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Plasmid#236039PurposeThe plasmid pQdCas12a.luxR(mut)-sggfp(A) expresses the dCas12a endonuclease and the sgRNA (design A) targeting the gfp gene. Additionally, it contains a mutation in the luxR gene.DepositorInsertquorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (B) of gfp gene
UseCRISPRTagsExpressionMutationPromoterquorum sensing promoterAvailable sinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
INT_GFPsp_CamRcargo
Plasmid#212143PurposePreparatory plasmid for gene disruption into the genome using CRISPR-Cas12a. Plasmid can be removed by incubating cells at 37 C.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, VchTnsA, VchTnsB, VchTnsC, green fluorescent protein
UseTagsExpressionBacterialMutationPromoterpJ23119Available sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKS_3MYC_PAC
Plasmid#122566PurposeVector for endogenously tagging Giardia genes with a triple Myc tag (Puromycin selection)DepositorTypeEmpty backboneUseTags3x c-Myc tagExpressionMutationPromoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKS_3HA_NEO
Plasmid#122565PurposeVector for endogenously tagging Giardia genes with a triple HA tag (G418 selection)DepositorTypeEmpty backboneUseTagshemagglutinin tagExpressionMutationPromoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL2315
Plasmid#137055PurposeloxP (empty) expression cassette: c-terminal GFPDepositorTypeEmpty backboneUseLeishmania floxed gene expressionTagsGFPExpressionMutationPromoterAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZS1katGp-RBS33-TP901-proD-oxyR
Plasmid#78219Purposesynthetic gene circuitDepositorInsertsynthetic gene circuit
UseTagsExpressionMutationPromoterAvailable sinceMay 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
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p350_LTJ_180bpCD90.1Template
Plasmid#104988Purpose180bp HDR template for murine CD90.1DepositorInsert180bp genomic DNA sequence from murine CD90.1
UseHdr template for gene editingTagsExpressionMutationPromoterAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSCrhaB2
Plasmid#113634PurposeAn expression vector containing a rhamnose-inducible promoter to regulate gene expression in Burkholderia cenocepaciaDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterPrhaBAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCVD364
Plasmid#63739PurposeV cholerae 395 nanH gene cloned into the BamHI site of pCVD315DepositorInsertnanH
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMo168
Plasmid#27389DepositorInsertpMo168
UseTagsExpressionBacterialMutationA replicative vector for gene expression in Burkh…PromoterAvailable sinceMarch 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
HisMS2_VLP_RSV
Plasmid#175958PurposeGeneration of MS2 Virus-Like Particles packaged with the sequence for the RSV P and M genes [GenBank: M74568.1]DepositorInsertsP gene
M gene
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMLS7
Plasmid#32056PurposeVector optimized for constitutive gene expression in Burkholderia genus and related bacteriaDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterPs7 promoter, originated from Paraburkholderia xe…Available sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBAD33.lon
Plasmid#22145DepositorInsertLon (lon E. coli K12)
UseTagsExpressionBacterialMutationNone, WT genePromoterAvailable sinceSept. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGPI-SceI
Plasmid#32060PurposeConstruction of unmarked gene deletions. Suicide cloning vector designed to introduce a targeted I-SceI restriction site in the genome of BurkholderiaDepositorTypeEmpty backboneUseMutagenesis plasmidTagsExpressionMutationPromoterAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
PRExpress
Plasmid#122486PurposeA non-leaky heat-inducible gene expression system for Drosophila. (GenBank: MF058598.1)DepositorInsertsLacZ
poly-PRE
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXPG
Plasmid#71248Purposeimproved luciferase reporter gene plasmidDepositorTypeEmpty backboneUseLuciferaseTagsluciferaseExpressionMammalianMutationPromoternoneAvailable sinceNov. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-LEU2MX6
Plasmid#33138Purposegene deletion in S. cerevisiae and S. pombeDepositorInsertLEU2MX6
UseTagsExpressionMutationPromoterAvailable sinceJan. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSD5
Plasmid#197289PurposeContains a seamless deletion cassette for the replacement of ORF2230 in D. radioduransDepositorInsertNptII
UseSynthetic Biology; Bacterial and yeast cloningTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQint
Plasmid#25819DepositorInsertqQint
UseBacterial gene inactivationTagsExpressionMutationplasmid to make targeted insertions in Clostridiu…PromoterAvailable sinceSept. 14, 2010AvailabilityAcademic Institutions and Nonprofits only