We narrowed to 8,431 results for: BLI

1. pT5T_Rev7_1-211_SA112_KVK_Rev3_1989-2014

   Plasmid

   #105637

   Purpose

   Expresses mouse monomeric Rev7 and fragment of Rev3 protein (amino acids 1989-2014) from bicistronic plasmid

   Depositor

   Thanos Halazonetis

   Inserts

   Rev7 (Mad2l2 Mouse)
   Rev3l (REV3L Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Mutations: F11S, G12A, V132K, C133V, A135K

   Promoter

   T7

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS2+WBP2NL-HA

   Plasmid

   #174791

   Purpose

   Xenopus laevis WBP2NL with 3'HA tag

   Depositor

   Sally Moody

   Article

   Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

   Insert

   WBP2 N-terminal like (wbp2nl.L Frog)
   

   Use

   Tags

   HA

   Expression

   Bacterial

   Mutation

   aa#165, Y to H; aa# 287, D to H

   Promoter

   SP6

   Available since

   Dec. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENTR4 CDS A. thaliana PARP1

   Plasmid

   #175818

   Purpose

   pENTR4 plasmid with CDS of Arabidopsis thaliana POLY(ADP-RIBOSE) POLYMERASE 1 (PARP1, AT2G31320.1) without stop codon for C-terminal epitope tags

   Depositor

   Lennart Wirthmueller

   Insert

   PARP1 (PARP1 Mustard Weed)
   

   Use

   Pentr plasmid for gateway cloning

   Tags

   none

   Expression

   Mutation

   one silent mutation, annotated in GenBank file

   Promoter

   none

   Available since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCS2+-wbp2nl

   Plasmid

   #172463

   Purpose

   expresses wild-type protein when injected into embryos

   Depositor

   Sally Moody

   Article

   Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

   Insert

   wbp2nl (wbp2nl.L Frog)
   

   Use

   Xenopus expression

   Tags

   none

   Expression

   Mutation

   none

   Promoter

   SP6

   Available since

   Sept. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pB6-Id1-loxP-AU1-rox-FNF-loxP-drePBD*-rox

   Plasmid

   #168770

   Purpose

   B6J Id1 AU1 tag and LSL-TagRFP-T-drePBD* targeting vector, low copy number

   Depositor

   Mario Capecchi

   Insert

   Id1 (Id1 Mouse)
   

   Use

   Cre/Lox and Mouse Targeting; Dre / rox

   Tags

   Expression

   Mutation

   See Depositor Comments Below

   Promoter

   Available since

   Aug. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pB6-Cdk6-T2A-TagRFPT-dre-PBD*-FNF-TK

   Plasmid

   #141452

   Purpose

   B6J Cdk6-T2A-TagRFPT-dre-PBD* allele targeting vector, low copy number

   Depositor

   Mario Capecchi

   Insert

   Cdk6 (Cdk6 Mouse)
   

   Use

   Mouse Targeting; Dre / rox

   Tags

   C-terminal fusion of T2A-TagRFPT-dre-PBD*

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. psiCHECK2 3'UTR GAC

   Plasmid

   #110401

   Purpose

   Luminescence-based glutaminase GAC isoform 3'UTR-mediated RNA stability reporter

   Depositor

   Sandra Martha Gomes Dias

   Insert

   GLS glutaminase (GLS Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC19 KGA HA5'-mKO2-3'

   Plasmid

   #110406

   Purpose

   Homology donor for glutaminase KGA isoform knock-in, mKO2

   Depositor

   Sandra Martha Gomes Dias

   Insert

   GLS glutaminase (GLS Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFA-DAB1

   Plasmid

   #162691

   Purpose

   Expresses DabAB1 inorganic carbon transport complex

   Depositor

   David Savage

   Article

   Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

   Insert

   DabAB1 inorganic carbon transport complex
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PLtet0-1 promoter

   Available since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. mPLD2-PX (62-192)

    Plasmid

    #119128

    Purpose

    Bacterial expression of human phox homology (PX) domain, mPLD2-PX (62-192)

    Depositor

    Brett Collins

    Article

    Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

    Insert

    mPLD2-PX (62-192)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    April 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFE-dabAB2 C539A

    Plasmid

    #133008

    Purpose

    pFE carrying a dabA2 gene (Uniprot: D0KWS7) with a C539A mutation and a dabB2 gene  (Uniprot: D0KWS8)

    Depositor

    David Savage

    Article

    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

    Inserts

    dabB2
    dabA2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C539A

    Promoter

    tetR

    Available since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFE-dabAB2 H524A

    Plasmid

    #133007

    Purpose

    pFE carrying a dabA2 gene (Uniprot: D0KWS7) with a H524A mutation and a dabB2 gene (Uniprot: D0KWS8)

