We narrowed to 1,445 results for: pBAD
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Plasmid#124292PurposeExpression of vsfGFP-9. This construct improves brightness of sfGFP by fusing a GFP-specific single domain antibody to it that creates a monomeric fluorophore.DepositorInsertvsfGFP-9
ExpressionBacterialAvailable SinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBRC901
Plasmid#167948Purposeexpress wild-type bacterial tyrosine kinase Wzc from E. coli E69 (O9a:K30)DepositorInsertwild-type bacterial tyrosine kinase Wzc from E. coli E69 (O9a:K30)
Tagshexa-histidine tagExpressionBacterialPromoteraraBADAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSLC-254
Plasmid#124291PurposeExpression of vsfGFP-0. This construct improves brightness of sfGFP by fusing a GFP-specific single domain antibody to it that creates a dimeric fluorophore.DepositorInsertvsfGFP-0
ExpressionBacterialAvailable SinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPgiCas13b
Plasmid#184833PurposeInducible expression of PgiCas13b nuclease in bacteria.DepositorInsertCas13b nuclease from Porphyromonas gingivalis
UseCRISPRExpressionBacterialPromoterT7 promoterAvailable SinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFnCpf1GG
Plasmid#131010PurposeBackbone plasmid for generating CRISPR arrays for FnCas12a. Contains a GFP-dropout cassette and a direct repeat.DepositorInsertsdirect repeat of FnCas12a
GFP expression cassette
UseCRISPRExpressionBacterialAvailable SinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
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C-BLa 2.0
Plasmid#92060PurposeThe C-BLa 2.0 plasmid encodes a protein, where the TMD is connected to the C-terminus of the cytoplasmic domain of the ToxR protein and to the N-terminus of the C-terminal beta-lactamase fragment.DepositorInsertC-BLa 2.0 fusion protein
TagsFlag (DYKDHDG)ExpressionBacterialPromoteraraBADAvailable SinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLsCas13aGG
Plasmid#131012PurposeBackbone plasmid for generating CRISPR arrays for LsCas13a. Contains a GFP-dropout cassette and a direct repeat.DepositorInsertsdirect repeat of LsCas13a
GFP expression cassette
UseCRISPRExpressionBacterialAvailable SinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAsCpf1GG
Plasmid#131011PurposeBackbone plasmid for generating CRISPR arrays for AsCas12a. Contains a GFP-dropout cassette and a direct repeat.DepositorInsertsdirect repeat of AsCas12a
GFP expression cassette
UseCRISPRExpressionBacterialAvailable SinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBN201
Plasmid#217917Purposeexpress E. coli E69 (O9a:K30) Wzc_K540M with Y715E, Y717E, Y718E mutationsDepositorInsertE. coli E69 (O9a:K30) Wzc_K540M with Y715E, Y717E, Y718E mutations
Tagshexa-histidine tagExpressionBacterialMutationK540M, Y715E, Y717E, Y718EAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBN363
Plasmid#217918Purposeexpress E. coli E69 (O9a:K30) Wzc_K540M with Y715E, Y717E, Y718E and N711Y mutationsDepositorInsertE. coli E69 (O9a:K30) Wzc_K540M with Y715E, Y717E, Y718E and N711Y mutations
Tagshexa-histidine tagExpressionBacterialMutationK540M, Y715E, Y717E, Y718E, N711YAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBN413
Plasmid#217919Purposeexpress E. coli E69 (O9a:K30) Wzc with Y715E, Y717E, Y718E and N711Y mutationsDepositorInsertE. coli E69 (O9a:K30) Wzc with Y715E, Y717E, Y718E and N711Y mutations
Tagshexa-histidine tagExpressionBacterialMutationY715E, Y717E, Y718E, N711YAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pWzyE-His
Plasmid#196669PurposeExpression of C-terminally His10-tagged WzyE from Pectobacterium atrosepticum in E.coliDepositorInsertWzyE
TagsTEV cleavable His10ExpressionBacterialPromoteraraBADAvailable SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBN216
Plasmid#167963Purposeexpress E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E, Y718EDepositorInsertE. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E, Y718E
Tagshexa-histidine tagExpressionBacterialMutationY713E, Y715E, Y717E, Y718EPromoteraraBADAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBN217
Plasmid#167964Purposeexpress E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E, Y718EDepositorInsertE. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E, Y718E
Tagshexa-histidine tagExpressionBacterialMutationY708E, Y713E, Y715E, Y717E, Y718EPromoteraraBADAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBN262
Plasmid#167967Purposeexpress E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717EDepositorInsertE. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E
Tagshexa-histidine tagExpressionBacterialMutationY708E, Y713E, Y715E, Y717EPromoteraraBADAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBN202
Plasmid#167950Purposeexpress E. coli E69 (O9a:K30) Wzc_K540M with Y713E, Y715E, Y717E, Y718E mutationsDepositorInsertE. coli E69 (O9a:K30) Wzc_K540M with Y713E, Y715E, Y717E, Y718E mutations
Tagshexa-histidine tagExpressionBacterialMutationK540M, Y713E, Y715E, Y717E, Y718EPromoteraraBADAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
C-BLa 1.2
Plasmid#89000PurposeBLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.2 fusionprotein containing the C-terminal fragment of the split β-lactamase.DepositorInsertC-BLa 1.2 fusion protein
TagsFlagExpressionBacterialPromoteraraBADAvailable SinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
C-BLa 1.1
Plasmid#88997PurposeBLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.1 fusionprotein containing the C-terminal fragment of the split β-lactamase.DepositorInsertC-BLa 1.1 fusion protein
TagsFlagExpressionBacterialPromoteraraBADAvailable SinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCas9GG
Plasmid#131009PurposeBackbone plasmid for generating CRISPR arrays for SpCas9 using CRATES. Contains a direct repeat and a RFP-dropout cassette.DepositorInsertsdirect repeat of SpCas9
promoter PJ23119
mRFP expression cassette
UseCRISPRExpressionBacterialPromoterBba_R0040 TetRAvailable SinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPglL-strep-mClover3
Plasmid#196665PurposeExpression of C-terminally distrep tag II-mClover3-tagged PglL from Neisseria meningitidis in E.coliDepositorInsertPglL
TagsTEV-cleavable di-strep tag II and mClover3ExpressionBacterialPromoteraraBADAvailable SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWzyE-strep-mClover3
Plasmid#196668PurposeExpression of C-terminally distrep tag II-mClover3-tagged WzyE from Pectobacterium atrosepticum in E.coliDepositorInsertWzyE
TagsTEV-cleavable di-strep tag II and mClover3ExpressionBacterialPromoteraraBADAvailable SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCas9FnCpf1GG
Plasmid#131013PurposeBackbone plasmid for generating CRISPR arrays for composite array utilized by SpCas9 and FnCas12a using CRATES. Contains a GFP-dropout cassette and two direct repeats.DepositorInsertsdirect repeat of SpCas9
GFP expression cassette
direct repeat of FnCas12a
UseCRISPRExpressionBacterialAvailable SinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRD395
Plasmid#163102PurposeExpression of mCherry_H-NSdbd in Escherichia coli (and, potentially, other bacteria)DepositorInsertmCherry_H-NSdbd
ExpressionBacterialPromoteraraBAD promoterAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRD404
Plasmid#163109PurposeExpression of mNeonGreen_H-NSdbd in Escherichia coli (and, potentially, other bacteria)DepositorInsertmNeonGreen_H-NSdbd
ExpressionBacterialPromoteraraBAD promoterAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRD403
Plasmid#163108PurposeExpression of mScarlet-I_H-NSdbd in Escherichia coli (and, potentially, other bacteria)DepositorInsertmScarlet-I_H-NSdbd
ExpressionBacterialPromoteraraBAD promoterAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRD128
Plasmid#163097PurposeExpression of mTurquoise2_H-NSdbd in Escherichia coli (and, potentially, other bacteria)DepositorInsertmTurquoise2_H-NSdbd
ExpressionBacterialPromoteraraBAD promoterAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRD129
Plasmid#163098PurposeExpression of mEos3.2_H-NSdbd in Escherichia coli (and, potentially, other bacteria)DepositorInsertmEos3.2_H-NSdbd
ExpressionBacterialPromoteraraBAD promoterAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRD198
Plasmid#163101PurposeExpression of mEGFP_H-NSdbd in Escherichia coli (and, potentially, other bacteria)DepositorInsertmEGFP_H-NSdbd
ExpressionBacterialPromoteraraBAD promoterAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRD396
Plasmid#163103PurposeExpression of eYFP_H-NSdbd in Escherichia coli (and, potentially, other bacteria)DepositorInserteYFP_H-NSdbd
ExpressionBacterialPromoteraraBAD promoterAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
MB 101A ttrSR(m13)-sfGFP_mCherry
Plasmid#232474PurposeOptimized tetrathionate sensor with fluorescent reporter (GFP), constitutive mCherryDepositorInsertsttrS
ttrR
PttrB185-269
sfGFP
AxeTxe
mCherry
ExpressionBacterialPromoterConstitutive (TTGATAGCTAGCTCAGTCCTAGGTATTGTGCTAGC…Available SinceSept. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCas-dCas12m
Plasmid#192275PurposeEncodes MmdCas12m (D485A) under a constitutive promoterDepositorInsertMmdCas12m
UseCRISPRExpressionBacterialMutationD485APromoterPJ23108Available SinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCas-Cas12m
Plasmid#192274PurposeEncodes MmCas12m under a constitutive promoterDepositorInsertMmCas12
UseCRISPRExpressionBacterialPromoterPJ23108Available SinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTT021
Plasmid#226782PurposeExpression of wzxE and other genes from the E. coli enterobacterial common antigen biosynthesis gene clusterDepositorInsertwzxE, wecF, wzyE, wecG
ExpressionBacterialPromoterBADAvailable SinceJan. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCas-Cas12m-ΔZF
Plasmid#192276PurposeEncodes MmCas12m ΔZF (H549A, C552A) under a constitutive promoterDepositorInsertCas12m ΔZF
UseCRISPRExpressionBacterialMutationH459A, C552APromoterPJ23108Available SinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCas-dCas12m-ΔZF
Plasmid#192277PurposepCas-dCas12m-ΔZF (D485A, H549A, C552A) under a constitutive promoterDepositorInsertMmdCas12m ΔZF
UseCRISPRExpressionBacterialMutationD485A, H549A, C552APromoterPJ23108Available SinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-RFP entry
Plasmid#192278PurposeEntry vector for cloning MmCas12m spacers. RFP flanked by MmCas12m CRISPR repeats can be digested out with BbsI.DepositorInsertRFP flanked by type V-M CRISPR repeat sequences
UseCRISPRExpressionBacterialPromoterPJ23119Available SinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCas-MmdCas12m-CBE1
Plasmid#192284PurposeMmdCas12m-CBE1 expressed under a constitutive promoterDepositorInsertMmdCas12m-CDA-UGI
UseCRISPRExpressionBacterialMutationD485APromoterPJ23108Available SinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
MB 45t3 thsS(t3)R-sfGFP_mCherry
Plasmid#232470PurposeOptimized thiosulfate sensor with fluorescent reporter (GFP), constitutive mCherryDepositorInsertsthsS(t3)
thsR
PphsA342
sfGFP
AxeTxe
mCherry
ExpressionBacterialMutationContains the following mutations K286Q, Q350H, I…PromoterJ23100 (TTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGC), J23…Available SinceSept. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
MB 103C ttrSR(m13)-Bxb1_P7-sfGFP_mCherry
Plasmid#232475PurposeOptimized tetrathionate sensor with recombinase switch and fluorescent reporter (GFP), constitutive mCherryDepositorInsertsttrS
ttrR
PttrB185-269
sfGFP
AxeTxe
mCherry
Bxb1 integrase
TagsssrA degradation tagExpressionBacterialPromoterConstitutive (TTGATAGCTAGCTCAGTCCTAGGTATTGTGCTAGC…Available SinceNov. 20, 2025AvailabilityAcademic Institutions and Nonprofits only