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pILGFP88

Plasmid

#83544

Purpose

Used to evaluate the expression output of RPL15A promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

RPL15A promoter-yEGFP

Expression

Yeast

Promoter

RPL15A

Available Since

Dec. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pILGFP77

Plasmid

#83538

Purpose

Used to evaluate the expression output of RPL8B promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

RPL8B promoter-yEGFP

Expression

Yeast

Promoter

RPL8B

Available Since

Dec. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pILGFPH0

Plasmid

#83552

Purpose

Used to evaluate the expression output of TPS1 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

TPS1 promoter-yEGFP

Expression

Yeast

Promoter

TPS1

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pILGFP90

Plasmid

#83547

Purpose

Used to evaluate the expression output of TEF2 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

TEF2 promoter-yEGFP

Expression

Yeast

Promoter

TEF2

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pILGFP82

Plasmid

#83541

Purpose

Used to evaluate the expression output of YEF3 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

YEF3 promoter-yEGFP

Expression

Yeast

Promoter

YEF3

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pILGFP80

Plasmid

#83540

Purpose

Used to evaluate the expression output of RPL3promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

RPL3 promoter-yEGFP

Expression

Yeast

Promoter

RPL3

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pILGFP78

Plasmid

#83539

Purpose

Used to evaluate the expression output of SSB1 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

SSB1 promoter-yEGFP

Expression

Yeast

Promoter

SSB1

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pET21-MRPL13

Plasmid

#31344

Depositor

Linda Spremulli

Article

Emdadul Haque et al Mitochondrion. 2008 Jun . 8(3):254-61.

Insert

mitochondrial ribosomal protein L13 mature form (MRPL13 Human)

Tags

His

Expression

Bacterial

Mutation

Mature form after removal of the predicted mitoch…

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
mycUb-20aa

Plasmid

#20255

Depositor

Nico Dantuma

Article

Verhoef et al FASEB J. 2009 Jan . 23(1):123-33.

Insert

Ubiquitin-20 amino acid extension (RBX1 Human)

Tags

myc

Expression

Mammalian

Mutation

Ubiquitin fused to N-terminus of GFP. Glycine 76 …

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only

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B1-INTEGRIN VN

Plasmid

#195216

Purpose

Mammalian expression of mCherry tagged B1 Integrin. Autoclustering mutant.

Depositor

David Bryant, Valerie Weaver

Article

Paszek et al Cancer Cell. 2005 Sep;8(3):241-54. doi:

Insert

ITGB1 (ITGB1 Human)

Expression

Mammalian

Mutation

V737N

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-TetOne-Puro-LUC

Plasmid

#218630

Purpose

This plasmid contains luciferase under doxycycline inducible control.

Depositor

Valentin Cracan

Article

Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038

Insert

LUC (LOC116160065 )

Use

Lentiviral, Luciferase, and Synthetic Biology

Expression

Mammalian

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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SpyDock

Plasmid

#124618

Purpose

Expresses SpyDock to bind SpyTag non-covalently for affinity purification

Depositor

Mark Howarth

Article

Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

Insert

SpyDock

Tags

His6

Expression

Bacterial

Mutation

E77A mutation prevents isopeptide bond formation,…

Promoter

T7

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pDblet

Plasmid

#8848

Depositor

Joel Huberman

Article

Brun et al Gene. 1995 Oct 16. 164(1):173-7.

Insert

ARS doublet

Use

Yeast cloning vector

Expression

Yeast

Mutation

This vector contains, between AatII sites, a dire…

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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pSEMS-SDHB-Halo7Tag

Plasmid

#108973

Purpose

Selflabeling tag at succinate dehydrogenase complex subunit B (SDHB)

Depositor

Karin Busch

Article

Wilkens et al J Cell Sci. 2013 Jan 1;126(Pt 1):103-16.

Insert

succinate dehydrogenase complex iron sulfur subunit B (SDHB Human)

Tags

Halo7-tag

Expression

Mammalian

Promoter

CMV

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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piggyBAC-H2B FR-MQV

Plasmid

#194079

Purpose

Expresses nuclear-localized FR-MQV in mammalian cells

Depositor

Ralph Jimenez

Article

Mukherjee et al Biochemistry. 2020 Oct 6;59(39):3669-368

Insert

FR-MQV

Tags

Histone H2B

Expression

Mammalian

Mutation

Methionine 42 to Glutamine, Cystiene 159 to Valin…

Promoter

CMV7

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pV1524

Plasmid

#111431

Purpose

CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Mal2-FLP)

Depositor

Gerald Fink

Article

Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

Insert

CaCas9/sgRNA-BsmBI stuffer

Use

CRISPR

Expression

Yeast

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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VCP(wt)-EGFP

Plasmid

#23971

Depositor

Nico Dantuma

Article

Tresse et al Autophagy. 2010 Feb 22. 6(2):.

Insert

Valosin containing protein (VCP Human)

Tags

6xHis, EGFP, and HA

Expression

Mammalian

Mutation

none

Available Since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only

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B1-INTEGRIN WT

Plasmid

#195215

Purpose

Mammalian expression of mCherry tagged B1 Integrin. Wild-type.

Depositor

David Bryant, Valerie Weaver

Article

Paszek et al Cancer Cell. 2005 Sep;8(3):241-54. doi:

Insert

ITGB1 (ITGB1 Human)

Expression

Mammalian

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pBabe_Hyg_cOVA_T2A_mStrawberry

Plasmid

#161737

Purpose

Expression of cOVA and mStrawberry

Depositor

Alec Kimmelman

Article

Yamamoto et al Nature. 2020 May;581(7806):100-105. doi:

Insert

cytoplasmic ovalbumin

Use

Retroviral

Tags

mStrawberry

Expression

Mammalian

Available Since

Dec. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pBabe_Zeo_cOVA_T2A_mStrawberry

Plasmid

#161736

Purpose

Expression of cOVA and mStrawberry

Depositor

Alec Kimmelman

Article

Yamamoto et al Nature. 2020 May;581(7806):100-105. doi:

Insert

cytoplasmic ovalbumin

Use

Retroviral

Tags

mStrawberry

Expression

Mammalian

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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