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pET28-sfGFP-DogTag Loop B

Plasmid

#171780

Purpose

Expresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Asp102 and Asp103

Depositor

Mark Howarth

Article

Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

Insert

sfGFP-DogTag Loop B

Use

Tags

His6

Expression

Bacterial

Mutation

DogTag and linkers inserted between residues Asp1…

Promoter

T7

Available since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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pET28-sfGFP-SpyTag003 Loop B

Plasmid

#171777

Purpose

Expresses in bacterial cytoplasm superfolder GFP with SpyTag003 and flanking linkers between Asp102 and Asp103

Depositor

Mark Howarth

Article

Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

Insert

sfGFP-SpyTag003 Loop B

Use

Tags

His6

Expression

Bacterial

Mutation

SpyTag003 and linkers inserted between residues A…

Promoter

T7

Available since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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KHBD00112

Plasmid

#34267

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG12223 (Dsp1 Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pGL4.10 Dub3

Plasmid

#72684

Purpose

luciferase reporter with Dub3 promoter

Depositor

Domenico Maiorano

Article

van der Laan et al PLoS One. 2014 Apr 2;9(4):e93663. doi: 1

Insert

Dub3 promoter (Usp17le Mouse)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Dub3

Available since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a-MKP7_wild_153

Plasmid

#37792

Depositor

SeWon Suh

Article

Kim et al Proc Natl Acad Sci U S A. 2012 May 15;10

Insert

Mitogen-activated protein kinase phosphatase 7 (DUSP16 Human)

Use

Tags

His

Expression

Bacterial

Mutation

deleted amino acids 154-665

Promoter

Available since

July 13, 2012

Availability

Academic Institutions and Nonprofits only
pET21a-MKP7_K55R_153

Plasmid

#37794

Depositor

SeWon Suh

Article

Kim et al Proc Natl Acad Sci U S A. 2012 May 15;10

Insert

Mitogen-activated protein kinase phosphatase 7 (DUSP16 Human)

Use

Tags

His

Expression

Bacterial

Mutation

changed Lysnie 55 to Arginine and deleted amino a…

Promoter

Available since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
pET21a-MKP7_K62R_153

Plasmid

#37795

Depositor

SeWon Suh

Article

Kim et al Proc Natl Acad Sci U S A. 2012 May 15;10

Insert

Mitogen-activated protein kinase phosphatase 7 (DUSP16 Human)

Use

Tags

His

Expression

Bacterial

Mutation

changed Lysnie 62 to Arginine and deleted amino a…

Promoter

Available since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
pET21a-MKP7_K52R_153

Plasmid

#37793

Depositor

SeWon Suh

Article

Kim et al Proc Natl Acad Sci U S A. 2012 May 15;10

Insert

Mitogen-activated protein kinase phosphatase 7 (DUSP16 Human)

Use

Tags

His

Expression

Bacterial

Mutation

changed Lysnie 52 to Arginine and deleted amino a…

Promoter

Available since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
p3xFLAG SARS-CoV2 RdRp.co

Plasmid

#154008

Purpose

Expression of codon-optimized SARS-CoV2 Nsp12 (RdRp) in mammalian cells

Depositor

Patrick Moore

Article

Meng et al J Med Virol. 2023 Jan;95(1):e28246. doi:

Insert

Codon-optimized SARS-CoV2 Nsp12 from ORF1ab (ORF1ab Synthetic)

Use

Tags

3xFLAG tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 23, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pYS104

Plasmid

#1190

Depositor

Susan Lindquist

Article

Patino et al Science 1996 Aug 2;273(5275):622-6.

Insert

Hsp104 (HSP104 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

wild-type Hsp104 sequence with 588bp of 5' p…

Promoter

Available since

June 23, 2005

Availability

Academic Institutions and Nonprofits only
pGEX-mUbp43-His

Plasmid

#12458

Depositor

Dong-Er Zhang

Article

Malakhov et al J Biol Chem. 2002 Mar 22. 277(12):9976-8

Insert

UBP43 (Usp18 Mouse)

Use

Tags

GST and His

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only
pCR1325

Plasmid

#111132

Purpose

Expresses USP1.

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

USP1 (USP1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pJC45BS104 Y819W

Plasmid

#1307

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 Y819W (HSP104 Budding Yeast)

Use

Tags

10X His tag

Expression

Bacterial

Mutation

Changed Tyr 819 to Trp

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pPS832

Plasmid

#1357

Depositor

Pamela Silver

Article

Schlenstedt et al Proc Natl Acad Sci U S A 1995 Jan 3;92(1

Insert

GSP1 (GSP1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only
pJC45BS104 L892W

Plasmid

#1308

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 L892W (HSP104 Budding Yeast)

Use

Tags

10X His tag

Expression

Bacterial

Mutation

Changed Leu 892 to Trp

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pJC45BS104 F130W

Plasmid

#1302

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 F130W (HSP104 Budding Yeast)

Use

Tags

10X His tag

Expression

Bacterial

Mutation

Changed Phe 130 to Trp

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pJC45BS104 L553W

Plasmid

#1306

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 L553W (HSP104 Budding Yeast)

Use

Tags

10X His tag

Expression

Bacterial

Mutation

Changed Leu 553 to Trp

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pVW293

Plasmid

#139490

Purpose

Insertion of the pHSP12 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiae

Depositor

Serge Pelet

Article

Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

Insert

pHSP12 24xPP7sl

Use

Tags

Expression

Yeast

Mutation

WT

Promoter

pHSP12

Available since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pPS834

Plasmid

#8932

Depositor

Pamela Silver

Article

Schlenstedt et al Proc Natl Acad Sci U S A 1995 Jan 3;92(1

Insert

GSP1 (GSP1 Budding Yeast)

Use

Tags

myc

Expression

Yeast

Mutation

Promoter

Available since

Aug. 19, 2005

Availability

Academic Institutions and Nonprofits only
pJC45BS104 Y257W

Plasmid

#1303

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 Y257W (HSP104 Budding Yeast)

Use

Tags

10X His tag

Expression

Bacterial

Mutation

Changed Tyr 257 to Trp

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge