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pAAV-hSyn-ASAP3-WPRE

Plasmid

#132331

Purpose

expresses ASAP3(ASAP-family genetically encoded voltage indicator) in mammalian cells, gene delivered by pAAV-hSyn vector

Depositor

Michael Lin

Article

Villette et al Cell. 2019 Dec 12;179(7):1590-1608.e23.

Insert

ASAP3

Use

AAV

Expression

Mammalian

Promoter

hSynapsin

Available Since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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Integrin Beta1-mPA-GFP

Plasmid

#186302

Purpose

Expresses mPA-GFP tagged Integrin beta-1 in mammalian cells on a CMV promoter

Depositor

Pakorn Kanchanawong

Article

Jain et al Nat Protoc. 2022 Jul 27. pii: 10.1038/s4

Insert

Integrin Beta 1 (ITGB1 Human)

Tags

mPA-GFP

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pPICZc-ScAct1*-thymosinB-8His

Plasmid

#111148

Purpose

Expresses Saccharomyces cerevisiae Act1 (codon is optimised for expression in Pichia pastoris) fused with thymosin beta and a His tag. TH3-37

Depositor

Mohan Balasubramanian

Article

Hatano et al J Cell Sci. 2018 Mar 13. pii: jcs.213827

Insert

Act1 (ACT1 Budding Yeast)

Use

Pichia pastoris integration plasmid

Tags

Thymosin beta and a His-tag

Expression

Yeast

Promoter

AOX1

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pMXs-IRES-blast GFP-SPNS1

Plasmid

#198571

Purpose

SPNS1 codon-optimized orf with n-terminal 3xFlag GFP in pMXs-IRES-Blast vector

Depositor

Monther Abu-Remaileh

Article

Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

Insert

SPNS1 with n-terminal 3xFlag-GFP fusion (SPNS1 Human)

Use

Retroviral

Tags

3xFlag-GFP

Expression

Mammalian

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-AcGFP-Sec61-IRES-Blast

Plasmid

#154009

Purpose

Lentiviral vector plasmid for integration and constitutive expression of AcGFP fused to Sec61 in mammalian cells – gift from D. Gerlich (IMBA, Vienna)

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

acGFP-Sec61 (SEC61B Human)

Use

Lentiviral

Expression

Mammalian

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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Cav2.1 HA pcDNA3

Plasmid

#206069

Purpose

expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and an exofacial double HA tag in domain II

Depositor

Annette Dolphin

Article

Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

Insert

cacna1a (Cacna1a Rat)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pGMC00035 (aka pMG0784)

Plasmid

#210768

Purpose

Lentiviral vector to express human ADRM1

Depositor

Raj Chari

Article

Osei-Amponsa et al Mol Cell. 2024 Feb 1;84(3):522-537.e8. d

Insert

ADRM1 (ADRM1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-camk2-Sthk-p2a-bpac(WT) minWPRE

Plasmid

#118274

Purpose

Expresses the SthK channel & bPAC(WT)-mCherry under a minimal CamKII promoter

Depositor

Dietmar Schmitz

Article

Bernal Sierra et al Nat Commun. 2018 Nov 5;9(1):4611. doi: 1

Inserts

bPAC(WT)
SthK

Use

AAV

Tags

His tag, P2A, mCherry, and myc tag

Expression

Mammalian

Promoter

CamK II

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pCR8/GW/TOPO-ETV1

Plasmid

#98623

Purpose

Gateway entry TOPO TA cloning vector for ETV1

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

ETV1 (Etv1 Mouse)

Use

Topo ta cloning vector

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pCR8/GW/TOPO-ELK4

Plasmid

#98617

Purpose

Gateway entry TOPO TA cloning vector for ELK4

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

ELK4 (Elk4 Mouse)

Use

Topo ta cloning vector

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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