We narrowed to 9,829 results for: pho;

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pcDNA3 PARL-FLAG-CT S65D+T69D+S70D

Plasmid

#13617

Depositor

Luca Pellegrini

Article

Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

Insert

Presenilin-associated rhomboid-like (PARL, PARL Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine 65, Thr 69 and Ser70 to Asp

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pGEM-PH-citrine

Plasmid

#131406

Purpose

Purpose: synthesis of mRNA encoding a membrane marker. Insert: PH domain of the human phospholipase C delta 1 (PLCD1) fused with the yellow fluorescent protein mCitrine

Depositor

Lars Hufnagel

Article

Guignard et al Science. 2020 Jul 10;369(6500). pii: 369

Insert

PH

Use

In vitro transcription

Tags

citrine yellow fluorescent protein

Promoter

T7

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pRSETa-mEos4b-V69T

Plasmid

#98572

Purpose

photoconvertible fluorescent protein mEos4b-V69T in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression

Depositor

Ulrike Endesfelder

Article

Turkowyd et al Angew Chem Int Ed Engl. 2017 Jun 2. doi:

Insert

mEos4b-V69T

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

March 9, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3 PARL-FLAG-CT S65A+T69A+S70A

Plasmid

#13616

Depositor

Luca Pellegrini

Article

Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

Insert

Presenilin-associated rhomboid-like (PARL, PARL Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed Ser 65, Thr 69 and Ser 70 to Alanine

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pHJ425

Plasmid

#20683

Depositor

Stephen Shaw

Article

Hao et al J Cell Biol. 2009 Feb 9. 184(3):451-62.

Insert

Ezrin (EZR Human)

Tags

GFP

Expression

Mammalian

Mutation

Changed Lys 253, 254, 262, 263 to Asn; and change…

Available Since

April 14, 2009

Availability

Academic Institutions and Nonprofits only
pETM6_Ptrc_BsMdh_BmHps_BmPhi_Pthl_CaThl_CaCtfAB_CaAdc

Plasmid

#129105

Purpose

Expresses Mdh from Bacillus subtilis and Hps & Phi from Bacillus methanolicus as well as Clostridium acetobutylicum genes required for Acetate-dependent Acetone production

Depositor

Eleftherios Terry Papoutsakis

Article

Bennett et al Metab Eng. 2018 Jan;45:75-85. doi: 10.10

Insert

BsMDH BmHPS BmPHI CaTHL CaCtfAB CaADC

Expression

Bacterial

Available Since

Nov. 11, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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MyoUTipGAPi

Plasmid

#127549

Purpose

Positive RNAi control for pGAPi-based RNAi silencing in tandem with APT transcript segment

Depositor

Luis Vidali

Article

Orr et al Plant Physiol. 2020 Aug 6. pii: pp.20.00

Insert

Myosin XIa+b 5'UTR and Adenine Phosphorybosyltransferase transcript segment

Use

RNAi; Positive control

Promoter

Ubiquitin

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-ERK5 713

Plasmid

#65246

Purpose

Expression of HA tagged ERK5 AA 2-713 in mammalian cells

Depositor

Axel Ullrich

Article

Buschbeck et al J Biol Chem. 2002 Aug 16;277(33):29503-9

Insert

ERK5 713 (MAPK7 Human)

Tags

HA

Expression

Mammalian

Mutation

deleted AA 714-817

Promoter

CMV

Available Since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only
pENTR2b-PTPN12(D199A,C231S)

Plasmid

#215516

Purpose

Gateway entry vector with a phosphatase-inactive PTP-PEST mutant (D199A,C231S)

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

PTPN12 (PTPN12 Human)

Use

Gateway Cloning

Mutation

D199A, C231S (Phosphatase inactive)

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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PDE4B-Sensor

Plasmid

#208929

Purpose

in vitro JNK-Sensor for luminescent detection of JNK-activity

Depositor

Attila Reményi

Article

Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854.

Insert

PDE4B docking sequence+Phospho site and Smbit (PDE4B Human)

Tags

HISx6, MBP, and Smbit

Expression

Bacterial

Promoter

T7

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR4 genomic A. thaliana PLDGAMMA3

Plasmid

#209188

Purpose

pENTR4 plasmid with genomic sequence of Arabidopsis thaliana PHOSPHOLIPASE D GAMMA 3 (PLDGAMMA3, AT4G11840) without stop codon for C-terminal epitope tags

Depositor

Lennart Wirthmueller

Article

Simranjit et al bioRxiv 2023.09.25.558804

Insert

AT4G11840 (PLDGAMMA3 Mustard Weed)

Use

Gateway Cloning

Promoter

none

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pMx/ATP10A(E203Q)-HA

Plasmid

#209258

Purpose

Mammalian expression of ATP10A mutant E203Q

Depositor

Hye-Won Shin

Article

Naito et al J Biol Chem. 2015 Jun 12;290(24):15004-1

Insert

ATP10A (ATP10A Human)

Use

Retroviral

Tags

HA

Mutation

E203Q

Available Since

Nov. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG/ATP10A(E203Q)-HA

Plasmid

#209246

Purpose

Mammalian expression of ATP10A mutant E203Q

Depositor

Hye-Won Shin

Article

Naito et al J Biol Chem. 2015 Jun 12;290(24):15004-1

Insert

ATP10A (ATP10A Human)

Tags

HA

Expression

Mammalian

Mutation

E203Q

Available Since

Nov. 3, 2023

Availability

Academic Institutions and Nonprofits only

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hHSF4b K294R

Plasmid

#118357

Purpose

This plasmid expresses human HSF4b K294R mutant, which cannot undergo phosphorylation-dependent sumoylation.

Depositor

Lea Sistonen

Article

Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

Insert

hHSF4b (HSF4 Human)

Expression

Mammalian

Mutation

K294R

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pBabe puro HA PIK3CA H1047R

Plasmid

#12524

Purpose

Retroviral expression of HA tagged phosphoinositide 3-kinase H1047R (Tumor-derived allele)

Depositor

Jean Zhao

Article

Zhao et al Proc Natl Acad Sci U S A. 2005 Dec 20. 1

Insert

PIK3CA H1047R (PIK3CA Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

H1047R. Tumor-derived allele.

Available Since

Aug. 18, 2006

Availability

Academic Institutions and Nonprofits only

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GFP-PH-FAPP1

Plasmid

#161986

Purpose

Expresses GFP-tagged PH-FAPP1. PI(4)P biosensor

Depositor

Tamas Balla, David Bryant

Article

Balla et al Mol Biol Cell. 2005 Mar;16(3):1282-95. d

Insert

PH-FAPP1 (PLEKHA3 Human)

Tags

GFP

Expression

Mammalian

Available Since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pKT-CNP

Plasmid

#211803

Purpose

Expresses 2',3'-cyclic nucleotide phosphodiesterase catalytic domain

Depositor

Emily Weinert

Article

Fontaine et al Biochem J. 2018 Apr 30;475(8):1491-1506.

Insert

2',3'-cyclic nucleotide phosphodiesterase catalytic domain (Cnp Rat)

Expression

Bacterial

Promoter

tet

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pET15b+His SUMO PPM1H

Plasmid

#207333

Purpose

Bacterial expression of His SUMO PPM1H

Depositor

Suzanne Pfeffer

Article

Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

PPM1H (PPM1H Human)

Tags

His-SUMO

Expression

Bacterial

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pLL3.7m-psMEK2

Plasmid

#89363

Purpose

A lenti-viral vector expressing photoswitchable MEK2 in mammalian cells

Depositor

Michael Lin

Article

Zhou et al Science. 2017 Feb 24;355(6327):836-842.

Insert

psMEK2 (MAP2K2 Synthetic, Human)

Use

Lentiviral

Tags

Human influenza hemagglutinin (HA) tag and pdDron…

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pShuttle-mRFP-gsk3 s9a

Plasmid

#24371

Depositor

Torsten Wittmann

Article

Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

Insert

GSK3beta(S9A) (GSK3B Human)

Use

Adenoviral

Tags

mRFP

Expression

Mammalian

Mutation

S9A. Mutant version of GSK3 that lacks the inhibi…

Available Since

May 13, 2010

Availability

Academic Institutions and Nonprofits only

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