19,660 results

1. pLenti-Cdc42-2G

   Plasmid

   #68813

   Purpose

   FRET-based biosensor reporting on endogenous Cdc42 activation (for Lentivirus production)

   Depositor

   Olivier Pertz

   Article

   Martin et al Sci Rep. 2016 Feb 25;6:21901. doi: 10.10

   Insert

   Cdc42 activation reporter
   

   Use

   Lentiviral

   Tags

   His and Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUCmini-iCAP-PHP.AX

   Plasmid

   #195218

   Purpose

   AAV capsid for broadly targeting cell types in the CNS (neurons including both excitatory and inhibitory neurons, astrocytes)

   Depositor

   Viviana Gradinaru

   Article

   Jang et al Nat Biotechnol. 2023 Jan 26. doi: 10.103

   Insert

   Synthetic construct isolate AAV-PHP.AX VP1 gene
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   p41

   Available Since

   March 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Fuw-dCas9-dead Tet1CD-P2A-BFP

   Plasmid

   #108246

   Purpose

   Lentiviral vector to express dCas9-dead Tet1CD-P2A-tagBFP

   Depositor

   Rudolf Jaenisch

   Article

   Liu et al Cell. 2018 Feb 8. pii: S0092-8674(18)300

   Insert

   dCas9-dead Tet1CD-HA-P2A-BFP
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   dCas9-D10A, dCas9-H840A, Tet1CD-H1672Y, Tet1CD-D1…

   Promoter

   Ubc

   Available Since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDZ Flag USP28

   Plasmid

   #15665

   Depositor

   Martin Eilers

   Article

   Popov et al Nat Cell Biol. 2007 Jul . 9(7):765-74.

   Insert

   USP28 (USP28 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Nov. 2, 2007

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. TALED_Left-ND1-AD-E1347A

   Plasmid

   #183894

   Purpose

   Expresses TALED_Left-ND1-AD-E1347A in mammalian cells

   Depositor

   Jin-Soo Kim

   Article

   Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

   Insert

   TALED for ND1 mutation
   

   Expression

   Mammalian

   Promoter

   pCMV

   Available Since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. mRFP-Ub

   Plasmid

   #11935

   Depositor

   Nico Dantuma

   Article

   Bergink et al Genes Dev. 2006 May 15. 20(10):1343-52.

   Insert

   Ubiquitin (RBX1 Human)
   

   Tags

   mRFP

   Expression

   Mammalian

   Available Since

   July 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3 MYC-Sumo3 WT

   Plasmid

   #48964

   Purpose

   Expresses MYC-Sumo3 Wild Type in mammalian cells

   Depositor

   Guy Salvesen

   Article

   Bekes et al J Biol Chem. 2011 Mar 25;286(12):10238-4

   Insert

   SUMO3 (SUMO3 Human)
   

   Tags

   MYC (EQKLISEED)

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   Nov. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pWZL Blast DN E-cadherin

   Plasmid

   #18800

   Depositor

   Bob Weinberg

   Article

   Onder et al Cancer Res. 2008 May 15. 68(10):3645-54.

   Insert

   Dom neg E-cadherin (Cdh1 Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   truncated form of E-cadherin (dominant negative) …

   Available Since

   July 15, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCR3.1-mIL2

   Plasmid

   #74467

   Purpose

   expression of mouse IL2 in mammalian cells

   Depositor

   Loree Heller

   Article

   Heller et al Melanoma Res. 2000 Dec;10(6):577-83.

   Insert

   IL2 (Il2 Mouse)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. EMTB-mCherry

    Plasmid

    #26742

    Depositor

    William Bement

    Article

    Miller et al Nat Cell Biol. 2009 Jan . 11(1):71-7.

    Insert

    ensconsin (MAP7 Human)
    

    Use

    Probe for microtubules

    Tags

    2x mCherry and Myc

    Expression

    Mammalian

    Mutation

    Microtubule binding-domain of ensconsin (amino ac…

    Available Since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX-2T PTP-1B

    Plasmid

    #8602

    Depositor

    Ben Neel

    Insert

    PTP-1B (PTPN1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. CD8a-EGFP

    Plasmid

    #86051

    Purpose

    A mammalian expression plasmid encoding CD8a with a C-terminus EGFP.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    CD8a (CD8A Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT7-EGFP-C1-HsDCP2

    Plasmid

    #25031

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

    Insert

    DCP2 (DCP2 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    June 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCFJ420 - Peft-3::GFP::H2B (ubiquitous)

    Plasmid

    #34877

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

    Insert

    Peft-3 GFP H2B tbb-2 UTR
    

    Use

    Worm targeting

    Mutation

    S65C

    Promoter

    eef-1A.1 (eft-3)

    Available Since

    Feb. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pQE80L-IL2 C125S

    Plasmid

    #104348

    Purpose

    Expresses human IL-2 (C125S)

    Depositor

    Alexander McLellan

    Insert

    Human IL-2 (C125S) (IL2 Human)
    

    Tags

    MRGSHHHHHGS-tag

    Expression

    Bacterial

    Mutation

    C125S mutation (TGT -> TCC)

    Promoter

    T5

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-FLAG-mTET2

    Plasmid

    #89735

    Purpose

    Expresses wildtype mouse TET2 in mammalian cells

    Depositor

    Yue Xiong

    Article

    Nakagawa et al Mol Cell. 2015 Jan 22;57(2):247-60. doi:

    Insert

    TET2 (Tet2 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRE118

    Plasmid

    #43830

    Depositor

    Dieter Schifferli

    Article

    Edwards et al Gene. 1998 Jan 30;207(2):149-57.

    Type

    Empty backbone

    Use

    Bacterial allelic exchange vector with sacb1

    Expression

    Bacterial

    Available Since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-Puro

    Plasmid

    #141386

    Purpose

    Lentiviral expression of SARS-CoV-2 membrane (M) protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    SARS-CoV-2-M (M SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available Since

    April 8, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. NPY2R-Tango

    Plasmid

    #66454

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    NPY2R (NPY2R Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pET21a-LysRS-His

    Plasmid

    #124114

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Lysine-tRNA ligase (lysU Escherichia coli)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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