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pLKO-tet-puro-sh-ex16

Plasmid

#35167

Depositor

Benjamin Blencowe

Article

Gabut et al Cell. 2011 Sep 30;147(1):132-46. Epub 20

Insert

Foxp1 (Foxp1 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
pCMV-Flag-YAP-S109A

Plasmid

#33088

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

YAP (YAP1 )

Tags

Flag

Expression

Mammalian

Mutation

S109A

Promoter

CMV

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
3392 pcDNA3 Bid p15

Plasmid

#8808

Depositor

Stanley Korsmeyer

Article

Gross et al J Biol Chem 1999 Jan 8;274(2):1156-63.

Insert

Bid p15 (Bid Mouse)

Expression

Mammalian

Available Since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
pVRC8400-SARS-CoV-2-RBD-AVI

Plasmid

#160476

Purpose

Expresses SARS-CoV-2 RBD domain with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Zhou et al Cell Rep. 2020 Oct 27;33(4):108322. doi:

Insert

SARS-CoV-2-RBD

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Available Since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGGS SARS-CoV-2 BA.2 Spike

Plasmid

#185604

Purpose

Expresses codon-optimized full length SARS-CoV-2 BA.2 Spike

Depositor

Marceline Côté

Article

Tauzin et al Cell Rep. 2022 Oct 25;41(4):111554. doi:

Insert

SARS-CoV-2 BA.2 Spike (S )

Expression

Mammalian

Mutation

T19I, L24S, Δ25-27, G142D, V213G, G339D, S371F, S…

Promoter

chicken β-actin promoter, CMV enhancer

Available Since

July 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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AiP978-pAAV-mscRE4-minBGpromoter-FlpO-WPRE-hGHpA

Plasmid

#163472

Purpose

Direct-expressing FlpO AAV Virus. Alias: AiP978 - pAAV-AiE2004m-minBG-FlpO-WPRE-HGHpA

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

FlpO

Use

AAV

Promoter

Beta Globin minimal promoter

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_ATG16L1:1-607

Plasmid

#211234

Purpose

Mammalian expression of full-length human ATG16L1. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

ATG16L1:M1-Y607 (ATG16L1 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

T300A natural variant (VAR_021834)

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_NUP214:1-2090

Plasmid

#211220

Purpose

Mammalian expression of full-length human NUP214. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

NUP214:M1-S2090 (NUP214 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

P574S natural variant (VAR_045691). S1872N annot…

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_DCAF11:1-546

Plasmid

#211197

Purpose

Mammalian expression of full-length human DCAF11. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DCAF11:M1-Q546 (DCAF11 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

R207H natural variant (VAR_020121)

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_WDHD1:1-1129

Plasmid

#211175

Purpose

Mammalian expression of full-length human WDHD1. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDHD1:M1-E1129 (WDHD1 Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_WDR77:1-342

Plasmid

#211169

Purpose

Mammalian expression of full-length human WDR77. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR77:M1-E342 (WDR77 Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_EML4:1-981

Plasmid

#211315

Purpose

Mammalian expression of full-length human EML4. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

EML4:M1-S981 (EML4 Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

I382V natural variant (VAR_031727)

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pHTN-HaloTag_MTA2:1-668

Plasmid

#211016

Purpose

Mammalian expression of full-length human MTA2. N-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

MTA2:M1-D668 (MTA2 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pHTC-HaloTag_DDB1:1-1140

Plasmid

#211007

Purpose

Mammalian expression of full-length human DDB1. C-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DDB1:M1-H1140 (DDB1 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNLF1-N_FBXW7:1-707

Plasmid

#210946

Purpose

Mammalian expression of full-length human FBXW7. N-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

FBXW7:M1-K707 (FBXW7 Human)

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pTripZ RB1 WT-3XFLAG

Plasmid

#209070

Purpose

DOX-ON inducible expression vector that expresses RB1 WT with c-terminal 3X flag tag

Depositor

Matthew Oser

Article

Oser et al Cancer Discov. 2019 Feb;9(2):230-247. do

Insert

human RB1 WT after LR recombination with pTripZ NEO DEST to inducibly express RB1 WT with a c-terminal 3X flag tag

Use

Lentiviral

Tags

3XFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pMG207

Plasmid

#39273

Depositor

Peter Kast

Article

Gamper et al Biotechniques. 2005 Mar;38(3):405-8.

Type

Empty backbone

Use

E coli gene targeting

Available Since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pPlin5-mScarlet3-H (aka mYongHong)

Plasmid

#239408

Purpose

Expresses Plin5-mScarlet3-H in mammalian cells

Depositor

Zhifei Fu

Article

Xiong et al Nat Methods. 2025 Apr 17. doi: 10.1038/s

Insert

Plin5 (PLIN5 Human)

Tags

mScarlet3-H (aka mYongHong)

Expression

Mammalian

Mutation

M163H in mScarlet3

Promoter

CMV

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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miniTol2 x EF1a_EKAREN5 + PGK-puro

Plasmid

#167817

Purpose

Optimized EKAREV FRET biosensor sensor for ERK

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN5

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W

Promoter

EF1a

Available Since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223_TUBB_WT

Plasmid

#81990

Purpose

Gateway Donor vector containing TUBB, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

TUBB (TUBB Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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