We narrowed to 9,404 results for: pho;

1. pcDNA3.3−3xFLAG−AGO2(S387A)

   Plasmid

   #220462

   Purpose

   Express S387 phospho-null mutant human AGO2 with N-terminal 3xFLAG tag in mammalian cells

   Depositor

   David Bartel

   Article

   Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

   Insert

   Human AGO2 (AGO2 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Serine 387 to alanine

   Promoter

   CMV

   Available Since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. p3F-Strep-mBCL10

   Plasmid

   #33314

   Depositor

   Vanessa Redecke

   Article

   Stempin et al J Biol Chem. 2011 Oct 28;286(43):37147-5

   Insert

   Bcl10 (Bcl10 Mouse)
   

   Tags

   3xFLAG and StrepOne

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Dec. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pEB1N-mScarlet-LZ-LOV2

   Plasmid

   #197419

   Purpose

   Mammalian expression of the mScarlet-tagged N-terminal half of photo-inactivated π-EB1

   Depositor

   Torsten Wittmann

   Article

   Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

   Insert

   MAPRE1 (MAPRE1 Human)
   

   Tags

   A. sativa phototropin 1 LOV2 domain, GCN4 leucine…

   Expression

   Mammalian

   Mutation

   EB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…

   Available Since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLC-CUL4A K705R-P2A-Puro

   Plasmid

   #124305

   Purpose

   Lentiviral vector for expression of Nedd8 attachment mutant K705R CUL4A-P2A-Puro casette from a CMV promoter

   Depositor

   Eva Gottwein

   Article

   Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

   Insert

   CUL4A (CUL4A Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   changed Lysine 705 to Arginine

   Promoter

   CMV

   Available Since

   July 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. M3-PSCFP2

   Plasmid

   #114185

   Purpose

   studying clustering of M3 receptors by superresolution microscopy (PALM)

   Depositor

   Aleksandra Radenovic

   Article

   Scarselli et al J Biol Chem. 2012 May 11;287(20):16768-8

   Insert

   Muscarin acetylcholine receptor 3 (CHRM3 Human)
   

   Tags

   3xHA and PS-CFP2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. LUC2 (L/Sec/deltaP)

   Plasmid

   #112145

   Purpose

   Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity

   Depositor

   Paul Copeland

   Article

   Mehta et al J Biol Chem. 2004 Sep 3;279(36):37852-9.

   Insert

   Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and deleted AUGA in PHGPx SECIS core (Gpx4 Photinus pyralis, Rat)
   

   Expression

   Mammalian

   Mutation

   Cysteine 258 mutated to selenocysteine (U) and d…

   Available Since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. XE237 Human LEF-1 pCS2+

   Plasmid

   #16709

   Depositor

   Randall Moon

   Article

   Li et al J Biol Chem. 2004 Jan 23;279(4):2873-84.

   Insert

   Human LEF-1 (LEF1 Human)
   

   Use

   Xenopus expression

   Available Since

   March 14, 2008

   Availability

   Academic Institutions and Nonprofits only

8. pCSCRET2(#323)

   Plasmid

   #184058

   Purpose

   cyclofen-inducible CRE activation via mammalian cell transfection or mRNA synthesis

   Depositor

   Sophie Vriz

   Article

   Sinha et al Zebrafish. 2010 Jun;7(2):199-204. doi: 1

   Insert

   6myc-CRE-ERT2
   

   Use

   Synthetic Biology

   Tags

   6xMyc

   Expression

   Mammalian

   Mutation

   ERT2: G400V, M543A, L544A, deleted 1-281;

   Promoter

   sCMV+SP6

   Available Since

   July 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-ALK2 K235R

   Plasmid

   #80872

   Purpose

   mammalian expression of ALK2 K235R

   Depositor

   Aristidis Moustakas

   Article

   Raja et al Oncotarget. 2016 Jan 12;7(2):1120-43. do

   Insert

   ALK2 (ACVR1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K235R

   Promoter

   CMV

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pTET-IUPi

    Plasmid

    #122019

    Purpose

    Expresses IUP pathway genes (ChK, IPK, idi) under inducible promoter. aTC inducible. Kan. resist.

