We narrowed to 15,732 results for: LEA

1. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-B103 (LM2963)

   Plasmid

   #208960

   Purpose

   A variant CE1 construct with B103 DNA polymerase, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   PCV2-XTEN-nSpCas9-BPNLS-B103-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A)

   Promoter

   CMV and T7

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-PolBeta (JO574)

   Plasmid

   #208953

   Purpose

   A variant CE1 construct with human DNA polymerase beta, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   PCV2-XTEN-nSpCas9-BPNLS-PolBeta-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A)

   Promoter

   CMV and T7

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHAGE2-TetOminiCMV-OS

   Plasmid

   #136576

   Purpose

   Dox-inducible polycistronic lentiviral vector expressing mouse Oct4, Sox2

   Depositor

   Hans Schöler

   Article

   Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

   Insert

   Oct4-P2A-Sox2 (Pou5f1, Sox2 Mouse)
   

   Use

   Lentiviral

   Expression

   Bacterial

   Promoter

   tetO-miniCMV (dox-inducible)

   Available Since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. mGLDC

   Plasmid

   #72881

   Purpose

   overexpression

   Depositor

   David Sabatini

   Article

   Kim et al Nature. 2015 Apr 16;520(7547):363-7. doi

   Insert

   Gldc (Gldc Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA-GFP-EphrinB3-Flag(A227Y)

   Plasmid

   #155013

   Purpose

   Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EphrinB3 A227Y variant from pcDNA3.1

   Depositor

   Sinisa Urban

   Article

   Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

   Insert

   EphrinB3 (EPHB3 Human)
   

   Tags

   Flag, GFP, and signal peptide (residues 1 to 32) …

   Expression

   Mammalian

   Mutation

   A227Y

   Promoter

   CMV

   Available Since

   Nov. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. TV-hPITX3-Reverse

   Plasmid

   #22208

   Depositor

   Rudolf Jaenisch

   Article

   Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

   Insert

   PITX3-eGFP-loxP-PGK-Puro-loxP (PITX3, PGK1 Human)
   

   Use

   Zfn donor

   Available Since

   Oct. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pROF346

   Plasmid

   #155331

   Purpose

   Binary vector for expression of PULSE system (under the control of AtUbi10 promoter) controlling Venus-H2B. It constitutively expresses Cerulean-NLS and a plant selection cassette.

   Depositor

   Matias Zurbriggen

   Article

   Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

   Insert

   RB_KanR_Tnos-NLS-PIF6-E-PUbi10_Tnos-nCerulean-PUbi10_Tnos-NLS-VP16-PhyB-PUbi10_Tnos-EL222-NLS-SRDX-PUbi10_T35S-nVenus-POpto_LB
   

   Use

   Synthetic Biology

   Expression

   Plant

   Available Since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCEP4-BG505.SOSIP-QES.i03.c01-TM

   Plasmid

   #111845

   Purpose

   Mammalian expression plasmid for BG505 SOSIP.664 fused to a transmembrane tether; QES mutant with enhanced binding to PG16

   Depositor

   Erik Procko

   Article

   Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

   Insert

   HIV-1 Env (BG505 SOSIP.664)
   

   Tags

   CD5 leader sequence and TM helix from MHC class I

   Expression

   Mammalian

   Mutation

   Codon-optimized synthetic gene; has SOSIP mutatio…

   Promoter

   CMV

   Available Since

   March 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-EFS-DIO-GRAB_CRFmut

   Plasmid

   #208663

   Purpose

   Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) control sensor GRAB_CRFmut in a cre-dependent manner

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   GPCR activation based corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut
   

   Use

   AAV

   Promoter

   EFS

   Available Since

   Oct. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCDNA3.1-Kozak-SP-SNAPf-lk-EK-ADGRL3-CTF

    Plasmid

    #201217

    Purpose

    pCDNA3.1 with Kozak (GCCACC), ADGRL3 signal peptide, SNAP-tag, GS linker, and Flag-tag (EK site) ahead of ADGRL3-CTF

    Depositor

    Jonathan Javitch

    Article

    Perry-Hauser et al J Biol Chem. 2022 Dec;298(12):102594. do

    Insert

    ADGRL3 (Adgrl3 Mouse)
    

    Tags

    Flag and SNAP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCAGGS-SARS2-S-FKO (C-flag)

    Plasmid

    #159364

    Purpose

    Mammalian expression of SARS-CoV-2 S protein (FKO: furin-cleavage site mutant) with the flag tag at C-terminus.

