We narrowed to 17,786 results for: Met;

1. pCFJ914

   Plasmid

   #44489

   Depositor

   Erik Jorgensen

   Article

   Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

   Insert

   Minimal Mos1 [Peft-3 GFP H2B tbb-2 UTR, NeoR]
   

   Use

   Gateway expression clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBabe-SIRT3

   Plasmid

   #66167

   Purpose

   Expresses SIRT3 via retroviral method

   Depositor

   Ronald Kahn

   Article

   Jing et al Diabetes. 2013 Oct;62(10):3404-17. doi:

   Insert

   SIRT3 (Sirt3 Mouse)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   L235P

   Promoter

   Available since

   July 18, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-Zif268

   Plasmid

   #69224

   Purpose

   Expresses R1335K mutant SpCas9 fused to Zif268 in mammalian cell

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   NLS-3XHA-NLS and Zif268

   Expression

   Mammalian

   Mutation

   Arginine 1335 is changed to Lysine

   Promoter

   Available since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. fgfprom luc

   Plasmid

   #69446

   Purpose

   basal plasmid containing minimal fgf4 promoter used for testing enhancer activity of DNAs inserted immediately upstream of the promoter.

   Depositor

   Lisa Dailey

   Article

   Murtha et al Nat Methods. 2014 May;11(5):559-65. doi:

   Insert

   minimal fgf4 promoter
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. mIFP-MannII-N-10

   Plasmid

   #56229

   Purpose

   Localization: Mannosidase/Golgi, Excitation: 683, Emission: 704

   Depositor

   Michael Davidson

   Article

   Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

   Insert

   MannII
   

   Use

   Tags

   mIFP

   Expression

   Mammalian

   Mutation

   aa 1-112 of MannII

   Promoter

   CMV

   Available since

   April 28, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

6. pBEST-HipO

   Plasmid

   #114599

   Purpose

   Strong constitutive expression of metabolic enzyme, HipO, for cell-free expression

   Depositor

   Jerome Bonnet

   Article

   Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

   Insert

   HipO
   

   Use

   Synthetic Biology; Cell-free protein synthesis

   Tags

   Expression

   Mutation

   Promoter

   OR2-OR1-Pr

   Available since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMSP1D1T1

   Plasmid

   #97327

   Purpose

   Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional T residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.

   Depositor

   Michael Marty

   Article

   Reid et al Anal Chem. 2017 Nov 7;89(21):11189-11192

   Insert

   MSP1D1T1
   

   Use

   Tags

   6xHis - TEV

   Expression

   Bacterial

   Mutation

   Addition of T residue after T25

   Promoter

   T7

   Available since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. TALEN-CCR5L18-CtermCan

   Plasmid

   #51438

   Purpose

   Expresses TALEN targeting left CCR5A site (L18) with canonical C-terminal domain

   Depositor

   David Liu

   Article

   Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

   Insert

   TALEN CCR5 Left (L18) Can C-term
   

   Use

   TALEN

   Tags

   3xFLAG and Fok1 EL variant

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. TALEN-CCR5R18-CtermCan

   Plasmid

   #51439

   Purpose

   Expresses TALEN targeting right CCR5A site (R18) with canonical C-terminal domain

   Depositor

   David Liu

   Article

   Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

   Insert

   TALEN CCR5 Right (R18) Can C-term
   

   Use

   TALEN

   Tags

   3xFLAG and Fok1 KK variant

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCSDest2-SpCas9-MT3-NLS-3XHA-NLS

    Plasmid

    #69223

    Purpose

    Expresses R1335K mutant SpCas9 in mammalian cell

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    NLS-3XHA-NLS

    Expression

    Mammalian

    Mutation

    Arginine 1335 is changed to Lysine

    Promoter

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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