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pME-NLStdMCP- tagRFP

Plasmid

#86244

Purpose

Middle entry vector for inserting promotors for tissue specific expression of NLS-tdMCP-tagRFP

Depositor

Florence Marlow

Article

Campbell et al Development. 2015 Apr 1;142(7):1368-74.

Type

Empty backbone

Use

Unspecified

Tags

NLS-tdMCP-tagRFP

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pBAD-pHoran4

Plasmid

#61558

Purpose

Orange fluorescent protein based pH indicator in bacterial expression vector

Depositor

Robert Campbell

Article

Shen et al J Cell Biol. 2014 Nov 10;207(3):419-32.

Insert

pHoran4

Tags

6xHis

Expression

Bacterial

Available Since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only
pRVdG-1mOrange2-5PSD95EGFP

Plasmid

#52482

Purpose

Expresses mOrange2 and PSD-95-EGFP

Depositor

Sebastian Seung

Article

Wickersham et al Nat Commun. 2013;4:2332. doi: 10.1038/nc

Inserts

mOrange2
PSD-95-EGFP

Use

Rabies virus

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

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pQE-WT-CGG-R12-KIR

Plasmid

#63626

Purpose

CGG-R12-KIR RNAP mutant

Depositor

Andrew Ellington

Article

Meyer et al ACS Synth Biol. 2014 Oct 7.

Insert

K1F IKR RNAP

Mutation

Q744K, L747V, N748H, L749I, R756E, L757M, H772R

Promoter

T5/Lac

Available Since

July 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pUPD2-HA-LRR-NELC492A-C (B3-B4)

Plasmid

#211862

Purpose

Mutant E3-DART ligase in domestication vector

Depositor

Marcela Rojas-Pierce

Article

Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

Insert

LRR-NEL

Use

Synthetic Biology

Tags

HA

Mutation

C492A

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pMXs-Ecat8

Plasmid

#13357

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

ES cell associated transcript 8 (Tdrd12 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pQE-WT-N4-R5-RNRV

Plasmid

#63630

Purpose

N4-R5-RNRV RNAP mutant

Depositor

Andrew Ellington

Article

Meyer et al ACS Synth Biol. 2014 Oct 7.

Insert

N4-R5-RNRV RNAP

Mutation

L747I, N748D, L749C, M750V, F751I, Q754T, F755R, …

Promoter

T5/Lac

Available Since

April 15, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pMT Draper-mCherry

Plasmid

#126667

Purpose

Inducible expression of Draper-mCherry in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Draper mCherry

Expression

Insect

Mutation

C813Y- see depositor comments below

Available Since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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pTZ U6+27

Plasmid

#25713

Purpose

for nuclear expression of small RNAs in mammalian cells

Depositor

David Engelke

Article

Good et al Gene Ther. 1997 Jan . 4(1):45-54.

Insert

U6 snRNP (RNU6-1 Human)

Expression

Mammalian

Available Since

June 24, 2010

Availability

Academic Institutions and Nonprofits only
pCG-B-SpyTag-C

Plasmid

#87373

Purpose

An ORF part-containing vector harbouring the SpyTag sequence with 4 bp overhangs specific for the ‘upstream SpyPart’ position within an ORF assembly

Depositor

Tom Ellis

Article

Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021

Insert

SpyTag

Use

Synthetic Biology

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pQE-WT-KIF-R5-KIKR

Plasmid

#63624

Purpose

KIF-R5-KIKR RNAP mutant

Depositor

Andrew Ellington

Article

Meyer et al ACS Synth Biol. 2014 Oct 7.

Insert

KIF-R5-KIKR RNAP

Mutation

Q744K, L749I, M750K, Q754S, R756N, I761V, H772R

Promoter

T5/Lac

Available Since

April 15, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pET15b-eIF4E

Plasmid

#37228

Depositor

Jon Lorsch

Article

Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

Insert

eIF4E

Expression

Bacterial

Promoter

T7

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
pTH07

Plasmid

#70063

Purpose

artificial transcription template "distal pause"

Depositor

Jeff Gelles

Article

Harden et al Proc Natl Acad Sci U S A. 2016 Jan 5. pi

Insert

lambda PR' - repeat cassette - E. coli rpoB with inserted pause - lambda TR'

Available Since

Jan. 7, 2016

Availability

Academic Institutions and Nonprofits only
pTH09

Plasmid

#70064

Purpose

artificial transcription template "terminator insertion"

Depositor

Jeff Gelles

Article

Harden et al Proc Natl Acad Sci U S A. 2016 Jan 5. pi

Insert

lambda PR' - lambda TR2 - repeat cassette - E. coli rpoB - lambda TR'

Available Since

Jan. 7, 2016

Availability

Academic Institutions and Nonprofits only
pNHA9

Plasmid

#223642

Purpose

pUC18 plasmid with rpsM promoter

Depositor

François-Xavier Campbell-Valois

Article

Haidar-Ahmad et al mSphere. 2024 Nov 21;9(11):e0055324. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pUPD2-LRR-NEL (B4-B5)

Plasmid

#211859

Purpose

CDS for E3-DART ligase in domestication vector

Depositor

Marcela Rojas-Pierce

Article

Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

Insert

LRR-NEL

Use

Synthetic Biology

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pFH2.18_TagBFP

Plasmid

#205533

Purpose

Express TagBFP

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

TagBFP

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pWSK29-wzzpHS2

Plasmid

#172971

Purpose

pWSK29 with S. flexneri wzzpHS2 gene

Depositor

Renato Morona

Article

Murray et al J Bacteriol. 2006 Apr;188(7):2735-9. doi

Insert

wzzPHS2 gene from Shigella flexneri

Expression

Bacterial

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCAFNF-EGFP

Plasmid

#163609

Purpose

Expresses EGFP Flp-dependently in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Egfp

Expression

Mammalian

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pWSK29-wzzBSF

Plasmid

#172967

Purpose

pWSK29 with S. flexneri 2a wzzBSF gene

Depositor

Renato Morona

Article

Murray et al J Bacteriol. 2006 Apr;188(7):2735-9. doi

Insert

wzzB gene from Shigella flexneri

Expression

Bacterial

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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