We narrowed to 23,290 results for: CRISPR

1. pCF204

   Plasmid

   #121656

   Purpose

   U6-sgRNA EFS-Cas9-wt-P2A-Puro

   Depositor

   David Savage

   Article

   Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

   Insert

   Cas9
   

   Use

   Lentiviral

   Promoter

   U6

   Available Since

   March 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_cpaA

   Plasmid

   #133344

   Purpose

   for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets cpaA gene's promoter from Caulobacter crescentus

   Depositor

   Michael Laub

   Article

   Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

   Insert

   sgRNA_cpaA
   

   Use

   CRISPR

   Promoter

   constitutive

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFETCh_ZGPAT

   Plasmid

   #86276

   Purpose

   Donor vector for 3' FLAG tag of human ZGPAT

   Depositor

   Eric Mendenhall, Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   ZGPAT homology arms (ZGPAT Human)
   

   Use

   CRISPR

   Tags

   3XFLAG-P2A-NeoR

   Available Since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pM117: VEGFA array presgRNA

   Plasmid

   #166869

   Purpose

   U6-driven expression of human VEGFA targeting array presgRNA compatible with CasRx.

   Depositor

   Guei-Sheung Liu

   Article

   Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

   Insert

   Human VEGFA targeting array presgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   July 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJK429

   Plasmid

   #155376

   Purpose

   PphlF_A4NT in dCRv1, 1x cascade + [dCas9 + PA4-mVenus]

   Depositor

   Pamela Silver

   Article

   Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

   Inserts

   dCas9 (bacteria)
   PA4-mVenus
   PphlF_A4NT in dCas9 sgRNA oscillator v1
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   D10A H840A (catalytically inactive)

   Available Since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKDsgRNA-malG

   Plasmid

   #89951

   Purpose

   Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting malG.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   malG gRNA
   

   Expression

   Bacterial

   Promoter

   pTet

   Available Since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFETCh_ZBTB7B

   Plasmid

   #135730

   Purpose

   Donor vector for 3' FLAG tag of human ZBTB7B

   Depositor

   Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   ZBTB7B Homology arms (ZBTB7B Human)
   

   Use

   CRISPR

   Tags

   3XFLAG-P2A-NeoR

   Available Since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. dSp-VP64-p65(150-261)-RTA(125-190)

   Plasmid

   #99674

   Purpose

   Expresses dSp Cas9 fused to combination of truncated activation domains

   Depositor

   George Church

   Article

   Vora et al bioRxiv 2018. 298620

   Insert

   dCas9
   

   Tags

   VP64-P65(151-261)-RTA(125-190)

   Expression

   Mammalian

   Mutation

   dead Cas9

   Available Since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKDsgRNA-hisG

   Plasmid

   #89954

   Purpose

   Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting hisG.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   hisG gRNA
   

   Expression

   Bacterial

   Promoter

   pTet

   Available Since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCF226

    Plasmid

    #121667

    Purpose

    EFS-Cas9-wt-P2A-Puro

    Depositor

    David Savage

    Article

    Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

    Insert

    cas9
    

    Use

    Lentiviral

    Promoter

    EF-1a core promoter

    Available Since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKDsgRNA-trmI

    Plasmid

    #89955

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting trmI.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    trmI gRNA
    

    Expression

    Bacterial

    Promoter

    pTet

    Available Since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pENTR-R4R3-stop

    Plasmid

    #113745

    Purpose

    Contains three stop codons in every frame to ensure a premature stop codon when introduced into the genome via homology-directed repair. Gateway multisite entry clone.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    stop cassette
    

    Use

    CRISPR; Gateway entry vector

    Mutation

    Added 3 stop codons to the original sequence; see…

    Available Since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28b-NmCas9-Flag H588A

    Plasmid

    #109032

    Purpose

    H588A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression

    Depositor

    Yan Zhang

    Article

    Rousseau et al Mol Cell. 2018 Mar 1;69(5):906-914.e4. d

    Insert

    CRISPR-associated endonuclease Cas9
    

    Tags

    Flag Tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFETCh_SOX5

    Plasmid

    #86292

    Purpose

    Donor vector for 3' FLAG tag of human SOX5

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    SOX5 homology arms (SOX5 Human)
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Available Since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFETCh_ZNF644

    Plasmid

    #86264

    Purpose

    Donor vector for 3' FLAG tag of human ZNF644

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    ZNF644 homology arms (ZNF644 Human)
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Available Since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKDsgRNA-xylA

    Plasmid

    #89964

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting xylA.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    xylA gRNA
    

    Expression

    Bacterial

    Promoter

    pTet

    Available Since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKDsgRNA-lpoB

    Plasmid

    #89959

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting lpoB.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    lpoB gRNA
    

    Expression

    Bacterial

    Promoter

    pTet

    Available Since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pU6_(GLuc)_INT[GFPApt]

    Plasmid

    #68428

    Purpose

    Transient expression in mammalian cells of an "INT" construct_bearing the GFP aptamer, targeting the GLuc reporter. U6 promoter

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    INT construct_bearing the GFP aptamer
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    human U6

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pU6_(GLuc)_INT[3xK-T]

    Plasmid

    #68429

    Purpose

    Transient expression in mammalian cells of an "INT" construct_bearing three kink-turns, targeting the GLuc reporter. U6 promoter

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    INT construct_bearing three kink-turns
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    human U6

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKO025

    Plasmid

    #242913

    Purpose

    aTc-inducible expression of Spy-dCas9-AsiA and Sth1-dCas9, and constitutive expression of MCP-SoxS

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

    Insert

    Sth1-dCas9
    

    Use

    CRISPR and Synthetic Biology

    Promoter

    Ptet

    Available Since

    Oct. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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