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We narrowed to 7,397 results for: ef1a;

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Showing: 7341 - 7360 of 7397 results

  7. EGFP-p300 (D1399Y)

    Plasmid
    #191763
    Purpose
    Expression of EGFP fused to catalytically dead core p300 histone acetyltransferase (D1399Y)
    Depositor
    Daniel Gerlich
    Article
    Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:
    Insert
    FRB-EGFP-p300 core (D1399Y) (EP300 Human)
    Tags
    EGFP (N-terminal of p300 core (D1399Y)) and FRB (…
    Expression
    Bacterial and Mammalian
    Mutation
    catalytic D1399Y mutation in p300
    Promoter
    EF1alpha
    Available Since
    Feb. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    191763-plasmid-map-sequence-id-383798

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  9. pCDH-EF1-FHC-POLQ-K121M

    Plasmid
    #64877
    Purpose
    lentiviral expression of human POLQ K121M mutant (a mutation in the conserved ATP-binding site of the Walker A motif in the helicase-like domain)
    Depositor
    Richard Wood
    Article
    Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.
    Insert
    POLQ (POLQ Human)
    Use
    Lentiviral
    Tags
    FLAG and HA
    Expression
    Mammalian
    Mutation
    K121M, a mutation in the conserved ATP-binding si…
    Promoter
    EF1alpha
    Available Since
    Oct. 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    64877-plasmid-map-sequence-id-163756

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  11. pJT055 AAVS1-BSD-9xTet0-pEF-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid
    #187324
    Purpose
    Donor to install a dual surface marker - fluorescence reporter into the AAVS1 safe harbor using HDR
    Depositor
    Michael Bassik, Lacra Bintu
    Article
    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038
    Insert
    BSD-9xTet0-pEF-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA
    Use
    CRISPR and TALEN
    Expression
    Mammalian
    Promoter
    pEF1a
    Available Since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    187324-plasmid-map-sequence-id-369800

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  14. L4385 dsRedExpress2 reporter, IL-10Rb NTEVp, IL-10Ra CTEVp in PiggyBac Transposon Vector

    Plasmid
    #244186
    Purpose
    PiggyBac transposon vector for expression of dsRedExpress2 under synTF promoter; constitutive expression of IL-10Rb NTEVp chain, IL-10Ra CTEVp chain, and mNeonGreen-P2A-PuroR selection marker
    Depositor
    Joshua Leonard
    Article
    Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158
    Insert
    dsRedExpress2 under synTF responsive promoter; IL-10Rb NTEVp chain with human CD8a SS; IL-10Ra CTEVp chain; mNeonGreen-P2A-PuroR (CD8A Human, Synthetic)
    Use
    Synthetic Biology
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    NTEVp_75S, CTEVp_190K
    Promoter
    hEF1a
    Available Since
    Oct. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    244186-plasmid-map-sequence-id-489485

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  15. L4342 TNFR1 CTEVp chain in PolyTX-mTagBFP2

    Plasmid
    #244178
    Purpose
    Constitutive expression of a MESA CTEVp chain encoding (N to C): TNFR1SS-3xFLAG-TNFR1ECD(dMMP)-TNFR1TMD-CTEVp(75S)-PRS(M)-VP64-ZF6
    Depositor
    Joshua Leonard
    Article
    Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158
    Insert
    MESA CTEVp chain with human TNFR1 SS, ECD (no MMP site) and TMD, CTEVp (190K), TEVp PRS (M in P1'), and synTF (VP64-ZF6) (TNFRSF1A Human, Synthetic)
    Use
    Synthetic Biology
    Tags
    TNFR1 signal peptide - 3xFLAG
    Expression
    Mammalian
    Mutation
    CTEVp_190K
    Promoter
    hEF1a
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    244178-plasmid-map-sequence-id-487910

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  16. L4341 TNFR1 NTEVp chain (deleted MMP site) in PolyTX-mNeonGreen

    Plasmid
    #244176
    Purpose
    Constitutive expression of a MESA NTEVp chain encoding (N to C): TNFR1SS-3xFLAG-TNFR1ECD(dMMP)-TNFR1TMD-NTEVp(75S)
    Depositor
    Joshua Leonard
    Article
    Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158
    Insert
    MESA NTEVp chain with human TNFR1 SS and ECD (no MMP site), murine CD28 TMD, and NTEVp 75S mutant (TNFRSF1A Human, Synthetic)
    Use
    Synthetic Biology
    Tags
    TNFR1 signal peptide - 3xFLAG
    Expression
    Mammalian
    Mutation
    NTEVp_75S
    Promoter
    hEF1a
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    244176-plasmid-map-sequence-id-487905

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  20. pJT340 Lenti pEF-dCas9-3XFLAG-KRAB[WSR7EEE] + MGA1+2 + RYBP-T2A-tagBFP-P2A-Blast

    Plasmid
    #187344
    Purpose
    dCas9 effector fusion for manipulating transcription
    Depositor
    Michael Bassik, Lacra Bintu
    Article
    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038
    Insert
    dCas9-3XFLAG-KRAB[WSR7EEE] + MGA1+2 + RYBP-T2A-tagBFP-P2A-Blast
    Use
    CRISPR and Lentiviral
    Tags
    3XFLAG
    Expression
    Mammalian
    Mutation
    KRAB WSR7EEE
    Promoter
    pEF1a
    Available Since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    187344-plasmid-map-sequence-id-373294

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Showing: 7341 - 7360 of 7397 results