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20,066 results

Showing: 7461 - 7480 of 20066 results

  7. His-ZZ-TEV-SNAPf DHC1_IC2C_LIC2_Tctex1_Robl1_LC8

    Plasmid
    #111903
    Purpose
    Full length human cytoplasmic dynein 1 complex, optimised for Sf9 expression. 8xHis-ZZ tag followed by TEV cleavage site and SNAPf tag on the N-terminus of dynein heavy chain 1.
    Depositor
    Andrew Carter
    Inserts
    His-ZZ-LTLT-SNAPf-DYNC1H1 (DYNC1H1 Human)
    DYNC1I2 (DYNC1I2 Human)
    DYNC1LI2 (DYNC1LI2 Human)
    DYNLT1 (DYNLT1 Human)
    DYNLRB1 (DYNLRB1 Human)
    DYNLL1 (DYNLL1 Human)
    Tags
    8xHis, SNAPf-tag, TEV site, and ZZ-tag
    Expression
    Insect
    Mutation
    Optimised for expression in Sf9 cells.
    Available Since
    July 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    111903-plasmid-map-sequence-id-215307

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  10. U6-BbsI-DR_CMV-minidCas13X.1-REPAIRv2-BGHpA_CMV-EGFP-BGHpA

    Plasmid
    #171383
    Purpose
    Expression vector for encoding a human codon-optimized minidCas13X.1-REPAIRv2 driven by CMV promoter,EGFP driven by CMV promoter and U6-driven crRNAs cloning site.
    Depositor
    Hui Yang
    Article
    Xu et al Nat Methods. 2021 May;18(5):499-506. doi
    Insert
    humanized minidCas13X.1-REPAIRv2
    Tags
    HA
    Expression
    Mammalian
    Promoter
    CMV, U6
    Available Since
    July 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    171383-plasmid-map-sequence-id-337350

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  13. pRDA_221

    Plasmid
    #169142
    Purpose
    Constitutive expression of EGFP and sgRNAs targeted to EGFP for measurement of activity of AsCas12a\EnAsCas12a.
    Depositor
    John Doench, David Root
    Insert
    EGFP , EGFP sgRNA
    Use
    CRISPR and Lentiviral
    Available Since
    June 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    169142-plasmid-map-sequence-id-334957

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  16. pC0039-CMV-dPspCas13b-GS-ADAR2DD(E488Q)

    Plasmid
    #103849
    Purpose
    dPspCas13b-ADAR2DD(E488Q) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA.
    Depositor
    Feng Zhang
    Article
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Inserts
    PspCas13b
    huADAR2DD (ADARB1 Human)
    Use
    CRISPR
    Tags
    GS and HIV NES
    Expression
    Mammalian
    Mutation
    Changed histidne 133 to alanine and histidine 105…
    Promoter
    CMV
    Available Since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    103849-plasmid-map-sequence-id-199152

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  17. pHJ12: CadC-VHH-Caffeine (No linker)

    Plasmid
    #108244
    Purpose
    Membrane bound transcription activator CadC fusing VHH-Caffeine. Upon ligand binding, CadC-VHH-Caffeine can be dimerized and activate down stream reporter gene sfGFP expression
    Depositor
    Jerome Bonnet
    Article
    Chang et al ACS Synth Biol. 2017 Oct 30. doi: 10.102
    Insert
    Membrane bound transcription activator CadC fusing with VHH-Caffeine antibody without external linker
    Use
    Synthetic Biology
    Promoter
    pLacO1
    Available Since
    May 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    108244-plasmid-map-sequence-id-210116

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  20. phCMV-229E-D15

    Plasmid
    #188908
    Purpose
    express truncated 229E spike for pseudovirus production
    Depositor
    David Nemazee
    Insert
    229E spike (S 229E)
    Expression
    Mammalian
    Mutation
    c-terminal 15aa deletion
    Promoter
    CMV
    Available Since
    Aug. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    188908-plasmid-map-sequence-id-370665

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Showing: 7461 - 7480 of 20066 results