We narrowed to 8,688 results for: App

1. pBabe-Puro-Myr-Flag-AKT1

   Plasmid

   #15294

   Depositor

   William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   AKT1 (AKT1 Human)
   

   Use

   Retroviral

   Tags

   Myr-Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. PX458-3xHA-en1FnCas9

   Plasmid

   #201947

   Purpose

   Mammalian expression plasmid of enhanced activity FnCas9 variant en1FnCas9 with T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

   Insert

   3xHA-NLS-en1FnCas9-T2A-EGFP
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   E1369R on FnCas9

   Promoter

   Cbh

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV x EF1a_EKAREN4-ires-puro

   Plasmid

   #167828

   Purpose

   Optimized EKAREV FRET biosensor sensor for ERK

   Depositor

   Hugo Snippert

   Article

   Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

   Insert

   EKAREN4
   

   Use

   Lentiviral

   Tags

   nls localization motif

   Expression

   Mammalian

   Mutation

   K424P, K426W

   Promoter

   EF1a

   Available since

   April 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pWZL Neo Myr Flag PKM2

   Plasmid

   #20585

   Depositor

   Jean Zhao, William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   PKM2 (PKM Human)
   

   Use

   Retroviral

   Tags

   Flag and Myr

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDH-MSCV-hCD38-EF1α-GFP+Puro

   Plasmid

   #134936

   Purpose

   expression human CD38 in mammalian cells

   Depositor

   Jianjun Zhao

   Article

   Hu et al Cancer Res. 2020 Mar 19. pii: 0008-5472.

   Insert

   CD38 (CD38 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET30(a)-AtNUDX23

   Plasmid

   #175021

   Purpose

   Purification of AtNUDX23 enzyme

   Depositor

   Jeppe Vinther

   Article

   Sherwood et al Nature. 2023 Jul;619(7971):811-818. doi:

   Insert

   AtNUDX23 (NUDX23 Mustard Weed)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR223 SARS-CoV-2 NSP12

   Plasmid

   #141265

   Purpose

   Gateway-compatible Entry vector, with insert of NSP12 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   NSP12 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Gene ID 43740578)
   

   Use

   Gateway-compatible entry vector

   Tags

   Expression

   Mutation

   Many synonymous changes due to codon optimization

   Promoter

   Available since

   April 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. CS-TRE-BMI1-Ubc-rtTA

   Plasmid

   #198191

   Purpose

   Generation of Expandable megakaryocyte cell lines from human iPS cells

   Depositor

   Hiromitsu Nakauchi

   Article

   Nakamura et al Cell Stem Cell. 2014 Apr 3;14(4):535-48.

   Insert

   hBMI1 (BMI1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pWZL Neo Myr Flag PIK4CA

   Plasmid

   #20577

   Depositor

   Jean Zhao, William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   PIK4CA (PI4KA Human)
   

   Use

   Retroviral

   Tags

   Flag and Myr

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA5-FRT/TO-3*Flag-APEX2 N-term FBL

    Plasmid

    #187577

    Purpose

    Express FLAG epitope and APEX2-tagged FBL fusion protein in mammalian cells

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Insert

    FBL (FBL Human)
    

    Use

    Tags

    APEX2-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFB-EPCP1

    Plasmid

    #226783

    Purpose

    Expression of Z-tagged LRRK2 RCKW using Baculoviruses

    Depositor

    Sebastian Mathea

    Article

    Dederer et al J Biol Chem. 2024 Jul;300(7):107469. doi

    Insert

    Leucine-rich repeat kinase 2 (LRRK2 Human)
    

    Use

    Tags

    His-Z-TEV

    Expression

    Insect

    Mutation

    Amino acids deleted 1-1326

    Promoter

    Available since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV x EF1a_EKAREN5(TA)-ires-puro

    Plasmid

    #167823

    Purpose

    control sensor for EKAREN5

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN5(TA)
    

    Use

    Lentiviral

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W, T420A

    Promoter

    EF1a

    Available since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA5-FRT/TO-3*Flag-APEX2 C-term EWSR1

    Plasmid

    #187586

    Purpose

    Express FLAG epitope and APEX2-tagged EWSR1 fusion protein in mammalian cells

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Insert

    EWSR1 (EWSR1 Human)
    

    Use

    Tags

    APEX2-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pWZL Neo Myr Flag PKN2

    Plasmid

    #20587

    Depositor

    Jean Zhao, William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    PKN2 (PKN2 Human)
    

    Use

    Retroviral

    Tags

    Flag and Myr

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

15. pDONR221-GATA6

    Plasmid

    #101418

    Purpose

    Donor Vector containing GATA6 transcription factor, part of the Human TFome Collection

    Depositor

    George Church

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    GATA6 (GATA6 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Expression

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Promoter

    Available since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. N-Terminal Split Cas9 D10A Nickase with GyrA intein

    Plasmid

    #58695

    Purpose

    Expresses N-terminus of D10A SpCas9 nickase domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 nickase production

    Depositor

    Gang Bao

    Article

    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103

    Insert

    D10A Nickase humanized S. pyogenes Cas9 with Gyra Nsplit Intein
    

    Use

    AAV and CRISPR

    Tags

    GyrA Nsplit Intein, HA Tag, and NLS

    Expression

    Mammalian

    Mutation

    D10A nickase converting mutation to SpCas9

    Promoter

    CBh

    Available since

    Sept. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBabe-Puro-MEK-DD

    Plasmid

    #15268

    Depositor

    William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    Map2k1 (Mapk1 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    S218D S222D

    Promoter

    Available since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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18. pWZL Neo Myr Flag PRKAA1

    Plasmid

    #20595

    Depositor

    Jean Zhao, William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    PRKAA1 (PRKAA1 Human)
    

    Use

    Retroviral

    Tags

    Flag and Myr

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

19. CS-TRE-c-Myc-Ubc-rtTA

    Plasmid

    #198190

    Purpose

    Generation of Expandable megakaryocyte cell lines from human iPS cells

    Depositor

    Hiromitsu Nakauchi

    Article

    Nakamura et al Cell Stem Cell. 2014 Apr 3;14(4):535-48.

    Insert

    hC-MYC (MYC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDNA5-LRRK2

    Plasmid

    #229019

    Purpose

    Expression of untagged full length human LRRK2 in mammalian cells

    Depositor

    Samara Reck-Peterson

    Article

    Dederer et al J Biol Chem. 2024 Jul;300(7):107469. doi

    Insert

    Leucine-rich repeat kinase 2 (LRRK2 Human)
    

    Use

    Tags

    no tags (untagged)

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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