We narrowed to 1,204 results for: GR

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MTK234_027

Plasmid

#123907

Purpose

Encodes the spCas9 sgRNA1 hThal5.10-2_hg18 (site 1) gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

sgRNA1 -hThal5.10-2_hg18

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK0_001

Plasmid

#123924

Purpose

Encodes the spCas9 gRNA GFP dropout expression cassette with ConLS and ConRE connectors with Ampicillin resistance as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

TU-sgRNA - L1/RE

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK0_049

Plasmid

#123946

Purpose

Encodes the hThal5.10-2_hg18 landing pad destination vector as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

hThal5.10-2_hg18 landing pad destination vector

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pYO286

Plasmid

#235730

Purpose

Protein expression of yeast VPS15 (1-981)-ZZ in yeast

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

VPS15

Tags

3xTEV-ZZ

Expression

Yeast

Mutation

aa 1-981

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pYO816

Plasmid

#242482

Purpose

Expression of Scatg38 Δ(123 to 226)-2xEGFP under its own promoter

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

ATG38 (YLR211C Budding Yeast)

Tags

2xEGFP

Expression

Yeast

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pYO1089

Plasmid

#242497

Purpose

Bacterial expression of GST-ScAtg38 Δ(123 to 226)

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

ATG38 (YLR211C Budding Yeast)

Tags

GST

Expression

Bacterial

Mutation

deletion of aa 123 to 226

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pOP768

Plasmid

#242496

Purpose

Bacterial expression of GST-ScAtg38 (87-226)

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

ATG38 (YLR211C Budding Yeast)

Tags

GST

Expression

Bacterial

Mutation

aa 87-226 only

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pYO780

Plasmid

#242481

Purpose

Expression of ScATG38 (FL)-2xEGFP under its own promoter

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

ATG38 (YLR211C Budding Yeast)

Tags

2xEGFP

Expression

Yeast

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pYO1091

Plasmid

#242499

Purpose

Bacterial expression of GST-ScAtg38 Δ(123 to 181)

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

ATG38 (YLR211C Budding Yeast)

Tags

GST

Expression

Bacterial

Mutation

deletion of aa 123 to 181

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pYO785

Plasmid

#242488

Purpose

Expression of ScAtg38 (FL)-3xFLAG under its own promoter

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

ATG38 (YLR211C Budding Yeast)

Tags

3xFLAG

Expression

Yeast

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pYO1093

Plasmid

#242501

Purpose

Bacterial expression of GST-ScAtg38 Δ(123 to 209)

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

ATG38 (YLR211C Budding Yeast)

Tags

GST

Expression

Bacterial

Mutation

deletion of aa 123 to 209

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pOP775

Plasmid

#242495

Purpose

Bacterial expression of GST-ScAtg38 (1-78)

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

ATG38 (YLR211C Budding Yeast)

Tags

GST

Expression

Bacterial

Mutation

aa 1-78 only

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pYO1092

Plasmid

#242500

Purpose

Bacterial expression of GST-ScAtg38 Δ(182 to 209)

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

ATG38 (YLR211C Budding Yeast)

Tags

GST

Expression

Bacterial

Mutation

deletion of aa 182 to 209

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pYO1090

Plasmid

#242498

Purpose

Bacterial expression of GST-ScAtg38 Δ(182 to 226)

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

ATG38 (YLR211C Budding Yeast)

Tags

GST

Expression

Bacterial

Mutation

deletion of aa 182 to 226

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pYO1094

Plasmid

#242502

Purpose

Bacterial expression of GST-ScAtg38 Δ(210 to 226)

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

ATG38 (YLR211C Budding Yeast)

Tags

GST

Expression

Bacterial

Mutation

deletion of aa 210 to 226

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pYO359

Plasmid

#235750

Purpose

Protein expression of FL ScVPS34 and FL ScVPS15-ZZ

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Inserts

VPS34 (VPS34 Budding Yeast)
VPS15 (VPS15 Budding Yeast)

Tags

3xTEV-ZZ

Expression

Yeast

Mutation

S2A, aa 3-887 and T134A, I851R

Promoter

GAL-TDH3

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgDHFR

Plasmid

#233898

Purpose

lentiviral vector expressing Cas9 and an sgRNA targeting DHFR

Depositor

Jason Cantor

Article

Flickinger et al Nat Metab. 2025 May 2. doi: 10.1038/s422

Insert

sgRNA targeting DHFR (DHFR Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-hsN-drAgo2

Plasmid

#221717

Purpose

Expression of zebrafish Ago2 with N-terminal 3xFLAG tag in zebrafish embryos. Swapped N-domain for human Ago2 N-domain.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

hsN-drAgo2 (ago2 Zebrafish)

Use

Fish expression

Tags

3xFLAG

Mutation

Swap of N-domain for human Ago2 N-domain

Promoter

simian CMV IE94

Available Since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-hsAgo2EF-DY

Plasmid

#221723

Purpose

Expression of human Ago2 with E637D and F666Y mutations and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

hsAgo2EF-DY (AGO2 Human)

Use

Fish expression

Tags

3xFLAG

Mutation

E637D and F666Y in active site

Promoter

simian CMV IE94

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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PGK Promoter (MTK 2) (pAN2822)

Plasmid

#194224

Purpose

Mammalian Toolkit part 2 encoding the PGK promoter

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv 2021.04.20.440678

Insert

PGK Promoter

Use

Synthetic Biology

Promoter

PGK

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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