We narrowed to 7,581 results for: ef1a
-
Plasmid#162786PurposeThe extracellular domain of Angiotensin converting enzyme 2 (ACE2) expressionDepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
Tags10xHis, FLAG, and hTPA leaderExpressionMammalianPromoterCHO EEF1A1 (Translation Elongation Factor 1 Alpha…Available SinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLEX307_PDHA2S293A
Plasmid#115197PurposeLentiviral transduction and expression of PDHA2S293A into any mammalian cellDepositorAvailable SinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
DNMT3ACD-CRY2-EGFP
Plasmid#82556PurposeEncodes DMNT3 under LITE2.0 systemDepositorUseAAVTags2A, NLS(alpha-imp), and NLS-VP64ExpressionMammalianMutationCRY2PHR (N-terminal photolyase homology region do…PromoterEF-1alphaAvailable SinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
TET1CD-CRY2-EGFP
Plasmid#82555PurposeEncodes TET1 under LITE2.0 systemDepositorUseAAVTags2A, NLS(alpha-imp), and NLS-VP64ExpressionMammalianMutationCRY2PHR (N-terminal photolyase homology region do…PromoterEF-1alphaAvailable SinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
L4315 IL-10Ra CTEVp chain in PolyTX-mTagBFP2
Plasmid#244171PurposeConstitutive expression of a MESA CTEVp chain encoding (N to C): IL10RaSS-3xFLAG-IL10RaECD-IL10RaTMD-CTEVp(190K)-PRS(M)-VP64-ZF6aDepositorInsertMESA CTEVp chain with human IL-10Ra SS, ECD and TMD, CTEVp (190K), TEVp PRS (M in P1'), and synTF (VP64-ZF6) (IL10RA Synthetic, Human)
UseSynthetic BiologyTagsIL10Ra signal peptide - 3xFLAGExpressionMammalianMutationCTEVp_190KPromoterhEF1aAvailable SinceOct. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
L4804 VEGFR1 CTEVp chain in PolyTX-mTagBFP2
Plasmid#244174PurposeConstitutive expression of a MESA CTEVp chain encoding (N to C): VEGFR1SS-3xFLAG-VEGFR1ECD-VEGFR1TMD-CTEVp(190K)-PRS(M)-VP64-ZF6DepositorInsertMESA CTEVp chain with human VEGFR1 SS, ECD and TMD, CTEVp (190K), TEVp PRS (M in P1'), and synTF (VP64-ZF6) (FLT1 Synthetic, Human)
UseSynthetic BiologyTagsVEGFR1 signal peptide - 3xFLAGExpressionMammalianMutationCTEVp_190KPromoterhEF1aAvailable SinceOct. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
L4308 IL-10Rb NTEVp chain (human IgG VH SS) in PolyTX-mNeonGreen
Plasmid#244169PurposeConstitutive expression of a MESA NTEVp chain encoding (N to C): hIgGVHSS-3xFLAG-IL10RbECD-IL10RbTMD-NTEVp(75S)DepositorInsertMESA NTEVp chain with human IgG-VH SS, human IL-10Rb ECD and TMD, and NTEVp 75S mutant (IL10RB Synthetic, Human)
UseSynthetic BiologyTagshuman IgG VH signal peptide - 3xFLAGExpressionMammalianMutationNTEVp_75SPromoterhEF1aAvailable SinceOct. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
L4312 IL-10Rb NTEVp chain (human CD8a SS) in PolyTX-mNeonGreen
Plasmid#244170PurposeConstitutive expression of a MESA NTEVp chain encoding (N to C): hCD8aSS-3xFLAG-IL10RbECD-IL10RbTMD-NTEVp(75S)DepositorInsertMESA NTEVp chain with human CD8a SS, human IL-10Rb ECD and TMD, and NTEVp (75S) (IL10RB Synthetic, Human)
UseSynthetic BiologyTagshuman CD8a signal peptide 3xFLAGExpressionMammalianMutationNTEVp_75SPromoterhEF1aAvailable SinceOct. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
L4791 VEGFR2 NTEVp chain (75S NTEVp mutant) in PolyTX-mNeonGreen
Plasmid#244172PurposeConstitutive expression of a MESA NTEVp chain encoding (N to C): VEGFR2SS-3xFLAG-VEGFR2ECD-VEGFR2TMD-NTEVp(75S)DepositorInsertMESA NTEVp chain with human VEGFR2 SS, ECD and TMD, and NTEVp (75S) (KDR Synthetic, Human)
UseSynthetic BiologyTagsVEGFR2 signal peptide - 3xFLAGExpressionMammalianMutationNTEVp_75SPromoterhEF1aAvailable SinceOct. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJT256 Lenti pEF-dCas9-3XFLAG-N+F-T2A-tagBFP-P2A-Blast
Plasmid#187332PurposedCas9 effector fusion for manipulating transcriptionDepositorInsertdCas9-3XFLAG-N+F-T2A-tagBFP-P2A-Blast
UseCRISPR and LentiviralTags3XFLAGExpressionMammalianPromoterpEF1aAvailable SinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJT257 Lenti pEF-dCas9-3XFLAG-Z+F-T2A-tagBFP-P2A-Blast
Plasmid#187333PurposedCas9 effector fusion for manipulating transcriptionDepositorInsertdCas9-3XFLAG-Z+F+N-T2A-tagBFP-P2A-Blast
UseCRISPR and LentiviralTags3XFLAGExpressionMammalianPromoterpEF1aAvailable SinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJT329 Lenti pEF-rTetR(SE-G72P)-3XFLAG-N+Z+F-T2A-mCherry-BSD-WPRE
Plasmid#187339PurposerTetR effector fusion for manipulating transcriptionDepositorInsertrTetR(SE-G72P)-3XFLAG-N+Z+F-T2A-mCherry-BSD-WPRE
UseLentiviral and Synthetic BiologyTags3XFLAGExpressionMammalianPromoterpEF1aAvailable SinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJT255 Lenti pEF-dCas9-3XFLAG-N+Z-T2A-tagBFP-P2A-Blast
Plasmid#187331PurposedCas9 effector fusion for manipulating transcriptionDepositorInsertdCas9-3XFLAG-N+Z-T2A-tagBFP-P2A-Blast
UseCRISPR and LentiviralTags3XFLAGExpressionMammalianPromoterpEF1aAvailable SinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC-hU6-shBACH1_BFP
Plasmid#199217PurposeExpression plasmid with shRNAmiR targeting BACH1, with a BFP reporter on the backbone.DepositorAvailable SinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC_hU6-shRKIP_BFP
Plasmid#199216PurposeExpression plasmid with shRNAmiR targeting RKIP (PEBP1), with a BFP reporter on the backbone.DepositorAvailable SinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
Donor_ANKRD1_KO_Hygro
Plasmid#186668PurposeDonor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. eEF1a promoter, hygromycin and polyA sequence is inserted between two homology arms.DepositorInsertANKRD1 KO Hygromycin (ANKRD1 Human)
ExpressionMammalianAvailable SinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLEX307_PDHA2S291A
Plasmid#115196PurposeLentiviral transduction and expression of PDHA2S291A into any mammalian cellDepositorAvailable SinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEX307_PDHA2S291A/S293A
Plasmid#115198PurposeLentiviral transduction and expression of PDHA2S291A/S293A into any mammalian cellDepositorAvailable SinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
HA tagged SphI ELL2 M133, 138, 186ILeu
Plasmid#127277PurposeExpresses human ELL2 with M133, 138, 186ILeu to prevent internal Met initiation peptides and contains HA tag near C-terminusDepositorInsertELL2 with Met 133, 138, 186 to Ileu (ELL2 Human)
ExpressionMammalianMutationMet 133, 138, 186 to Ileu; HA inserted at SphI af…PromoterpEF1aAvailable SinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-shDnd1-No1
Plasmid#70058PurposeTRC lentiviral vector for shRNA against mouse Dnd1 #1DepositorAvailable SinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only