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pColE1_sgRNA_pos2_AmpR

Plasmid

#119145

Purpose

Encodes sgRNA targeting position 2 in pColE1_70a_deGFP_KanR

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 2 in pColE1_70a_deGFP_KanR

Use

Crispr

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pIA1402

Plasmid

#172403

Purpose

Expression of SARS-COV-2 Nsp12 Y129A in E. coli

Depositor

Irina Artsimovitch

Article

Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

Insert

SARS-COV-2 Nsp12 (ORF1ab Synthetic)

Tags

His-SUMO

Expression

Bacterial

Mutation

Y129A

Promoter

T7

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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Septin 11 isoform IV

Plasmid

#120320

Purpose

Septin 11 isoform IV mammalian expression

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 (Sept11 Rat)

Expression

Mammalian

Available Since

Jan. 24, 2019

Availability

Academic Institutions and Nonprofits only

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Septin 11 isoform I

Plasmid

#120314

Purpose

Septin 11 isoform I mammalian expression

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 (Sept11 Rat)

Expression

Mammalian

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pFRR5

Plasmid

#167506

Purpose

Broad-Host range expression vector, RSF1010 origin, RhaR-RhaS activator, PrhaBAD promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

RhaS-RhaR-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PrhaBAD

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pUHD-SB-mAID/Bla

Plasmid

#171680

Purpose

TRE-controlled mAID-fusion sleeping beauty transposon vector with selection marker (blasticidin)

Depositor

Randy Poon

Article

Yeung et al Mol Biol Cell. 2021 May 12:mbcE21020051.

Type

Empty backbone

Tags

mAID

Expression

Mammalian

Promoter

TRE

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pNH2

Plasmid

#40853

Depositor

Adam Bogdanove

Article

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

repeat NH2

Available Since

Dec. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pJUMP41-2A(sfGFP)

Plasmid

#126985

Purpose

Level 2 vector. sfGFP reporter. Origin R6K (pir conditional).

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pIA1408

Plasmid

#172405

Purpose

Expression of SARS-COV-2 Nsp12 D760A in E. coli

Depositor

Irina Artsimovitch

Article

Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

Insert

SARS-COV-2 Nsp12 (ORF1ab Synthetic)

Tags

His-SUMO

Expression

Bacterial

Mutation

D760A

Promoter

T7

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pFRG5

Plasmid

#149486

Purpose

Broad-Host range expression vector, RSF1010 origin, RhaR-RhaS activator, PrhaBAD promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

RhaS-RhaR-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PrhaBAD

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pFA6a-GBP-RFP-hphMX6

Plasmid

#105161

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Davidson et al Mol Biol Cell. 2015 Feb 1;26(3):453-66.

Insert

GBP-RFP-hphMX6

Tags

GBP-RFP

Expression

Bacterial and Yeast

Available Since

Feb. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pJES102

Plasmid

#135409

Purpose

E. coli 'blaTEM-1 (without signal sequence) that can be used as a reporter

Depositor

Miriam Braunstein

Article

McCann et al Microbiology. 2007 Oct;153(Pt 10):3350-9

Insert

blaTEM-1

Expression

Bacterial

Promoter

Unknown

Available Since

Feb. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pBY2727

Plasmid

#35399

Depositor

Ralf Baumeister

Article

Schaffer et al Nucleic Acids Res. 2010 Apr;38(6):e91. d

Insert

SnAvi-tag

Use

Mouse Targeting

Expression

Mammalian

Promoter

CMV

Available Since

April 9, 2012

Availability

Academic Institutions and Nonprofits only

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pGMEH-P38-luxAB2

Plasmid

#27061

Depositor

Dirk Schnappinger

Article

Kim et al Nucleic Acids Res. 2011 Mar;39(6):2210-2

Insert

P#38-luxAB

Expression

Bacterial

Available Since

May 11, 2011

Availability

Academic Institutions and Nonprofits only

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pNH3

Plasmid

#40854

Depositor

Adam Bogdanove

Article

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

repeat NH3

Available Since

Dec. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pNH4

Plasmid

#40855

Depositor

Adam Bogdanove

Article

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

repeat NH4

Available Since

Dec. 6, 2012

Availability

Academic Institutions and Nonprofits only

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BASIC_SEVA_5a6.10

Plasmid

#178934

Purpose

BASIC DNA assembly vector

Depositor

Paul Freemont

Article

Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

Insert

mScarlet counter-selection cassette

Use

Synthetic Biology

Available Since

Feb. 7, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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TOPO-BC43

Plasmid

#84437

Purpose

Encodes illumina barcodes for allele frequency sequencing (FREQ-Seq-Squared)

Depositor

J.J. Emerson

Article

Zhao et al G3 (Bethesda). 2023 Sep 30;13(10):jkad16

Insert

BC43

Use

Barcoding plasmids

Promoter

SP6/T7

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pNH9

Plasmid

#40860

Depositor

Adam Bogdanove

Article

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

repeat NH9

Available Since

Dec. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pBY2946

Plasmid

#23223

Depositor

Ralf Baumeister

Article

Schaffer et al Nucleic Acids Res. 2010 Apr;38(6):e91. d

Insert

SnAvi

Expression

Worm

Available Since

May 4, 2010

Availability

Academic Institutions and Nonprofits only

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