We narrowed to 24,925 results for: crispr
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Plasmid#192666Purpose3rd generation lenti vector encoding dCas9-VP64 with P2A MS2-VP64 and T2A Blast resistance markerDepositorInsertdCas9-VP64, MS2-VP64
UseCRISPR and LentiviralExpressionMammalianMutationD10A and N863A in Cas9PromoterEF1aAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
CAG-FNLS-T2A-EGFP-ires-puro
Plasmid#121169PurposeCAG promoter driven expression of FNLS BE3 base editor, EGFP and Puromycin resistance.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFNLS-T2A-EGFP-ires-puro
UseCRISPRTagsT2A-EGFP-ires-puroExpressionMammalianAvailable sinceJuly 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJAT21
Plasmid#204309PurposeFor generating targeting inversions. N-terminal split GFP, expressed in abdomen and mouthpartsDepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertie1-GFPn
UseCRISPRPromoterie1Available sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJAT25
Plasmid#204310PurposeFor generating targeting inversions. C-terminal split GFP, expressed in abdomen and mouthparts.DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertie1-GFPc
UseCRISPRPromoterie1Available sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJAT62
Plasmid#204301PurposeExpress phiC31 under control of D. melanoagaster vasa promoter, with Vasa 3’UTR.DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertie1-EGFP
UseCRISPRPromoterie1Available sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBLO5.01
Plasmid#121418PurposepUC19_BsaI-dCas9-BsaIDepositorInsertdcas9
ExpressionBacterialPromoterlacAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAH233
Plasmid#121144PurposeExpression of gRNA with BbsI placeholder for target cloningDepositorInsertgRNA cassette
UseCRISPRExpressionYeastPromoterS.pombe rrk1Available sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAC1818_pCR8-gRG6-SA
Plasmid#118652PurposeTransient transfection; Expressed RG6-SA CasRx gRNA; Gateway DonorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertgRG6-SA
UseCRISPRExpressionMammalianPromoterU6Available sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGNW6
Plasmid#122093Purposesuicide vector for integration into Pseudomonas genome after insertion of homologous regions at the multiple cloning site; successful integration causes green fluorescence in cellsDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGNW4
Plasmid#122088Purposesuicide vector for integration into Pseudomonas genome after insertion of homologous regions at the multiple cloning site; successful integration causes green fluorescence in cellsDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgRb1/Cre
Plasmid#89647PurposeExpresses an Rb1-targeting gRNA and Cre-recombinaseDepositorAvailable sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDF0352 HvsCas7-11 with C-term Nes mammalian expression
Plasmid#172516PurposeEncodes human codon optimized HvsCas7-11 protein with C-terminal NES tag for expression in mammalian cellsDepositorInsertC-term Nes HvsCas7-11 mammalian expression
ExpressionMammalianAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
R26-EGFP HMEJ donor vector
Plasmid#137927PurposeROSA26 knock-in vector with homology-mediated end joining(HMEJ)-based methodDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSA-EGFP-bpA with homology arms
UseMouse TargetingAvailable sinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHR-TRE3G-NLS-tdMCP-mCherry
Plasmid#99909PurposeFor the expression of NLS-tdMCP-mCherryDepositorInsertNLS-tdMCP-mCherry
UseLentiviralExpressionMammalianPromoterTRE3GAvailable sinceOct. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAct:dCas9-GCN4
Plasmid#78899PurposeExpresses dCas9-GCN4 under actin promoter for SunTag CRISPRa in Drosophila cell cultureDepositorInsertdCas9-10XGCN4
UseCRISPRTagsHAExpressionInsectPromoterpActin (Drosophila)Available sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMM733
Plasmid#68898PurposeIPTG-inducible CRISPRi vector targeting NanoLuc (NL4), constiutive NanoLuc, pNBU2 backbone, AmpRDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsdCas9
LacI
sgRNA
NanoLuc
Available sinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUBC_HA_2xPP7-VP64
Plasmid#68418PurposeTransient expression of two tandem copies of the non-aggregating delta-FG mutant, fused to the VP64 transcription activator, in mammalian cells, under a UBC promoterDepositorInsertPP7(deltaFG)
UseSynthetic BiologyTagsHA and VP64ExpressionMammalianMutationPP7: [delta]67_74 (non-aggregating)Promoterhuman UbCAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Solo-mStayGold-CENPA
Plasmid#227283PurposeDonor template for mStayGold insertion into the N-terminus of the CENPA locus. For centromere visualization. To be co-transfected with sgRNA plasmid px330-CENPA (Addgene #227282)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCENPA Homology Arms flanking a mStayGold Tag (CENPA Human)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-AAVS1-PGK-Puro-moxGFP
Plasmid#227275PurposeAAVS1 targeting donor for the insertion of Puro and a medium strength PGK expressing moxGFP. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAAVS1 Homology Arms flanking a 2A-Puro-PGK-moxGFP cassette (AAVS1 Human)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-mStayGold-EFS-Zeo
Plasmid#227258PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-EFS-Zeo cassetteDepositorTypeEmpty backboneUseCRISPR; Donor cassetteExpressionMammalianAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only