We narrowed to 22,696 results for: Sis;

1. pCI-TPI_WT-xrRNA-4H

   Plasmid

   #108368

   Purpose

   Expresses wild type TPI reporter; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)

   Depositor

   Niels Gehring

   Article

   Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.

   Insert

   Wild type TPI reporter with MVE xrRNA and 4H probe binding sites (TPI1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGoldyTALEN-TUBA1B-TAL-2

   Plasmid

   #62854

   Purpose

   TALEN mediated gene editing at human TUBA1B locus

   Depositor

   Gaudenz Danuser

   Article

   Gan et al Cell Syst. 2016 Sep 21. pii: S2405-4712(

   Insert

   TALEN array
   

   Use

   TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   miniCAGG

   Available Since

   May 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGoldyTALEN-TUBA1B-TAL-1

   Plasmid

   #62853

   Purpose

   TALEN mediated gene editing at human TUBA1B locus

   Depositor

   Gaudenz Danuser

   Article

   Gan et al Cell Syst. 2016 Sep 21. pii: S2405-4712(

   Insert

   TALEN array
   

   Use

   TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   miniCAGG

   Available Since

   May 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJMP1055

   Plasmid

   #119242

   Purpose

   construction intermediate

   Depositor

   Oren Rosenberg, Jason Peters, Carol Gross

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   sgRNA NT1/RR1 (rfp)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCI-FLAG-mTUT4(D1028A)

   Plasmid

   #60220

   Purpose

   Mammalian expression of FLAG-tagged catalytic mutant of TUT4

   Depositor

   Zissimos Mourelatos

   Article

   Liu et al Mol Cell. 2014 Aug 27. pii: S1097-2765(1

   Insert

   TUT4 (Tut4 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D1028A

   Promoter

   CMV

   Available Since

   Dec. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pMSCV-PIG-miR-18a

   Plasmid

   #64228

   Purpose

   Expresses miR-18a in mammalian cells

   Depositor

   Joshua Mendell

   Article

   Chang et al Nat Genet. 2008 Jan;40(1):43-50. Epub 20

   Insert

   miR-18a (MIR18A Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPKm-118

   Plasmid

   #90491

   Purpose

   Expresses pcDNA3 - CMVmin - 5X UAS - pFR Luciferase

   Depositor

   Todd Coleman

   Article

   Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

   Insert

   pFR Luciferase Fluc
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. PhyB-mCherry-SIK1

   Plasmid

   #51577

   Purpose

   Constitutively express nucleolus localized PhyB in Saccharomyces cerevisiae

   Depositor

   Chao Tang

   Article

   Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

   Insert

   PhyB (PHYB Mustard Weed)
   

   Use

   Tags

   SIK1 and mCherry

   Expression

   Yeast

   Mutation

   1-908aa only

   Promoter

   ADH1pr

   Available Since

   Aug. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. p148

   Plasmid

   #62616

   Purpose

   ePathBrick pETM6 vector encoding CsF3H, AaDFR, and DuLAR

   Depositor

   Mattheos Koffas

   Article

   Zhao et al Metab Eng. 2014 Dec 17;28C:43-53. doi: 1

   Inserts

   flavaone 3B-hydroxylase
   dihydroflavonol 4-reductase
   leucoanthocyanidin reductase
   

   Use

   Synthetic Biology

   Tags

   GBD, PDZ, and SH3

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. PH-miRFP-FRB

    Plasmid

    #193568

    Purpose

    Expresses PH domain from PLC delta1 tagged with miRFP703 and FRB mutant (T2098L) in mammalian cells.

    Depositor

    Justin Taraska

    Article

    Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

    Insert

    PH domain from PLC delta1 (Plcd1 Rat)
    

    Use

    Tags

    FRB (T2098L) and miRFP703

    Expression

    Mammalian

    Mutation

    PH domain

    Promoter

    CMV

    Available Since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. Ef1a-QCP-3xBFP

    Plasmid

    #158206

    Purpose

    Qb coat protein fused to three repeats of the blue fluorescent protein under a Efla promoter.

    Depositor

    Roee Amit

    Article

    Katz et al Nat Commun. 2021 Mar 11;12(1):1576. doi:

    Insert

    QCP under the Elfa promoter and three BFP tags
    

    Use

    Synthetic Biology

    Tags

    3xBFP

    Expression

    Mammalian

    Mutation

    Promoter

    Elfa

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. p6896 PHAGE-P CMVT N-HA-hE6AP II wt

    Plasmid

    #37603

    Depositor

    Peter Howley

    Article

    Martinez-Noel et al Mol Cell Biol. 2012 May 29.

    Insert

    E6AP II wt (UBE3A Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV tet regulatable

    Available Since

    Aug. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. Halo-WIPI2B H85E

    Plasmid

    #175027

    Purpose

    Expresses WIPI2B (H85E) fused to Halo in mammalian cells to abrogate interaction with ATG16L1

    Depositor

    Andrea Stavoe

    Article

    Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

    Insert

    WIPI2B (H85E) (WIPI2 Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Histadine 85 to Glutamate (CAC to GAG)

    Promoter

    CMV

    Available Since

    Dec. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. UKNP1.21.2

    Plasmid

    #98200

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pUAST mito roGFP2-Orp1

    Plasmid

    #64997

    Purpose

    for expression of mitochondrial roGFP2-Orp1 with the Gal4 expression system in drosophila

    Depositor

    Tobias Dick

    Article

    Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

    Insert

    Orp1 (HYR1 Budding Yeast)
    

    Use

    Tags

    roGFP2

    Expression

    Insect

    Mutation

    mitochondrial targeting sequence

    Promoter

    Available Since

    June 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEF HA-TSAdwt

    Plasmid

    #84517

    Purpose

    Mammalian expression of TSAd

    Depositor

    Anne Spurkland

    Article

    Granum et al BMC Immunol. 2006 Jul 13;7:15.

    Insert

    TSAd (SH2D2A Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pLJM1-CRY2-mCherry-(GGGGS)3-hRGS2box

    Plasmid

    #112257

    Purpose

    Expresses RGS component of opto-RGS2 in mammalian cells

    Depositor

    Brian Chow

    Article

    Hannanta-Anan et al ACS Synth Biol. 2018 Jun 15;7(6):1488-14

    Insert

    CRY2-mCherry-(GGGGS)3-hRGS2box
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET-HJURP-CBP

    Plasmid

    #30393

    Depositor

    Yawen Bai

    Article

    Zhou et al Nature. 2011 Apr 14. 472(7342):234-7.

    Insert

    HJURP CenpA Binding Domain (CENPA Budding Yeast, Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 11, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA-HA-hTRAF3 88-C

    Plasmid

    #44034

    Depositor

    Shao-Cong Sun

    Article

    Liao et al J Biol Chem. 2004 Jun 18;279(25):26243-5

    Insert

    TNF receptor-associated factor 3 (TRAF3 Human)
    

    Use

    Tags

    1xHA

    Expression

    Mammalian

    Mutation

    Contains amino acids 89-568; V274D and S393G

    Promoter

    Available Since

    March 27, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pCI-neo-mAtg12

    Plasmid

    #22870

    Depositor

    Noboru Mizushima

    Article

    Mizushima et al FEBS Lett. 2002 Dec 18. 532(3):450-4.

    Insert

    autophagy-related genes 12 (Atg12 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 25, 2010

    Availability

    Academic Institutions and Nonprofits only

21. pCDH-CAG-IntCCCre-EF1a-Cerulean

    Plasmid

    #160508

    Purpose

    Expresses IntCCCre in mammalian cells in split intein-mediated split-Cre recombinase applications

    Depositor

    Shawn Je

    Article

    Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

    Insert

    IntCCCre
    

    Use

    Cre/Lox and Lentiviral

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Jan. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBI-CMV1-mir181b-1-CMV2-mir1

    Plasmid

    #164670

    Purpose

    Express miR-181b-1 and miR-1-1 in mammalian cells

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Inserts

    miR-181b-1 (MIR181B1 Human)
    miR-1-1 (MIR1-1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET23a-MmRPS27a

    Plasmid

    #69559

    Purpose

    Bacterial expression; in vitro transcription/translation (T7)

    Depositor

    Jorge Eduardo Azevedo

    Article

    Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

    Insert

    RPS27A (RPS27A Human, Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Changed Alanine 133 (human RPS27a) to Glycine (mo…

    Promoter

    T7

    Available Since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

24. PhyB-mCherry-SNF7

    Plasmid

    #51574

    Purpose

    Constitutively express endosome localized PhyB in Saccharomyces cerevisiae

    Depositor

    Chao Tang

    Article

    Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

    Insert

    PhyB (PHYB Mustard Weed)
    

    Use

    Tags

    SNF7 and mCherry

    Expression

    Yeast

    Mutation

    1-908aa only

    Promoter

    ADH1pr

    Available Since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCI-globin_del5UTR_PTC39-xrRNA-4H

    Plasmid

    #108367

    Purpose

    Expresses beta-globin reporter with premature termination codon (PTC39); enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)

    Depositor

    Niels Gehring

    Article

    Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.

    Insert

    PTC39 beta-globin reporter with MVE xrRNA and 4H probe binding sites
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    PTC39

    Promoter

    CMV

    Available Since

    May 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pHH0103_STAC3-2/2

    Plasmid

    #91466

    Purpose

    Protein expression and purification of human SH3 domain construct STAC3-2/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    STAC3-2/2 (STAC3 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHH0103_SH3GL1-1/1

    Plasmid

    #91473

    Purpose

    Protein expression and purification of human SH3 domain construct SH3GL1-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    SH3GL1-1/1 (SH3GL1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHH0103_VAV2-1/2*

    Plasmid

    #91483

    Purpose

    Protein expression and purification of human SH3 domain construct VAV2-1/2*

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    VAV2-1/2* (VAV2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pHH0103_STAC3-1/2

    Plasmid

    #91271

    Purpose

    Protein expression and purification of human SH3 domain construct STAC3-1/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    STAC3-1/2 (STAC3 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. UKNP1.19.2

    Plasmid

    #98193

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge