We narrowed to 10,337 results for: plasmids 101

1. pAAV CMV-DIO-(mCherry-U6)-shRNA(anti-Crh)

   Plasmid

   #214732

   Purpose

   Expresses shRNA against CRH, marked with mCherry, in infected and cre positive cells

   Depositor

   Félix Leroy

   Article

   de Leon Reyes et al Cell. 2023 Sep 14;186(19):4152-4171.e31.

   Insert

   anti-Crh shRNA / mCherry
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-CAG-DIO-ChroME2f-ST-P2A-H2B-mRuby3

   Plasmid

   #171150

   Purpose

   Cation channelrhodopsin ChroME2f targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

   Depositor

   Hillel Adesnik

   Article

   Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

   Insert

   ChroME2f-P2A-H2B-mRuby3
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAS_4xEcoLeuT(CUA)_AnapRS

   Plasmid

   #140019

   Purpose

   Plasmid with 4xEcoLeuT(CUA) cassette and E. coli AnapRS for amber suppression and incorporation of the fluorescent ncAA Anap; for transient or stable piggyBac-mediated integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

   Insert

   EcoLeuRS (AnapRS)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   L38F, M40G, L41P, Y499V, Y500L, Y527A, H537E, L53…

   Promoter

   EF1

   Available Since

   May 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-C3-Sec5

   Plasmid

   #53756

   Purpose

   pEGFP-C3 plasmid that expresses Sec5

   Depositor

   Channing Der

   Article

   Martin et al Mol Cell. 2014 Jan 23;53(2):209-20. doi:

   Insert

   Sec5 (EXOC2 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 29, 2014

   Availability

   Academic Institutions and Nonprofits only

5. AAV-Syn-DIO-TRPV1-P2A-mCherry

   Plasmid

   #200831

   Purpose

   In the presence of Cre, drives neuron-specific expression of TRPV1 with fluorescence reporter mCherry

   Depositor

   Hong Chen

   Article

   Yang et al Brain Stimul. 2021 Jul-Aug;14(4):790-800

   Insert

   TRPV1-P2A-mCherry (Trpv1 Rat)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available Since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-C3-Sec15

   Plasmid

   #53760

   Purpose

   pEGFP-C3 plasmid that expresses Sec15

   Depositor

   Channing Der

   Article

   Martin et al Mol Cell. 2014 Jan 23;53(2):209-20. doi:

   Insert

   Sec15 (EXOC6 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 1, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pET-EIN-AlkBH5(66-292)

   Plasmid

   #125732

   Purpose

   Plasmid with EIN as a solubility and expression enhancing tag to increase production of hard to express proteins such as AlkBH5 and TEV cleaving site for the removal of the fusion tag.

   Depositor

   Vincenzo Venditti

   Article

   Khatiwada et al Protein Expr Purif. 2019 Nov 15;167:1055

   Insert

   AlkBH5(66-292) (ALKBH5 Human)
   

   Use

   Tags

   6xHis and EIN

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-C3-Sec8

   Plasmid

   #53758

   Purpose

   pEGFP-C3 plasmid that expresses Sec8

   Depositor

   Channing Der

   Article

   Martin et al Mol Cell. 2014 Jan 23;53(2):209-20. doi:

   Insert

   Sec8 (EXOC4 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 1, 2014

   Availability

   Academic Institutions and Nonprofits only

9. AAV-SYP1-miniSOG-T2A-mCherry

   Plasmid

   #50972

   Purpose

   AAV2 transfer vector containing the InSynC (SYP1-miniSOG-T2A-mCherry) construct

   Depositor

   Roger Tsien

   Article

   Lin et al Neuron. 2013 Jul 24;79(2):241-53. doi: 1

   Has Service

   AAV8

   Insert

   Synaptophysin-miniSOG-T2A-mCherry (Syp Rat)
   

