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pMGS56 (GFP-ARF16-PB1-P2A-OsTIR1)

Plasmid

#129668

Purpose

A repair construct to express GFP-ARF16-PB1 and OsTIR1 under the control of the CMV promoter from the human AAVS1 locus

Depositor

Michael Guertin

Article

Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

Insert

OsARF16-PB1 domain

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pSH-EFIRES-P-AtAFB2-mCherry

Plasmid

#129716

Purpose

AAVS1 safe harbor targeting vector expressing AtAFB2-mCherry

Depositor

Elina Ikonen

Article

Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

Insert

AFB2 (AFB2 Mustard Weed)

Use

CRISPR and TALEN; Human safe harbor locus (a avs1…

Tags

mCherry

Expression

Mammalian

Mutation

codon optimized

Promoter

EF1a

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pMGS55 (GFP-ARF16-PB1-MCS-P2A-OsTIR1)

Plasmid

#129667

Purpose

A repair construct to express GFP-ARF16-PB1-MCS and OsTIR1 under the control of the CMV promoter from the human AAVS1 locus

Depositor

Michael Guertin

Article

Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

Insert

OsARF16-PB1 domain

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
mGDF8 donor CFP-hDF

Plasmid

#72590

Purpose

Plasmid contains left and right homology arms for mouse GDF8 flanking the mCherry-puromycin-2A-ECFP-dysferlin cassette.

Depositor

Renzhi Han

Article

Xu et al Mol Ther Nucleic Acids. 2013 Jul 30;2:e1

Insert

dysferlin (DYSF Human)

Use

Tags

ECFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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hGDF8 donor CFP-hDF

Plasmid

#72591

Purpose

Plasmid contains left and right homology arms for human GDF8 flanking the mCherry-puromycin-2A-ECFP-dysferlin cassette.

Depositor

Renzhi Han

Article

Xu et al Mol Ther Nucleic Acids. 2013 Jul 30;2:e1

Insert

dysferlin (DYSF Human)

Use

Tags

ECFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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pSH-EFIRES-P-Seipin-miniIAA7-mScarlet-I

Plasmid

#129721

Purpose

AAVS1 safe harbor targeting vector expressing miniIAA7-mScarlet-I tagged Seipin

Depositor

Elina Ikonen

Article

Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

Insert

Seipin (BSCL2 Human)

Use

CRISPR and TALEN; Human safe harbor locus (a avs1…

Tags

miniIAA7-mScarlet-I

Expression

Mammalian

Mutation

silent mutations

Promoter

EF1a

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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Hygro-Cas9 donor

Plasmid

#86883

Purpose

Donor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locus with hygromycin selection

Depositor

Danwei Huangfu

Article

Shi et al Cell Stem Cell. 2017 Feb 8. pii: S1934-5

Insert

hSpCas9

Use

CRISPR

Tags

3xFlag, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Mutation

Promoter

Available since

March 2, 2017

Availability

Academic Institutions and Nonprofits only

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Cre-p2a-tdTomato

Plasmid

#176837

Purpose

Express Cre in mammalian cells and enable FACS sorting Cre expressing cells by tdTomato expression

Depositor

Rudolf Jaenisch

Article

Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

Insert

Cre-P2A-tdTomato

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAGGS

Available since

Nov. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pSH-EFIRES-B-Seipin-miniIAA7-mEGFP

Plasmid

#129719

Purpose

AAVS1 safe harbor targeting vector expressing miniIAA7-mEGFP tagged Seipin

Depositor

Elina Ikonen

Article

Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

Insert

Seipin (BSCL2 Human)

Use

CRISPR and TALEN; Human safe harbor locus (a avs1…

Tags

miniIAA7-mEGFP

Expression

Mammalian

Mutation

silent mutations

Promoter

EF1a

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pSH-EFIRES-P-AtAFB2-mCherry-weak NLS

Plasmid

#129717

Purpose

AAVS1 safe harbor targeting vector expressing AtAFB2-mCherry-weak NLS

Depositor

Elina Ikonen

Article

Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

Insert

AFB2 (AFB2 Mustard Weed)

Use

CRISPR and TALEN; Human safe harbor locus (a avs1…

Tags

mCherry-weak NLS

Expression

Mammalian

Mutation

codon optimized

Promoter

EF1a

Available since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge