use
    Synthetic Biology; Metabolic engineering
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1, GAL10, and GAL7
    Available since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182433-plasmid-map-sequence-id-360116

    Enlarge

  19. Coexpressed VLP NES (M)

    Plasmid
    #182435
    Purpose
    YIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-ERG20(F96W-N127W) (GAL10 promoter), and VP2C-AcNES1 (GAL7 promoter). Contains uracil marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al bioRxiv 2022.11.24.517869
    Inserts
    VP2C-FPPS(M)
    deltaVP1
    VP2C-NES
    Use
    Synthetic Biology; Metabolic engineering
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1, GAL10, and GAL7
    Available since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182435-plasmid-map-sequence-id-360119

    Enlarge

  20. Coexpressed VLP NES (G)

    Plasmid
    #182477
    Purpose
    YIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-ERG20(F96C) (GAL10 promoter), and VP2C-AcNES1 (GAL7 promoter). Contains uracil marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al bioRxiv 2022.11.24.517869
    Inserts
    VP2C-FPPS(G)
    deltaVP1
    VP2C-NES
    Use
    Synthetic Biology; Metabolic engineering
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1, GAL10, and GAL7
    Available since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182477-plasmid-map-sequence-id-360429

    Enlarge

  21. wtVP1 + VP2C-GFP

    Plasmid
    #166674
    Purpose
    Yeast integrative plasmid for expressing wild-type Murine polyomavirus VP1 (GAL1 promoter) and GFP tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-
    Inserts
    VP2C-GFP
    Wild-type Murine polyomavirus VP1
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1 and GAL10
    Available since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    166674-plasmid-map-sequence-id-329826

    Enlarge

  22. pCOA-Leu-FvPPT1-fsr1

    Plasmid
    #179868
    Purpose
    Constitutive expression vector for Saccharomyces cerevisiae comprising the polyketide synthase fsr1 from Fusarium vanettenii and the Fusarium verticillioides 4´-phosphopantetheinyltransferase PPT1
    Depositor
    Jens Laurids Sørensen
    Article
    Bejenari et al J Microbiol Biol Educ. 2023 Nov 27;24(3)
    Inserts
    PPT1
    fsr1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Codon optimized for expression in Saccharomyces c…
    Promoter
    Yarrowia lipolytica EXP1 and Yarrowia lipolytica …
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    179868-plasmid-map-sequence-id-352936

    Enlarge

  23. pCfb12567

    Plasmid
    #219927
    Purpose
    PrCYC with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI
    Depositor
    Irina Borodina
    Insert
    Promoter CYC
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    219927-plasmid-map-sequence-id-460324

    Enlarge

  24. pCfb12566

    Plasmid
    #219926
    Purpose
    PrDGA with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI
    Depositor
    Irina Borodina
    Insert
    Promoter DGA
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    219926-plasmid-map-sequence-id-460318

    Enlarge

  25. pCfb12622

    Plasmid
    #219929
    Purpose
    PrACT with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI
    Depositor
    Irina Borodina
    Insert
    Promoter ACT
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    219929-plasmid-map-sequence-id-436186

    Enlarge

  26. pCfb12562

    Plasmid
    #219923
    Purpose
    PrGPD with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI
    Depositor
    Irina Borodina
    Insert
    Promoter GPD
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    219923-plasmid-map-sequence-id-460320

    Enlarge

  27. pCfb13186

    Plasmid
    #219924
    Purpose
    PrTEF with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI
    Depositor
    Irina Borodina
    Insert
    Promoter TEF
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    219924-plasmid-map-sequence-id-436038

    Enlarge

  28. pCfb12620

    Plasmid
    #219928
    Purpose
    PrTPI with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI
    Depositor
    Irina Borodina
    Insert
    Promoter TPI
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    219928-plasmid-map-sequence-id-435947

    Enlarge

  29. pCfb12561

    Plasmid
    #219922
    Purpose
    PrPGM with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI
    Depositor
    Irina Borodina
    Insert
    Promoter PGM
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    219922-plasmid-map-sequence-id-435639

    Enlarge

  30. pCfb12565

    Plasmid
    #219925
    Purpose
    PrFBA with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI
    Depositor
    Irina Borodina
    Insert
    Promoter FBA
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    219925-plasmid-map-sequence-id-435638

    Enlarge

Showing: 1051 - 1080 of 6393 results