   Use

   AAV

   Tags

   T2A-mCherry and miniSOG

   Expression

   Mutation

   Promoter

   human synapsin promoter

   Available Since

   Feb. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-tetO-Cdx2

    Plasmid

    #70697

    Purpose

    Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Cdx2

    Depositor

    Hubert Schorle

    Article

    Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

    Insert

    Cdx2 (Cdx2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetO

    Available Since

    Nov. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. (564) pAAV Alb-AAT KRAB-SadCas9 U6-mPcsk9

    Plasmid

    #163025

    Purpose

    Expression of CRISPRi with gRNA against mouse Pcsk9

    Depositor

    Tonia Rex

    Article

    Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

    Insert

    Mammalian codon-optimized SadCas9 (Pcsk9 S. aureus)
    

    Use

    AAV

    Tags

    KRAB, SV40 pA, and myc NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Alb-AAT

    Available Since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. EF1a_SOX2_P2A_Hygro_Barcode

    Plasmid

    #120480

    Purpose

    Barcoded lentiviral vector to express SOX2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    SOX2 (SOX2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGST2-GST-TEV-NDP52

    Plasmid

    #187828

    Purpose

    Plasmid for the expression and purification of GST-TEV-NDP52 in E. coli Rosetta pLysS. Internal reference: SMC1586

    Depositor

    Sascha Martens

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    NDP52 (CALCOCO2 Human)
    

    Use

    Tags

    GST-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAVS1-A2UCOE-EF1a-hCD46-P2A-HLA-G SCT-T2A-hCD47-E2A-hCD59-F2A-neo-AAVS1

    Plasmid

    #205443

    Purpose

    human AAVS1-targeting plasmid for expression of iCasp9, HLA-E SCT, human and CD55

    Depositor

    Deepta Bhattacharya

    Article

    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

    Insert

    hCD46-HLA-G SCT-hCD47-hCD59
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGFP-C3-Sec3

    Plasmid

    #53755

    Purpose

    pEGFP-C3 plasmid that expresses Sec3

    Depositor

    Channing Der

    Article

    Martin et al Mol Cell. 2014 Jan 23;53(2):209-20. doi:

    Insert

    Sec3 (EXOC1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pLT 653

    Plasmid

    #59996

    Purpose

    Expresses a hybrid dsRNA corresponding to the Caenorhabditis elegans dhc-3/klp-16 genes in bacteria; ingestion of the bacteria by C elegans produces an RNAi response & leads to more male progeny

    Depositor

    Lisa Timmons

    Article

    Timmons et al FEBS Lett. 2014 Jul 24. pii: S0014-5793(

    Inserts

    dhc-3 (dhc-3 Nematode)
    klp-16 (klp-16 Nematode)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    partial genomic

    Promoter

    Available Since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. Linked Free ClLIS1 (M)

    Plasmid

    #182487

    Purpose

    Yeast integrative plasmid for expressing fusion protein ERG20(F96W-N127W)-ClLIS1, with linker sequence AG4TGGA (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3)

    Depositor

    Claudia Vickers

    Article

    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

    Insert

    FPPS(M)-LS
    

    Use

    Synthetic Biology; Metabolic engineering

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL10

    Available Since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCSIIpuro-Necl5-ScNeo

    Plasmid

    #209901

    Purpose

    To monitor the status of Necl-5, the plasmid encodes a recombinant Necl-5 fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

    Depositor

    Michiyuki Matsuda

    Article

    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

    Insert

    Necl5-ScNeo (Pvr Mouse)
    

    Use

    Lentiviral

    Tags

    mNeonGreen and mScarlet

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPN089

    Plasmid

    #91614

    Purpose

    Express sgRNA targeting human FURIN

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    FURIN (FURIN Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    Oct. 12, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pmCTIP2-N174

    Plasmid

    #60858

    Purpose

    Expresses CTIP2 under the EF1a promoter

    Depositor

    Andrew Yoo

    Article

    Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1

    Insert

    CTIP2 isoform b (Bcl11b Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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