Addgene

We narrowed to 16,452 results for: EMB

Showing: 7321 - 7340 of 16452 results

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pFP.R400

Plasmid

#138175

Purpose

Expresses the split mCherry reporter interrupted by the chimeric Npu/SspDnaE intein (in cis)

Depositor

Baojun Wang

Article

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
mCherryN(GGK).NpuSsp*.(CWN)mCherryC.H6

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

P(araBAD) and iPC

Available since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAc-sgRNA-Cas9.scr_2

Plasmid

#190704

Purpose

Expresses scrambled sgRNA and Cas9-Puro in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

Scrambled sgRNA 2

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Drosophila U6

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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TRF2-HA

Plasmid

#200209

Purpose

Expression of full length mTRF2

Depositor

Sheila Teves

Article

Kwan et al Elife. 2023 Mar 30;12:e83810. doi: 10.75

Insert

TRF2-HA (Terf2 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

None

Promoter

EF_1alpha

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pFA6a-3GFP-kanMX6

Plasmid

#105148

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu, Thomas Pollard, David Kovar

Article

Wu et al J Cell Biol. 2006 Jul 31;174(3):391-402.

Insert

3GFP-kanMX6

Use

Tags

3GFP

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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Sox1/2/3-2373/+3>nls::Cas9::nls

Plasmid

#59989

Purpose

Sox1/2/3 (SoxB1) driver driving nls::Cas9::nls

Depositor

Lionel Christiaen

Article

Stolfi et al Development. 2014 Nov;141(21):4115-20. d

Insert

nls::Cas9::nls

Use

CRISPR

Tags

Expression

Mutation

Reverted mutations from dCas9 to wiltdtype

Promoter

Ciinte.Sox1/2/3 (SoxB1) -2373/+3

Available since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P14

Plasmid

#120025

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mNeptune2.5

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mNeptune2.5

Use

Pcr template

Tags

mNeptune2.5

Expression

Mutation

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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Col1A-5'probe

Plasmid

#20732

Depositor

Rudolf Jaenisch

Article

Beard et al Genesis. 2006 Jan . 44(1):23-8.

Insert

Col1A-5'probe (Col1a1 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 24, 2009

Availability

Academic Institutions and Nonprofits only

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pMS31: pTwinStrep-SUMO-PmcTnsAB

Plasmid

#168164

Purpose

PmcTnsAB expression construct for protein purification.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Insert

PmcCAST TnsAB

Use

Tags

6X His, Twin Strep and SUMO

Expression

Bacterial

Mutation

Promoter

Available since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z15

Plasmid

#120058

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with miRFP670

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

miRFP670

Use

Pcr template

Tags

miRFP670

Expression

Mutation

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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TOPO CCSP

Plasmid

#68453

Purpose

TOPO construct expressing CCSP for generation of GMAP-compatible promoter

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Clara Cell Secretory Protein Promoter

Use

Gmap

Tags

Expression

Bacterial

Mutation

Promoter

Plac

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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Tol2_B-act_dCas9-Vp64-T2A-Citrine

Plasmid

#200248

Purpose

Zebrafish transgenics; Plasmid encodes dCas9-Vp64 and could be stably integrated in the zebrafish genome using Tol2 transgenesis

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Insert

dCas9-Vp64-T2A-Citrine

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Available since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pR540

Plasmid

#207885

Purpose

template for 2 gRNA amplification

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

scaffold-pU6

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCS021

Plasmid

#207917

Purpose

Level 0 for gRNA in E2 module (5' CCAT-3' ACTA)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

pU6-AarI-scaffold-term

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pKE013

Plasmid

#207916

Purpose

Level 0 for gRNA in E1 module (5' CTGC-3' CCAT)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

pU3-AarI-scaffold-term

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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OMM PKA Reporter

Plasmid

#104550

Purpose

Expresses Tom20-mTurquoise2-VASP at the outer mitochondrial membrane of mammalian cells.

Depositor

David Lawrence

Article

O'Banion et al Cell Chem Biol. 2017 Oct 25. pii: S2451-

Insert

Tom20 - mTurquoise2 - VASP 148-164

Use

Tags

mTurqouise2

Expression

Mammalian

Mutation

Promoter

EF1 alpha

Available since

March 6, 2018

Availability

Academic Institutions and Nonprofits only

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pET15b+PCNA1 P186C

Plasmid

#66173

Purpose

Expresses the P186C mutant of Sulfolobus solfataricus PCNA1

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 1

Use

Tags

His6 tag

Expression

Bacterial

Mutation

P186C

Promoter

T7 promoter

Available since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P15

Plasmid

#120026

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with miRFP670

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

miRFP670

Use

Pcr template

Tags

miRFP670

Expression

Mutation

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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Cyto-RA

Plasmid

#209860

Purpose

Dimerization dependent fluorescent protein RA fused to NES

Depositor

Sarah Cohen

Article

Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

Insert

ddRFP A

Use

Tags

NES sequence NLVDLQKKLEELELDEQQ

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pTPI1-EMC1-GFP-APEX2-URA3

Plasmid

#184776

Purpose

Expresses EMC1-GFP-APEX2 chimera for labeling yeast endoplasmic reticulum (ER). Uses auxotrophic marker URA3(Kluyveromyces lactis).

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

APEX2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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TAL2385

Plasmid

#36794

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

BRCA1-TALEN_Right (Brca1 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Expression

Mutation

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only