We narrowed to 19,300 results for: Mut

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pJWK_VD_03

Plasmid

#158104

Purpose

CaV1.2 5'UTR luciferase reporter

Depositor

Ted Dawson

Article

Kim et al Cell Stem Cell. 2020 Oct 1;27(4):633-645

Insert

5'UTR of CACNA1C (uc009zdu.1)

Use

Luciferase

Expression

Mammalian

Promoter

PGK

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-VprBP (N909)

Plasmid

#133588

Purpose

Expresses human VprBP N909 mutant in mammalian cells

Depositor

Yue Xiong

Article

Nakagawa et al Mol Cell. 2015 Jan 22;57(2):247-60. doi:

Insert

Vpr (HIV-1) binding protein (DCAF1 Human)

Tags

HA

Expression

Mammalian

Mutation

C590 mutation of human VprBP lacking the DDB1-bin…

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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MEN2A-MAC

Plasmid

#139634

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

Insert

MEN2A (RET Human)

Tags

StrepIII-HA-BirA*

Expression

Mammalian

Mutation

C634W

Promoter

CMV

Available Since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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GRB2-SHN

Plasmid

#139625

Purpose

StrepIII-HA-tagged gene expression

Depositor

Markku Varjosalo

Article

Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

Insert

GRB2 (GRB2 Human)

Tags

StrepIII-HA

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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BtGRK2[D110A K220R]-mVenus

Plasmid

#137779

Purpose

Visualization of GRK2

Depositor

Dorus Gadella

Article

Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

Insert

Bovine GRK2[D110A K220R] (GRK2 Bovine)

Expression

Mammalian

Mutation

D110A K220R

Promoter

CMV

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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HsGRK6[L66A E514A]-sYFP2

Plasmid

#137781

Purpose

Visualization of GRK6

Depositor

Dorus Gadella

Article

Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

Insert

Human GRK6[L66A E514A] (GRK6 Human)

Expression

Mammalian

Mutation

L66A E514A

Promoter

CMV

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pAWp63-clone3-12 (OptiHF-SpCas9)

Plasmid

#131737

Purpose

Expresses OptiHF-SpCas9 in mammalian cells

Depositor

Alan Siu Lun Wong

Article

Choi et al Nat Methods. 2019 Aug;16(8):722-730. doi

Insert

OptiHF-SpCas9

Use

Lentiviral

Tags

Flag

Mutation

Q695A+K848A+E923M+T924V+Q926A

Promoter

EFS

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pMinDis.X513-iNicSnFR3b-(V7)-ER

Plasmid

#125123

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotine

Depositor

Henry Lester

Article

Shivange et al J Gen Physiol. 2019 Feb 4. pii: jgp.2018

Insert

iNicSnFR3a-(V7)-ER

Tags

Fusion protein -Endoplasmic retention motif

Expression

Mammalian

Promoter

T7

Available Since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pTAMAHISTEV_PfeAR480A

Plasmid

#128944

Purpose

expresses R480A mutant of PfeA in E. coli

Depositor

James Naismith

Article

Moynie et al Nat Commun. 2019 Aug 14;10(1):3673. doi:

Insert

ferric enterobactin receptor

Tags

TEV protease cleavable 7xHis and TamA

Expression

Bacterial

Mutation

Signal peptide sequence has been removed (1-75), …

Promoter

T7

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pTAMAHISTEV_PfeAG324V

Plasmid

#128947

Purpose

expresses G324V mutant of PfeA in E. coli

Depositor

James Naismith

Article

Moynie et al Nat Commun. 2019 Aug 14;10(1):3673. doi:

Insert

ferric enterobactin receptor

Tags

TEV protease cleavable 7xHis and TamA

Expression

Bacterial

Mutation

Signal peptide sequence has been removed (1-75), …

Promoter

T7

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pBADHISTEV_PfeEH258A

Plasmid

#129122

Purpose

expresses H258A mutant of PfeE in E.coli

Depositor

James Naismith

Article

Perraud et al ACS Chem Biol. 2018 Sep 21;13(9):2603-26

Insert

Periplasmic Esterase PfeE (PA2689 Pseudomonas aeruginosa PAO1)

