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Drosophila Usp46-V5

Plasmid

#217620

Purpose

Mammalian expression of V5 tagged Drosophila Usp46

Depositor

Ethan Lee

Article

Spencer et al Nat Commun. 2023 Oct 5;14(1):6174. doi:

Insert

USP46 (Usp12-46 Fly)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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p667-UBC-MAFG-V5-miniTurbo

Plasmid

#207818

Purpose

C-terminal tagged MAFG for miniTurbo proximity experiments

Depositor

Ben Major

Article

LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

Insert

MAFG-V5-miniTurbo

Use

Lentiviral

Tags

V5-miniTurbo

Expression

Mutation

Promoter

UBC

Available since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-MAFG

Plasmid

#207817

Purpose

N-terminal tagged MAFG for miniTurbo proximity experiments

Depositor

Ben Major

Article

LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

Insert

miniTurbo-V5-MAFG

Use

Lentiviral

Tags

miniTurbo-V5

Expression

Mutation

Promoter

UBC

Available since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-ZNF746

Plasmid

#207819

Purpose

N-terminal tagged ZNF746 for miniTurbo proximity experiments

Depositor

Ben Major

Article

LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

Insert

miniTurbo-V5-ZNF746 (ZNF746 Human)

Use

Lentiviral

Tags

miniTurbo-V5

Expression

Mutation

Promoter

UBC

Available since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-Cas9-NS-A

Plasmid

#207823

Purpose

Dual expression of Cas9 and non-specific sgRNA

Depositor

Ben Major

Article

LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

Insert

non-specific sgRNA

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mutation

Promoter

EF-1a; U6

Available since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-Cas9-NS-B

Plasmid

#207824

Purpose

Dual expression of Cas9 and non-specific sgRNA

Depositor

Ben Major

Article

LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

Insert

non-specific sgRNA

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mutation

Promoter

EF-1a; U6

Available since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCW57.1-ZNF746-C571A

Plasmid

#207826

Purpose

Dox-inducible expression of ZNF746-C571A

Depositor

Ben Major

Article

LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

Insert

ZNF746-C571A (ZNF746 Human)

Use

Lentiviral

Tags

Expression

Mutation

C571A

Promoter

TRE

Available since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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p667-UBC-ZNF746-V5-miniTurbo

Plasmid

#207820

Purpose

C-terminal tagged ZNF746 for miniTurbo proximity experiments

Depositor

Ben Major

Article

LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

Insert

ZNF746-V5-miniTurbo (ZNF746 Human)

Use

Lentiviral

Tags

V5-miniTurbo

Expression

Mutation

Promoter

UBC

Available since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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hARL15

Plasmid

#209798

Purpose

Bacterial expression of human ARL15

Depositor

Kalle Gehring

Article

Mahbub et al Elife. 2023 Jul 14;12:e86129. doi: 10.75

Insert

ARL15 (ARL15 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pQTEV-C6orf60

Plasmid

#34813

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

Insert

C6orf60 (FAM184A Human)

Use

Tags

His and TEV

Expression

Bacterial

Mutation

Promoter

Available since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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pLenti-TripleColour-StAR

Plasmid

#222695

Purpose

Competition assay

Depositor

Ulrich Elling

Article

Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

TagBFP, miRFP703, dTomato

Expression

Mammalian

Mutation

PGK, TagBFP, dTomato without BsmBI cutsite

Promoter

Available since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-pAce-Kv2.1PR

Plasmid

#195529

Purpose

Green fluorescent, positive response-polarity voltage indicator under the control of CaMKII promoter; soma-targeted

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

pAce-Kv2.1 proximal restriction sequence

Use

AAV

Tags

Expression

Mammalian

Mutation

Ace-mNeon 78K, 81D, 92N, 178F; SY linker

Promoter

CaMKII

Available since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pKL123

Plasmid

#187889

Purpose

AAVS1 safe-harbor integration of dox-inducible NFIA-SOX9

Depositor

Martin Kampmann

Article

Leng et al Nat Neurosci. 2022 Nov;25(11):1528-1542.

Insert

NFIA-SOX9

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-fDIO-pAce-Kv2.1PR

Plasmid

#195535

Purpose

Flp recombinase-dependent green fluorescent, positive response-polarity voltage indicator; soma-targeted

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

pAce-Kv2.1 proximal restriction sequence

Use

AAV

Tags

Expression

Mammalian

Mutation

Ace-mNeon 78K, 81D, 92N, 178F; SY linker

Promoter

CAG

Available since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO-Ace-mNeon2-Kv2.1PR

Plasmid

#195532

Purpose

Cre-dependent expression of a green fluorescent, negative response-polarity voltage indicator; soma-targeted

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

Ace-mNeon2-Kv2.1 proximal restriction sequence

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Ace-mNeon 81S; SY linker

Promoter

CAG

Available since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO-pAceR-Kv2.1PR

Plasmid

#195534

Purpose

Cre-dependent red fluorescent, positive response-polarity voltage indicator; soma-targeted

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

pAceR-Kv2.1 proximal restriction sequence

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

VARNAM 78E, 81D, 92N; SI linker

Promoter

CAG

Available since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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RAD21 C-tag CRISPR

Plasmid

#140540

Purpose

RAD21 tagging CRISPR

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

RAD21 (RAD21 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO-VARNAM2-Kv2.1PR

Plasmid

#195533

Purpose

Cre-dependent expression of a red fluorescent, negative response-polarity voltage indicator; soma-targeted

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

VARNAM2-Kv2.1 proximal restriction sequence

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

VARNAM SI linker

Promoter

CAG

Available since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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p667-UBC-NRF1-V5-miniTurbo

Plasmid

#207810

Purpose

C-terminal tagged NRF1 for miniTurbo proximity experiments

Depositor

Ben Major

Article

LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

Insert

NRF1-V5-miniTurbo (NFE2L1 Human)

Use

Lentiviral

Tags

V5-miniTurbo

Expression

Mutation

Promoter

UBC

Available since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-NRF1

Plasmid

#207809

Purpose

N-terminal tagged NRF1 for miniTurbo proximity experiments

Depositor

Ben Major

Article

LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

Insert

miniTurbo-V5-NRF1 (NFE2L1 Human)

Use

Lentiviral

Tags

miniTurbo-V5

Expression

Mutation

Promoter

UBC

Available since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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