    Depositor

    David Savage

    Article

    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

    Inserts

    dabB2
    dabA2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    H524A

    Promoter

    tetR

    Available since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFE-dabAB2 D353A

    Plasmid

    #133006

    Purpose

    pFE carrying a dabA2 gene (Uniprot: D0KWS7) with a D353A mutation and a dabB2 gene  (Uniprot: D0KWS8)

    Depositor

    David Savage

    Article

    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

    Inserts

    dabB2
    dabA2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D353A

    Promoter

    tetR

    Available since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCSD_FKBP-C-SpCas9MT3-574-1368-NLS-3XHA-NLS-ZFPTS3-3xFLAG-2xNLS

    Plasmid

    #107300

    Purpose

    Expresses C terminal (574-1368 aa) R1335K mutant SpCas9 fused to FKBP and ZFP-VEGFA-TS3 in mammalian cells

    Depositor

    Scot Wolfe

    Insert

    Truncated SpCas9 (aa 574-1368) fused to FKBP
    

    Use

    CRISPR

    Tags

    3x Flag, 2x NLS, FKBP, SV40NLS, 3xHA, c-Myc NLS, …

    Expression

    Mammalian

    Mutation

    Truncated SpCas9 (aa 574-1368) with R1335K mutati…

    Promoter

    CMV IE94

    Available since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCSD_FKBP-C-SpCas9MT3-574-1368-NLS-3XHA-NLS-ZFPTS2-3xFLAG-2xNLS

    Plasmid

    #107299

    Purpose

    Expresses C terminal (574-1368 aa) R1335K mutant SpCas9 fused to FKBP and ZFP-VEGFA-TS2 in mammalian cells

    Depositor

    Scot Wolfe

    Insert

    Truncated SpCas9 (aa 574-1368) fused to FKBP
    

    Use

    CRISPR

    Tags

    3x Flag, 2x NLS, FKBP, SV40NLS, 3xHA, c-Myc NLS, …

    Expression

    Mammalian

    Mutation

    Truncated SpCas9 (aa 574-1368) with R1335K mutati…

    Promoter

    CMV IE94

    Available since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. Halo-Tev-Akt3 C119S-2X Flag

    Plasmid

    #87608

    Purpose

    expresses halo tagged at the N-terminus and 2 tandem flag tags at the C-terminus of C119S mutant of human Akt3 protein

    Depositor

    Yimon Aye

    Insert

    AKT3 isoform 2 (AKT3 Human)
    

    Use

    Zebrafish (danio rerio) expression

    Tags

    2 tandem flag tags and His-Halo-Tev

    Expression

    Mammalian

    Mutation

    cysteine 119 residue mutated to serine

    Promoter

    Available since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. 4xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7 probasin_mTQ2_FlpO

    Plasmid

    #68356

    Purpose

    -The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex7 and SMAD4 ex2. are expressed by the U6 promoter

    Depositor

    Jannik Elverløv-Jakobsen

    Inserts

    4xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7
    FlpO recombinase
    

    Use

    CRISPR

    Tags

    mTurquoise2 (BFP)

    Expression

    Mammalian

    Mutation

    Promoter

    Synthetic Probasin ARRx2 and U6

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGS1623

    Plasmid

    #63795

    Purpose

    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal tDimer2 Twin-Strep tag followed by HIS5 selection marker

    Depositor

    Monika Golas, Bjoern Sander

    Article

    Rai et al Mol Biotechnol. 2014 Jun 27.

    Insert

    tDimer2-Twin-Strep tag followed by HIS5
    

    Use

    E. coli cloning vector

    Tags

    tDimer2-Twin-Strep

    Expression

    Mutation

    Promoter

    none

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGS1322

    Plasmid

    #63792

    Purpose

    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eCFP Twin-Strep tag followed by URA3 selection marker

    Depositor

    Monika Golas, Bjoern Sander

    Article

    Rai et al Mol Biotechnol. 2014 Jun 27.

    Insert

    eCFP-Twin-tag followed by URA3
    

    Use

    E. coli cloning vector

    Tags

    eCFP-Twin-tag

    Expression

    Mutation

    Promoter

    none

    Available since

    April 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGS1112

    Plasmid

    #63784

    Purpose

    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eSapphire Strep-tag II followed by URA3 selection marker

    Depositor

    Monika Golas, Bjoern Sander

    Article

    Rai et al Mol Biotechnol. 2014 Jun 27.

    Insert

    eSapphire-Strep-tag II followed by URA3
    

    Use

    E. coli cloning vector

    Tags

    Sapphire-Strep-tag II

    Expression

    Mutation

    Promoter

    none

    Available since

    April 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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