Tags

TEV protease cleavable 6xHis

Expression

Bacterial

Mutation

Signal peptide sequence has been removed, mutat…

Promoter

PBAD

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pBADHISTEV_PfeES157A

Plasmid

#129121

Purpose

expresses S157A mutant of PfeE in E.coli

Depositor

James Naismith

Article

Perraud et al ACS Chem Biol. 2018 Sep 21;13(9):2603-26

Insert

Periplasmic Esterase PfeE (PA2689 Pseudomonas aeruginosa PAO1)

Tags

TEV protease cleavable 6xHis

Expression

Bacterial

Mutation

Signal peptide sequence has been removed, mutat…

Promoter

PBAD

Available Since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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PHAGE-P CMVt N-HA hE6-AP II (AA 1-87)

Plasmid

#119317

Purpose

lentiviral vector for expression of HA-Tagged E6-AP I AA 1-87 in mammalian cells

Depositor

Peter Howley

Article

Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

Insert

E6-AP II (UBE3A Human)

Use

Lentiviral

Tags

HA

Mutation

Only AA 1-87

Promoter

CMV

Available Since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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PHAGE-P CMVt N-HA hE6-AP I (AA 1-64)

Plasmid

#119310

Purpose

lentiviral vector for expression of HA-Tagged E6-AP I AA 1-64 in mammalian cells

Depositor

Peter Howley

Article

Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

Insert

E6-AP I (UBE3A Human)

Use

Lentiviral

Tags

HA

Mutation

Only AA 1-64

Promoter

CMV

Available Since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

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pLC-Flag-SENP8 sg2R-P2A-Hygro

Plasmid

#124300

Purpose

Lentiviral vector for expression of Flag tagged SENP8-P2A-Hygro casette from a CMV promoter. SENP8 cDNA contains silent mutations to disrupt a sgRNA binding site.

Depositor

Eva Gottwein

Article

Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

Insert

SENP8 (SENP8 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

several silent mutations to disrupt sgRNA targeti…

Promoter

CMV

Available Since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pHHM.X513-iNicSnFR3a-(CC93)

Plasmid

#124880

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotine

Depositor

Henry Lester

Article

Shivange et al J Gen Physiol. 2019 Feb 4. pii: jgp.2018

Insert

iNicSnFR3a-(CC93)

Tags

HA Tag and His Tag

Expression

Bacterial

Mutation

NA

Promoter

T7

Available Since

May 28, 2019

Availability

Academic Institutions and Nonprofits only

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pHHM.X513-iNicSnFR2-(CC90)

Plasmid

#124836

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotine

Depositor

Henry Lester

Article

Shivange et al J Gen Physiol. 2019 Feb 4. pii: jgp.2018

Insert

iNicSnFR2-(CC90)

Tags

HA tags and His Tag

Expression

Bacterial

Mutation

NA

Promoter

T7

Available Since

May 28, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-deltaA1-R1597W-VWF

Plasmid

#124826

Purpose

Expresses multimeric human VWF protein without A1 domain and a R1597W mutation in A2 domain. This protein has C-terminal c-Myc tag.

Depositor

Sriram Neelamegham

Article

Zhang et al Blood Adv. 2019 Apr 9;3(7):957-968. doi:

Insert

VWF without A1 domain and R1597W mutation A2 domain (VWF Human)

Expression

Mammalian

Promoter

CMV

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-deltaA1-D1498A-VWF

Plasmid

#124825

Purpose

Expresses multimeric human VWF protein without A1 domain and a D1498A mutation in A2 domain. This protein has C-terminal c-Myc tag.

Depositor

Sriram Neelamegham

Article

Zhang et al Blood Adv. 2019 Apr 9;3(7):957-968. doi:

Insert

VWF without A1 domain and D1498A mutation A2 domain (VWF Human)

Expression

Mammalian

Promoter

CMV

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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pCEP4-001428(753)-VC

Plasmid

#123256

Purpose

Mammalian expression plasmid for Env from the 001428 HIV-1 isolate; C-terminal truncation fused to the C-terminal half of split fluorescent Venus (VC)

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (001428) Env

Tags

CD5 leader peptide and VC (Venus residues D155–K2…

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; C-terminal Env re…

Promoter

CMV

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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