    Depositor

    Gregory Stephanopoulos

    Article

    Chatzivasileiou et al Proc Natl Acad Sci U S A. 2018 Dec 24. p

    Inserts

    Choline kinase (CKI1 Budding Yeast)
    Isopentenyl monophosphate kinase (AT1G26640 Mustard Weed)
    isopentenyl-diphosphate Delta-isomerase
    

    Expression

    Bacterial

    Mutation

    Codon optimized for E. coli

    Promoter

    pTET

    Available Since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNMSB99

    Plasmid

    #199308

    Purpose

    MosSCI insertion of myo-3p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChR2-HDRC::SL2::jRGECO1a::let-858 3'UTR at ttTi5605

    Depositor

    Michael Krieg

    Article

    Porta-de-la-Riva et al Nat Methods. 2023 Apr 6. doi: 10.1038/s4

    Insert

    myo-3p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChR2-HDRC::SL2::jRGECO1a::let-858 3'UTR
    

    Expression

    Worm

    Mutation

    mTagBFP2 from pJJR81, ChR2(H134R;D156C), C. elega…

    Promoter

    myo-3

    Available Since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTIGER_mNeonGreen::3xFLAG::dCrk

    Plasmid

    #131137

    Purpose

    UASp construct for over-expression of Drosophila Crk with N-terminal monomeric NeonGreen and 3X FLAG tag. Compatible with PhiC31-mediated site-specific integration or classical P-element insertion.

    Depositor

    Mark Peifer

    Article

    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

    Insert

    Crk (Crk Fly)
    

    Tags

    3XFLAG and mNeonGreen

    Expression

    Insect

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBabe RFP-DN-FOXO1-HA neo

    Plasmid

    #45813

    Depositor

    Kevin Janes

    Article

    Wang et al Proc Natl Acad Sci U S A. 2011 Oct 4;108

    Insert

    FOXO1 (FOXO1 Human)
    

    Use

    Retroviral

    Tags

    HA and RFP

    Expression

    Mammalian

    Mutation

    aa1-255

    Available Since

    June 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. LUC45 (L/Sec/mutP)

    Plasmid

    #112148

    Purpose

    Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity

    Depositor

    Paul Copeland

    Article

    Mehta et al J Biol Chem. 2004 Sep 3;279(36):37852-9.

    Insert

    Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and MUTANT AUGA PHGPx SECIS core (Gpx4 Photinus pyralis, Rat)
    

    Expression

    Mammalian

    Mutation

    Cysteine 258 mutated to selenocysteine (U) and SE…

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTH728-CEN-RLuc/maxCFLuc

    Plasmid

    #38212

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Inserts

    Firefly Luciferase
    Renilla luciferase
    

    Expression

    Yeast

    Mutation

    All codons of the original FLuc gene were exchang…

    Promoter

    ADH1 and TDH3 (=GPD)

    Available Since

    Oct. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. CIP2Aprom1802bp-pGL4.10Luc

    Plasmid

    #60868

    Purpose

    Luc reporter driven by 1802 bp of the CIP2A promoter

    Depositor

    Jukka Westermarck

    Article

    Khanna et al PLoS One. 2011 Mar 22;6(3):e17979. doi:

    Insert

    1802 bp promoter fragment of Cancerous Inhibitor of PP2A (CIP2A Human)
    

    Use

    Luciferase; Promoterless

    Available Since

    April 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMRX-IP-hWIPI1A-3×FLAG

    Plasmid

    #215509

    Purpose

    Expresses FLAG tagged human WIPI1A.

    Depositor

    Noboru Mizushima

    Article

    Shimizu et al Hum Mol Genet. 2023 Aug 7;32(16):2623-26

    Insert

    WD-repeat protein interacting with phosphoinositides 1A (WIPI1 Human)
    

    Use

    Retroviral

    Tags

    3×FLAG

    Expression

    Mammalian

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPICZ-mouseGIRK2-eGFP-StII-6xHis

    Plasmid

    #124277

    Purpose

    Expresses mouse GIRK2 as a TEV protease cleavable C-terminal fusion to eGFP, strep tag II, and 6x His tag

    Depositor

    Arthur Laganowsky

    Article

    Liu et al Nat Commun. 2019 Mar 22;10(1):1352. doi:

    Insert

    GIRK2 (Kcnj6 Mouse)
    

    Tags

    eGFP-StreptagII-6xHis

    Expression

    Yeast

    Promoter

    AOX1

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV-tetO-Id2

    Plasmid

    #70764

    Purpose

    Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Id2

    Depositor

    Hubert Schorle

    Article

    Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

    Insert

    inhibitor of DNA binding 2 (Id2 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    tetO

    Available Since

    Nov. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3-mCherry-TSC2(AA,3Q)

    Plasmid

    #192444

    Purpose

    Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A, K1614Q, K1615Q, R1616Q mutations to disrupt Akt phosphorylation and GAP activity.

    Depositor

    Jin Zhang

    Article

    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

    Insert

    mCherry-TSC2(S939A,T1439A,K1614Q,K1615Q,R1616Q ) (TSC2 Human)
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …

    Promoter

    CMV

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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