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pDONR223-FLJ25006

Plasmid

#23367

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

FLJ25006 (SGK494 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-FLJ23356

Plasmid

#23375

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

FLJ23356 (POMK Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-MAK

Plasmid

#23624

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAK (MAK Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pDONR223-ETNK1

Plasmid

#23634

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ETNK1 (ETNK1 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-CKMT2

Plasmid

#23746

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CKMT2 (CKMT2 Human)

Use

Gateway Cloning

Mutation

S6P

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pDONR223-IHPK2

Plasmid

#23799

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

IHPK2 (IP6K2 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDQM049

Plasmid

#247635

Purpose

to generate mRNA for membrane mStayGold(E138D) for zebrafish

Depositor

Gokul Upadhyayula

Publication

Tschanz et al MicroPubl Biol. 2025 Nov 25;2025:10.1791

Insert

2x Lyn Kinase membrane localization sequence

Use

Zebrafish mrna expression using sp6

Tags

mStayGold(E138D)

Available since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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Ubc-iRFP670-24xMS2-24xPP7

Plasmid

#238916

Purpose

Contains Ubc promoter expressing iRFP670 mRNA taged 24xMS2 motif and 24xPP7 motif

Depositor

Jian-Hui Jiang

Publication

Zhou et al Angew Chem Int Ed Engl. 2025 Apr 10:e202

Insert

iRFP670-24xMS2-24xPP7

Use

Lentiviral

Expression

Mammalian

Promoter

Ubc

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pTS2351_Tier3(SB)-Zeo

Plasmid

#169648

Purpose

Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a ZeoR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-ZeoR-pA-3'ITR)

Depositor

Martin Fussenegger

Publication

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PRPBSA-driven ZeoR expression

Expression

Mammalian

Promoter

PRPBSA

Available since

July 7, 2022

Availability

Academic Institutions and Nonprofits only

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huβB1cry:mAppleCAAX

Plasmid

#122451

Purpose

Human BB1 crystallin promoter driving mApple-CAAX in Tol2 destination vector

Depositor

Thomas Schilling

Publication

Vorontsova et al J Vis Exp. 2019 May 6;(147). doi: 10.379

Insert

Human BB1 crystallin:mApple_CAAX (CRYBB1 Human)

Expression

Bacterial

Promoter

300bp Human Beta B1 crystallin promoter

Available since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pFGL1344

Plasmid

#116902

Purpose

Alp6:Bar:mCherry (SPB/MTOC marker)

Depositor

Naweed Naqvi

Publication

Yadav et al MBio. 2019 Jul 30;10(4). pii: mBio.01581

Inserts

Alp6 (partial ORF)
Alp6 downstream region

Use

Fungal expression (in magnaporthe oryzae)

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pEAHISMRSA2676

Plasmid

#96970

Purpose

expression of methicillin-resistant Staphylococcus aureus orf 2676

Depositor

Huanting Liu, James Naismith

Publication

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

Sar2676

Tags

N-ter TEV protease cleavable 6His

Mutation

none

Promoter

T7

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISMRSAMVAK

Plasmid

#97000

Purpose

expression of methicillin-resistant Staphylococcus aureus MVAK protein

Depositor

Huanting Liu, James Naismith

Publication

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

Mevalonate kinase

Tags

N-ter TEV protease cleavable 6His

Mutation

none

Promoter

T7

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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TAL3184

Plasmid

#41358

Depositor

Keith Joung

Publication

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-stac-Left (LOC100005402 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only
TAL3185

Plasmid

#41359

Depositor

Keith Joung

Publication

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-stac-Right (LOC100005402 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only
pET-hisM5RT

Plasmid

#216672

Purpose

Expresses his-tagged Mut5 reverse transcriptase in E. coli hosts

Depositor

Po-Min Chiang

Publication

Hsueh et al PLoS Biol. 2025 Dec 15;23(12):e3003537.

Insert

thermostable M-MuLV reverse transcriptase variants

Tags

his-tag

Expression

Bacterial

Promoter

T7

Available since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pET-22b(+) pCopA RBS30 mscarlet

Plasmid

#226370

Purpose

Plasmid carries copper sensor circuit genes for whole-cell sensing of copper ions with RBS30

Depositor

Urartu Seker

Publication

Koksaldi et al Biosens Bioelectron. 2024 Aug 10;264:116

Insert

mScarlet

Tags

6x Histag

Expression

Bacterial

Promoter

copA promoter (pCopA)

Available since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-20nmEML-mRFP

Plasmid

#231178

Purpose

Stabilizes the ER-mitochondrial distance at 20 nm; tagged with mRFP

Depositor

Dmitry Lim

Publication

Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

20nmEML-mRFP

Expression

Mammalian

Promoter

CMV

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3.1-30nmEML-mRFP

Plasmid

#231179

Purpose

Stabilizes the ER-mitochondrial distance at 30 nm; tagged with mRFP

Depositor

Dmitry Lim

Publication

Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

30nmEML-mRFP

Expression

Mammalian

Promoter

CMV

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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RSRC1

Plasmid

#155983

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Publication

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

RSRC1 (RSRC1 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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