We narrowed to 17,860 results for: COMP

1. pEBTet-SNAP-Cep27

   Plasmid

   #136808

   Purpose

   Mammalian expression of the centrosomal protein Cep27 N-terminally fused to SNAP-tag

   Depositor

   Kai Johnsson

   Article

   Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

   Insert

   SNAP-Cep27 (HAUS2 Synthetic, Human)
   

   Use

   Tags

   FLAG-tag / His-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TetO2

   Available since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. Nsp13 deltaN1-EGFP

   Plasmid

   #165127

   Purpose

   mammalian expression and localization

   Depositor

   Bruno Antonny

   Article

   Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

   Insert

   Nsp13 deltaN1 (ORF1ab codon optimized SARS-COV-2)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Nsp13 deltaN2-EGFP

   Plasmid

   #165128

   Purpose

   mammalian expression and localization

   Depositor

   Bruno Antonny

   Article

   Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

   Insert

   Nsp13 deltaN2 (ORF1ab codon optimized SARS-COV-2)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Nsp14D-EGFP

   Plasmid

   #165125

   Purpose

   mammalian expression and localization

   Depositor

   Bruno Antonny

   Article

   Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

   Insert

   Nsp14D (ORF1ab codon optimized SARS-COV-2)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDF-casBCDE(g8)

   Plasmid

   #89727

   Purpose

   Expresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing 33-nt spacer (referred to as 'wt') matching a protospacer in the M13 phage genome.

   Depositor

   Konstantin Kuznedelov, Konstantin Severinov

   Article

   Kuznedelov et al Nucleic Acids Res. 2016 Dec 15;44(22):10

   Insert

   CRISPR array
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. 3xNLS-NLP-cMyc-cMyc AspCas12a

   Plasmid

   #182122

   Purpose

   pET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc AspCas12a in bacteria

   Depositor

   Scot Wolfe

   Article

   Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

   Insert

   3xNLS-NLP-cMyc-cMyc AspCas12a
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Epac-S-H96

   Plasmid

   #170342

   Purpose

   CFP(UST)Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

   Depositor

   Kees Jalink

   Article

   van der Krogt et al PLoS ONE. 2008 . 3(4):e1916.

   Insert

   CFP(UST)Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) (RAPGEF3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Deletion of DEP domain, Catalytically dead T781A …

   Promoter

   CMV

   Available since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. deltaVP1 + VP2C-GFP

   Plasmid

   #166676

   Purpose

   Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and GFP tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3)

   Depositor

   Claudia Vickers

   Article

   Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

   Inserts

   VP2C-GFP
   Murine polyomavirus deltaVP1 (NLS deletion mutant)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1 and GAL10

   Available since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1(+)-RIα(D/D+linker)-EGFP

   Plasmid

   #181842

   Purpose

   EGFP-tagged docking/dimerization (D/D) and linker region of PKA RIα; for mammalian expression.

   Depositor

   Jin Zhang

   Article

   Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

   Insert

   RIα-(D/D+linker)EGFP (PRKAR1A Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Contains only residues 12-112 of PKA RIα.

   Promoter

   CMV

   Available since

   June 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDEST27-GST-ATXN1 FL(30Q)-S776D

    Plasmid

    #21755

    Depositor

    Huda Zoghbi

    Article

    Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

    Insert

    Ataxin-1 (ATXN1 Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    insert contains a stretch of 32 amino acids (30 Q…

    Promoter

    CMV

    Available since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pMCh2007_pLenti_Syn_mChe-cdc42E7

    Plasmid

    #118620

    Purpose

    expression of mChe-tagged cdc42E7 under pSyn promoter for primary neurons

    Depositor

    Marina Chekulaeva

    Article

    Ciolli Mattioli et al Nucleic Acids Res. 2018 Dec 22. pii: 525

    Insert

    cdc42E7 (Cdc42 Mouse)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    changed cagtctg to ggcataa (667 - 673 nt in 3…

    Promoter

    synapsin I (rat)

    Available since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHH0103_ITSN1-2/5

    Plasmid

    #91385

    Purpose

    Protein expression and purification of human SH3 domain construct ITSN1-2/5

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    ITSN1-2/5 (ITSN1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28a-PTBP1-RRM12CCtoSS

    Plasmid

    #89155

    Purpose

    Recombinant expression of PTBP1-RRM12 C250S,C251S in E.coli

    Depositor

    Frédéric Allain

    Article

    Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n

    Insert

    Polypyrimidine Tract Binding Protein 1 RNA Recognition Motif 1+2 mutant C250S, C251S (PTBP1 Human)
    

    Use

    Tags

    hexa His

    Expression

    Bacterial

    Mutation

    mutations: Cys 250 to Ser, Cys 251 to Ser

    Promoter

    T7

    Available since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHH0103_LASP1-1/1-SV1/2

    Plasmid

    #91399

    Purpose

    Protein expression and purification of human SH3 domain construct LASP1-1/1-SV1/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    LASP1-1/1-SV1/2 (LASP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CRAF_S29A_pcDNA3.1

    Plasmid

    #86506

    Purpose

    Expresses CRAF_S29A in mammalian cells [Edit]

    Depositor

    Dustin Maly

    Article

    Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

    Insert

    CRAF (RAF1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S29A

    Promoter

    CMV

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. MO70-N-Flag-hCRACR2A-isoform-a-Q604L

    Plasmid

    #79595

    Purpose

    Transient and retroviral expression

    Depositor

    Yousang Gwack

    Article

    Srikanth et al Sci Signal. 2016 Mar 22;9(420):ra31. doi

    Insert

    CRACR2A-isoform-a (CRACR2A Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Q604L

    Promoter

    CMV

    Available since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

17. MO70-N-Flag-hCRACR2A-isoform-a-T559N

    Plasmid

    #79594

    Purpose

    Transient and retroviral expression

    Depositor

    Yousang Gwack

    Article

    Srikanth et al Sci Signal. 2016 Mar 22;9(420):ra31. doi

    Insert

    CRACR2A-isoform-a (CRACR2A Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    T559N

    Promoter

    CMV

    Available since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pHH0103_GRB2-2/2

    Plasmid

    #91410

    Purpose

    Protein expression and purification of human SH3 domain construct GRB2-2/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    GRB2-2/2 (GRB2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHH0103_GRAP2-2/2

    Plasmid

    #91368

    Purpose

    Protein expression and purification of human SH3 domain construct GRAP2-2/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    GRAP2-2/2 (GRAP2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHH0103_CRK-2/2-SV2/2

    Plasmid

    #91343

    Purpose

    Protein expression and purification of human SH3 domain construct CRK-2/2-SV2/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    CRK-2/2-SV2/2 (CRK Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pIRES:mTREK-1(K271Q)

    Plasmid

    #133273

    Purpose

    Mammalian expression vector. It will generate the mouse TREK-1 channel full lenght K271Q mutant fused to a N-terminal HA tag

    Depositor

    Dan Minor

    Article

    Lolicato et al Nature. 2017 Jul 20;547(7663):364-368. d

    Insert

    Potassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian and Yeast

    Mutation

    K271Q

    Promoter

    Available since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pHH0103_EPS8L2-1/1

    Plasmid

    #91526

    Purpose

    Protein expression and purification of human SH3 domain construct EPS8L2-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    EPS8L2-1/1 (EPS8L2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. p28BIOHTEV-LIC: nsp16_SARS-CoV-2|nsp10_SARS-CoV-2

    Plasmid

    #167848

    Purpose

    Bacterial expression of the SARS-CoV-2 proteins nsp10 and nsp16 from a bicistronic mRNA

    Depositor

    Cheryl Arrowsmith

    Article

    Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

    Insert

    nsp10, nsp16
    

    Use

    Tags

    6xHis and AviTag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 22, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. pfast bac cry1myc

    Plasmid

    #51892

    Purpose

    Backbone plasmid is pfastBacHTa from invitrogen

    Depositor

    Joseph Takahashi

    Article

    Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

    Insert

    cry1 (Cry1 Mouse)
    

    Use

    Tags

    3x Flag and myc

    Expression

    Mutation

    Promoter

    polyhedrin promoter

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

25. pHH0103_SRC-1/1-IS1/2

    Plasmid

    #91349

    Purpose

    Protein expression and purification of human SH3 domain construct SRC-1/1-IS1/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    SRC-1/1-IS1/2 (SRC Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGEX-6P-1 p37 SHPmut

    Plasmid

    #113503

    Purpose

    Bacterial expression of GST-tagged p37 that is p97 binding deficient due to mutations in the SHP box

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    p37 (Ubxn2b Mouse)
    

    Use

    Tags

    GST-PreScission protease site

    Expression

    Bacterial

    Mutation

    F215A/E218A/QKL220-222AAA

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pTKan-p35S::Csy4-pNOS::cogRFP (C134, JBEI-15901)

    Plasmid

    #110146

    Purpose

    Transformation and expression of Csy4 and cogDsRed proteins in plants

    Depositor

    Dominique Loque

    Article

    Liang et al ACS Synth Biol. 2017 May 19;6(5):806-816

    Insert

    Csy4 (ATCS Mustard Weed)
    

    Use

    Tags

    cogRFP

    Expression

    Bacterial and Plant

    Mutation

    Promoter

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEBTetBl-CLIP-Cep70

    Plasmid

    #136870

    Purpose

    Mammalian expression of the centrosomal protein Cep70 N-terminally fused to CLIP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    CLIP-Cep70 (CEP70 Synthetic, Human)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pHH0103_ASAP1-1/1

    Plasmid

    #91501

    Purpose

    Protein expression and purification of human SH3 domain construct ASAP1-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    ASAP1-1/1 (ASAP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEBTetDBl-CLIP-Cep70

    Plasmid

    #136871

    Purpose

    Mammalian expression of the centrosomal protein Cep70 N-terminally fused to CLIP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    CLIP-Cep70 (CEP70 Synthetic, Human)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge