We narrowed to 9,404 results for: pho;

1. pcDNA5 FRT TO myc Separase Non-Cleavable

   Plasmid

   #59826

   Purpose

   Allows the integration of myc Separase Non-Cleavable in the genome and Tet-inducible expression.

   Depositor

   Stephen Taylor

   Article

   Holland et al J Biol Chem. 2007 Aug 24;282(34):24623-3

   Insert

   Separase (ESPL1 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Non-Cleavable (E1483R, R1486E, E1503R, R1506E, E1…

   Promoter

   hybrid human cytomegalovirus (CMV)/TetO2 promoter

   Available Since

   Feb. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSUMO CPTP

   Plasmid

   #170735

   Purpose

   The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

   Depositor

   Rhoderick Brown

   Article

   Simanshu et al Nature. 2013 Aug 22;500(7463):463-7. doi

   Insert

   CPTP (CPTP Human)
   

   Tags

   SUMO

   Expression

   Bacterial

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1_GP1Fc Cl13

   Plasmid

   #138599

   Purpose

   Expresses GP1 of LCMV Cl13 fused with human IgG (Fc fusion)

   Depositor

   Annette Oxenius

   Article

   Krautler et al Cell Rep. 2020 Jan 28;30(4):997-1012.e6.

   Insert

   LCMV GP1-Fc
   

   Tags

   Fc fusion protein

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBabe-TetCMV-puro-mVenus-PA-Rac1-T17N

   Plasmid

   #22018

   Depositor

   Klaus Hahn

   Article

   Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

   Insert

   mVenus-PA-Rac1-T17N (RAC1 Human, Avena sativa (oat))
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   Rac1 starts at I4 and contains mutation T17N.

   Available Since

   Sept. 29, 2009

   Availability

   Academic Institutions and Nonprofits only

5. pTH726-CEN-RLuc/minCFLuc

   Plasmid

   #38210

   Depositor

   Tobias von der Haar

   Article

   Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

   Inserts

   Firefly Luciferase
   Renilla luciferase
   

   Expression

   Yeast

   Mutation

   All codons of the original FLuc gene were exchang…

   Promoter

   ADH1 and TDH3 (=GPD)

   Available Since

   Oct. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTIGER_tdTomato::3xFLAG::dCrk

   Plasmid

   #131138

   Purpose

   UASp construct for over-expression of Drosophila Crk with N-terminal tandem Tomato and 3X FLAG tag. Compatible with PhiC31-mediated site-specific integration or classical P-element insertion.

   Depositor

   Mark Peifer

   Article

   Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

   Insert

   Crk (Crk Fly)
   

   Tags

   3XFLAG and tdTomato

   Expression

   Insect

   Available Since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA6-N-3XFLAG-Fzd6

   Plasmid

   #123588

   Depositor

   Bruce Beutler

   Article

   Choi et al Science. 2019 May 10;364(6440). pii: 364

   Insert

   frizzled class receptor 6 (Fzd6 Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBabe-TetCMV-puro-mCerulean-PA-Rac1

   Plasmid

   #22031

   Depositor

   Klaus Hahn

   Article

   Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

   Insert

   mCerulean-PA-Rac1 (RAC1 Human, Avena sativa (oat))
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   Rac1 starts at I4 and contains mutations Q61L, E9…

   Available Since

   Sept. 29, 2009

   Availability

   Academic Institutions and Nonprofits only

9. pMXs-puro HA-Atg14*dCC

   Plasmid

   #38266

   Depositor

   Noboru Mizushima

   Article

   Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

   Insert

   autophagy related genes 14 (ATG14 Human)
   

   Use

   Retroviral

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   changed A618T, A621T, C624A and T627C for siRNA r…

   Promoter

   LTR

   Available Since

   Oct. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

10. GFP-CLCb (EED/QQN)

    Plasmid

    #47422

    Depositor

    Peter McPherson

    Article

    Poupon et al Proc Natl Acad Sci U S A. 2008 Jan 8;105

    Insert

    CLCb EED/QQN (CLTB Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    EED changed to QQN, in aa20-41 region

    Promoter

    CMV

    Available Since

    July 29, 2014

    Availability

    Academic Institutions and Nonprofits only

11. LENTICRISPR-HK2_sgRNA2

    Plasmid

    #201597

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Alexis Jourdain, Vamsi Mootha

    Article

    Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

    Insert

    HK2 (HK2 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA HA-Gnat1 WT-3UTR

    Plasmid

    #184026

    Purpose

    Expresses mouse Gnat1 protein with N-terminal HA tag from cDNA that contains the full length 3'-UTR

    Depositor

    Peter Stoilov

    Article

    Matalkah et al Commun Biol. 2022 Sep 24;5(1):1011. doi:

    Insert

    Gnat1 (Gnat1 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBabe 3xFLAG human Bmf

    Plasmid

    #17242

    Depositor

    Joan Brugge

    Article

    Schmelzle et al Proc Natl Acad Sci U S A. 2007 Mar 6. 10

    Insert

    Bmf (BMF Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    Jan. 18, 2008

    Availability

    Academic Institutions and Nonprofits only

14. pPICZ-mouseGIRK2R201A-eGFP-StII-6xHis

    Plasmid

    #124278

    Purpose

    Expresses mouse GIRK2 R201A as a TEV protease cleavable C-terminal fusion to eGFP, strep tag II, and 6x His tag

    Depositor

    Arthur Laganowsky

    Article

    Liu et al Nat Commun. 2019 Mar 22;10(1):1352. doi:

    Insert

    GIRK2 (Kcnj6 Mouse)
    

    Tags

    eGFP-StreptagII-6xHis

    Expression

    Yeast

    Mutation

    R201A

    Promoter

    AOX1

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSpCas9_BB_2A-GFP_MAPRE3-gRNA#2

    Plasmid

    #107730

    Purpose

    Knock out of EB3 in human cells by CRISPR/Cas9

    Depositor

    Torsten Wittmann

    Article

    van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

    Insert

    MAPRE3 gRNA #2 (targets Exon 1) (MAPRE3 Human)
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSpCas9_BB_2A-GFP_MAPRE3-gRNA#1

    Plasmid

    #107729

    Purpose

    Knock out of EB3 in human cells by CRISPR/Cas9

    Depositor

    Torsten Wittmann

    Article

    van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

    Insert

    MAPRE3 gRNA #1 (targets Exon 1) (MAPRE3 Human)
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLAP-BUBR1-dKARD

    Plasmid

    #114053

    Purpose

    transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

    Depositor

    Geert Kops

    Article

    Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

    Insert

    BUBR1-dKARD (BUB1B Human)
    

    Tags

    YFP-TEV-S- Human LAP

    Expression

    Mammalian

    Mutation

    C2823A and G2826A (siRNA-resistance): AGATACTAGCT…

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-SRGAP2A-FBAR-mRFP

    Plasmid

    #168502

    Purpose

    Expression of the F-BAR domain (aa1-501) of SRGAP2 with a C-terminal mRFP fusion.

    Depositor

    Franck Polleux

    Article

    Guerrier et al Cell. 2009 Sep 4;138(5):990-1004. doi: 1

    Insert

    SRGAP2A-FBAR (SRGAP2 Human)
    

    Tags

    mRFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP Kind2 S181E

    Plasmid

    #105312

    Purpose

    To study altered downstream functions of phospholylation-site mimetic kindlin2

    Depositor

    Kenneth Yamada

    Article

    Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

    Insert

    Kindlin2 (FERMT2 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S181E

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP Kind2 S181A

    Plasmid

    #105307

    Purpose

    To study altered downstream functions of phospholylation-site null kindlin2

    Depositor

    Kenneth Yamada

    Article

    Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

    Insert

    Kindlin2 (FERMT2 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S181A

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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