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pmVenus(L68V)-mTurquoise2

Plasmid

#60493

Purpose

Produces a fusion between mTurquoise2 and the FRET acceptor mVenus(L68V). It can be used as a positive control for FRET from mTurquoise2 to mVenus(L68V)

Depositor

Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

SYFP2-mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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12XCSL-DsRedExpressDL

Plasmid

#47683

Purpose

Notch reporter

Depositor

Urban Lendahl

Article

Hansson et al Dev Neurosci. 2006;28(1-2):118-27.

Insert

TP-1 promoter

Use

Notch reporter construct

Tags

dsRedExpressDR

Mutation

hexamerized 50-bp EBNA2 response element of the T…

Promoter

NA

Available Since

Feb. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pAC93-pmax-dCas9VP160

Plasmid

#48225

Purpose

dCas9VP160 on pmax expression vector

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9(D10A;H840A) fusion with VP160 activation domain

Use

CRISPR

Tags

HA Tag and VP160

Expression

Mammalian

Mutation

D10A;H840A (catalytically inactive)

Promoter

CAGGS

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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tetO-GATA4

Plasmid

#46030

Depositor

John Gearhart

Article

Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

Insert

GATA4 (GATA4 Human)

Use

Lentiviral

Tags

His and V5

Expression

Mammalian

Mutation

R43Q, G50S and N197S

Promoter

tetO

Available Since

July 24, 2013

Availability

Academic Institutions and Nonprofits only

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CX-nestinGFPas

Plasmid

#32401

Depositor

Patrick Salmon, Henryk Zulewski

Article

Eberhardt et al Biochem Biophys Res Commun. 2006 Jul 7;3

Insert

nestin promoter/enhancer GFP

Use

Lentiviral

Available Since

June 19, 2013

Availability

Academic Institutions and Nonprofits only

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pLenti-MP2

Plasmid

#36097

Purpose

3rd generation lentiviral plasmid

Depositor

Pantelis Tsoulfas

Article

Enomoto et al Exp Neurol. 2013 Oct;248:170-82. doi: 10

Type

Empty backbone

Use

Lentiviral

Available Since

June 1, 2012

Availability

Academic Institutions and Nonprofits only

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pECE

Plasmid

#26453

Depositor

William Rutter

Article

Ellis et al Cell. 1986 Jun 6;45(5):721-32.

Type

Empty backbone

Expression

Mammalian

Available Since

Nov. 4, 2011

Availability

Academic Institutions and Nonprofits only

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pBMN YFP-FKBP(DD)

Plasmid

#31766

Depositor

Thomas Wandless

Article

Chu et al Bioorg Med Chem Lett. 2008 Nov 15;18(22)

Insert

HA-YFP-FKBP(DD) (FKBP1A Human)

Use

Retroviral

Tags

HA and YFP

Mutation

E31G, F36V, R71G and K105E on FKBP12

Promoter

LTR

Available Since

Oct. 4, 2011

Availability

Academic Institutions and Nonprofits only

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pGEM-HE-h_bPAC_cmyc

Plasmid

#28134

Purpose

optogenetic tool for light modulation of cellular cAMP

Depositor

Peter Hegemann

Article

Stierl et al J Biol Chem. 2011 Jan 14. 286(2):1181-8.

Insert

humanized photoactivated adenylyl cyclase

Use

In vitro transcription

Tags

Myc

Available Since

June 23, 2011

Availability

Academic Institutions and Nonprofits only

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pEFmEGFP-mDia2

Plasmid

#25407

Depositor

Arthur Alberts

Article

Tominaga et al Mol Cell. 2000 Jan . 5(1):13-25.

Insert

mDia2 (Diaph3 Mouse)

Tags

EGFP, His, and Myc

Expression

Mammalian

Mutation

Encodes mDia2

Available Since

Aug. 24, 2010

Availability

Academic Institutions and Nonprofits only

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