    Depositor

    Hyeryun Choe, Michael Farzan

    Article

    Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

    Insert

    SARS-CoV-2 S protein (S SARS-CoV-2)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Furin-cleavage site mutation

    Promoter

    CMV

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBactin-mOrange2-NEDD1-gTBD

    Plasmid

    #196858

    Purpose

    Expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to mOrange. Used to displace endogenous gamma-TuRC from the centrosome.

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    mOrange2-N-gTBD (NEDD1 Human)
    

    Expression

    Mammalian

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available Since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCAGGS-SMS1-TEV-Twin-Strep

    Plasmid

    #202528

    Purpose

    Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human Sphingomyelin synthase 1 (SMS1) in mammalian cell

    Depositor

    Chiaki Murakami

    Article

    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

    Insert

    SGMS1 (SGMS1 Human)
    

    Tags

    TEV cleavable site and Twin-Strep-tag

    Expression

    Mammalian

    Mutation

    deleted stop codon (TAA)

    Promoter

    CAG and chicken β-actin promoter

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Control click editor - pCMV-T7-PCV2-deadCas9-EcKlenow (EMK500)

    Plasmid

    #208944

    Purpose

    A control CE1 construct with catalytically inactivated SpCas9, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-dSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    dSpCas9(D10A/H840A);EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available Since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. TVBB C-term-mTurquoise2-Puro

    Plasmid

    #169224

    Purpose

    Targeting vector backbone to support a knock-in of Linker-mTurquoise2-P2A-Puro at the C-terminus of a target locus

    Depositor

    Hugo Snippert

    Article

    Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

    Insert

    Double SapI flanked mTurquoise2-P2A-Puro
    

    Use

    Targeting vector backbone

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV-hAR-CA576-Z

    Plasmid

    #89108

    Purpose

    mammalian expression of full-length human androgen receptor with DNA binding domain mutation CA576

    Depositor

    Elizabeth Wilson

    Article

    Zhou et al J Biol Chem. 1994 May 6;269(18):13115-23

    Insert

    hAR-C576A (AR Human)
    

    Expression

    Mammalian

    Mutation

    C576A

    Promoter

    CMV

    Available Since

    April 21, 2017

    Availability

    Academic Institutions and Nonprofits only

17. pBactin-EGFP-NEDD1-gTBD

    Plasmid

    #196857

    Purpose

    Expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to enhanced (E) GFP. Used to displace endogenous gamma-TuRC from the centrosome.

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    EGFP-N-gTBD (NEDD1 Human)
    

    Expression

    Mammalian

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGST-HsSyx-375-792

    Plasmid

    #179292

    Purpose

    E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 375-792) with N-terminal His6 + GST + TEV protease cleavage site

    Depositor

    Debra Hansen

    Article

    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.

    Insert

    Syx
    

    Tags

    His6 + GST + TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    residues 375-792 from accession number NP_0010361…

    Promoter

    T7

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHS0535 CRISPR-associated IscB (Chesapeake Bay) in pBR322 with Fn spacer

    Plasmid

    #176589

    Purpose

    Endogenous locus of CRISPR-associated IscB (Chesapeake Bay) with Fn spacer

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

    Insert

    CRISPR-associate IscB (Chesapeake Bay) locus
    

    Expression

    Bacterial

    Mutation

    Truncated CRISPR array to two direct repeats, rep…

    Available Since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HA-GFP1-10-actin-pcDNA4-TO

    Plasmid

    #118370

    Purpose

    Bimolecular Fluorescence Complimentation (BiFC) assay. Mammalian expression vector to generate stable, inducible cell line expressing N-terminally tagged HA-GFP1-10-actin.

    Depositor

    Maria Vartiainen

    Article

    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

    Insert

    actin beta (ACTB Human)
    

    Tags

    HA-GFP1-10

    Expression

    Mammalian

    Promoter

    CMV/TO

    Available Since

    May 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge