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ldlr (low density lipoprotein receptor)_R (OZ540)

Plasmid

#27217

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting ldlr (LOC100329978 Zebrafish)

Use

Zebrafish targeting

Tags

Expression

Mutation

Promoter

Available since

Feb. 8, 2011

Availability

Academic Institutions and Nonprofits only
ldlr (low density lipoprotein receptor)_L (OZ539)

Plasmid

#27216

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting ldlr (LOC100329978 Zebrafish)

Use

Zebrafish targeting

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only
pDEST26 Ago2 S387E

Plasmid

#59906

Purpose

Expresses 6xHis-Ago2 (N-termi tag) S387E mutant in mammalian cells

Depositor

Carl Novina

Article

Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

Insert

Argonaute 2 (AGO2 Human)

Use

Tags

6xHis (N-termi tag)

Expression

Mammalian

Mutation

S387E, phospho-dominant

Promoter

CMV

Available since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

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zgc:65890_L (OZ599)

Plasmid

#35239

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting zgc:65890 (zgc:65890 Zebrafish)

Use

Zebrafish targeting

Tags

Expression

Mutation

Promoter

Available since

March 16, 2012

Availability

Academic Institutions and Nonprofits only
zgc:65890_R (OZ600)

Plasmid

#35240

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting zgc:65890 (zgc:65890 Zebrafish)

Use

Zebrafish targeting

Tags

Expression

Mutation

Promoter

Available since

March 5, 2012

Availability

Academic Institutions and Nonprofits only
VDAC2_R (OZ524)

Plasmid

#27199

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting VDAC2 (vdac2 Zebrafish)

Use

Zebrafish targeting

Tags

Expression

Mutation

Promoter

Available since

Feb. 8, 2011

Availability

Academic Institutions and Nonprofits only
REST corepressor 2_L (OZ553)

Plasmid

#28078

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting REST corepressor 2 (rcor2 Zebrafish)

Use

Zebrafish targeting

Tags

Expression

Mutation

Promoter

Available since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only
REST corepressor 2_R (OZ554)

Plasmid

#28079

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting REST corepressor 2 (rcor2 Zebrafish)

Use

Zebrafish targeting

Tags

Expression

Mutation

Promoter

Available since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only
pBIG1e-CDK12-KiD/Cyclin K

Plasmid

#231743

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Martinez et al Molecular Cell. 01 Mar 2025. doi: 10.101

Inserts

CDK12 (CDK12 Human)
CCNK (CCNK Human)

Use

Tags

Flag-3C

Expression

Insect

Mutation

CDK12(700-1082)

Promoter

Available since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-[BsmBI_pegRNA_entry]-tevopreQ1-term (LM1138)

Plasmid

#223137

Purpose

pU6 entry plamid for cloning prime editor tevopreQ1 epegRNAs (requires BsmBI or Esp3I digest and ligation of duplexed oligos)

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

etevopreQ1 entry plasmid backbone for epegRNA expression via human U6 promoter

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-SpCas9_gRNA(RHO-P23H)-CMV-mTagBFP2_(RAS3613)

Plasmid

#228252

Purpose

pU6 (human U6) expression of SpCas9 sgRNA targeting RHO P23H mutation and pCMV expression of mTagBFP2

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

SpCas9 P23H sgRNA, mTagBFP2

Use

Tags

Expression

Mammalian

Mutation

n/a

Promoter

CMV and U6

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-BsmBI_epegRNA_entry-mpknot_(LM1140)

Plasmid

#228253

Purpose

pU6 entry plamid for cloning prime editor mpknot epegRNAs (requires BsmBI or Esp3I digest and ligation of duplexed oligos)

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

mpknot entry plasmid backbone for epegRNA expression via human U6 promoter

Use

Tags

Expression

Mammalian

Mutation

n/a

Promoter

U6

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-U6/TO-shEsr1-CMV-TetR-eGFP

Plasmid

#233298

Purpose

DOX-inducible U6 driven shRNA against mouse Esr1

Depositor

Dayu Lin

Article

Golden et al bioRxiv [Preprint]. 2024 Sep 25:2023.12.

Insert

Esr1 shRNA (Esr1 Mouse)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-CRISPRv2-sgIl1r1_#2-hygro

Plasmid

#231990

Purpose

Knockout mouse Il1r1

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

sgRNA with Cas9 and hygromycin resistance (Il1r1 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-multi-CRISPR-sgRipk3_#2-puro

Plasmid

#231982

Purpose

Knockout mouse Ripk3

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

sgRNA with Cas9 with puromycin resistance (Ripk3 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-multi-CRISPR-sgRipk3_#1-puro

Plasmid

#231981

Purpose

Knockout mouse Ripk3

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

sgRNA with Cas9 with puromycin resistance (Ripk3 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-multi-CRISPR-sgMlkl_#2-puro

Plasmid

#231980

Purpose

Knockout mouse Mlkl

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

sgRNA with Cas9 with puromycin resistance (Mlkl Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-multi-CRISPR-sgMlkl_#1-puro

Plasmid

#231979

Purpose

Knockout mouse Mlkl

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

sgRNA with Cas9 with puromycin resistance (Mlkl Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-multi-CRISPR-sgCasp8_#2-puro

Plasmid

#231978

Purpose

Knockout mouse Casp8

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

sgRNA with Cas9 with puromycin resistance (Casp8 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-multi-CRISPR-sgCasp8_#1-puro

Plasmid

#231977

Purpose

Knockout mouse Casp8

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

sgRNA with Cas9 with puromycin resistance (Casp8 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge