Addgene

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Showing: 7521 - 7540 of 56584 results

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LgBit-NbV5

Plasmid

#201476

Purpose

NanoBit detection of V5-tagged protein

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

LgBit-NbV5

Use

Tags

LgBit

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.TBG mCherry-LDTS-eLIR

Plasmid

#189002

Purpose

lipophagy-inducing adaptor protein, AAV

Depositor

Atsushi Hoshino

Article

Minami et al Nat Commun. 2023 Jul 13;14(1):4084. doi:

Insert

LDTS-eLIR, mCherry

Use

AAV

Tags

HA

Expression

Mutation

Promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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WNK2 WT

Plasmid

#24569

Depositor

Joseph Costello

Article

Hong et al Proc Natl Acad Sci U S A. 2007 Jun 26. 1

Insert

WNK2 (WNK2 Human)

Use

Tags

His6 and V5

Expression

Mammalian

Mutation

Promoter

Available since

May 20, 2010

Availability

Academic Institutions and Nonprofits only

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pT2ADWpuro_2paRAF

Plasmid

#129653

Purpose

For generating cell line expressing the 2P-activatable RAF system with P2A and full-length RAF1

Depositor

Michiyuki Matsuda

Article

Kinjo et al Nat Methods. 2019 Sep 9. pii: 10.1038/s4

Insert

2paCRY2-Raf1-2A-CIBN-mScarlet-KRasCAAX

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-hAgo2

Plasmid

#11590

Depositor

Edward Chan

Article

Jakymiw et al Nat Cell Biol. 2005 Dec . 7(12):1267-74.

Insert

Ago2 (AGO2 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2006

Availability

Academic Institutions and Nonprofits only

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pMP71-Flt3L

Plasmid

#174600

Purpose

Secretes the extracellular domain of murine Flt3L

Depositor

Stanley Riddell

Article

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Insert

secretes extracellular domain of mouse Flt3-L

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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NbAlfa-LgBit

Plasmid

#201483

Purpose

NanoBit detection of ALFA-tagged protein

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

NbALFA-LgBit

Use

Tags

LgBit

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pQCasTns(Ptr)-array 8

Plasmid

#175580

Purpose

Expression of CRISPR-associated transposases

Depositor

Sheng Yang

Article

Yang et al Nucleic Acids Res. 2021 Sep 3. pii: 6363

Insert

PtrTniQ, PtrCas5/8, PtrCas7, PtrCas6, PtrTnsABC, CRISPR(8 CRISPR array)

Use

Tags

Expression

Bacterial

Mutation

NO

Promoter

Available since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-FLAG-LOV*-MVP

Plasmid

#202206

Purpose

Expresses the photoproximity labeling catalyst LOV* fused to MVP in mammalian cells

Depositor

Thomas Muir

Article

Hananya et al Proc Natl Acad Sci U S A. 2023 Apr 18;12

Insert

Major vault protein (MVP Human)

Use

Tags

FLAG and LOV*

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA3-TSC1

Plasmid

#19911

Depositor

Yue Xiong

Article

Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

Insert

Tsc1 (Tuberous sclerosis 1) (TSC1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

April 1, 2009

Availability

Academic Institutions and Nonprofits only

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pTRIPZ-shNTC

Plasmid

#217638

Purpose

Dox inducible shRNA non targeting control shRNA

Depositor

Constanze Bonifer

Article

Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

Insert

shNTC

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCBI-LexA-CIB1-biLINuS

Plasmid

#192605

Purpose

Plasmid for constitutive expression of LexA-CIB1-biLINuS.

Depositor

Yingxiao Wang

Article

Huang et al Sci Adv. 2020 Feb 19;6(8):eaay9209. doi:

Insert

LexA-CIB1-biLINuS

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

pCAGGS

Available since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pFastBac-Dual- GST-TEV-TBK1

Plasmid

#208875

Purpose

For expression in insect cells of TBK1

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

TBK1 (TBK1 Human)

Use

Tags

GST-TEV

Expression

Insect

Mutation

Promoter

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pRR-ERbeta-5Z

Plasmid

#23062

Depositor

Charles Miller

Article

Miller et al Plasmid. 2009 Dec 3. ():.

Insert

ERbeta (ESR2 Human)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 27, 2010

Availability

Academic Institutions and Nonprofits only
pMP71-IFN-B

Plasmid

#174597

Purpose

Expresses secreted murine interferon beta

Depositor

Stanley Riddell

Article

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Insert

secreted IFN-beta

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pCBI-CRY2PHR-VPR

Plasmid

#192606

Purpose

Plasmid for constitutive expression of CRY2PHR-VPR.

Depositor

Yingxiao Wang

Article

Huang et al Sci Adv. 2020 Feb 19;6(8):eaay9209. doi:

Insert

CRY2PHR-VPR

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pRK1035

Plasmid

#8832

Depositor

David Waugh

Article

Nallamsetty et al Protein Expr Purif. 2004 Nov . 38(1):108

Insert

TVMV protease

Use

Tags

His and MBP (cleaved)

Expression

Bacterial

Mutation

synthetic gene with codons optimized for E. coli

Promoter

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-nls-st1dCas9-3nls-3XGFP-nls

Plasmid

#64112

Purpose

Expression of St1 dCas9-3XGFP in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

St1 dCas9

Use

CRISPR and Lentiviral

Tags

3XsfGFP

Expression

Mammalian

Mutation

Promoter

CMV-TO

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pSK-mU6-Cj-sgRNA-SV40-puro

Plasmid

#192128

Purpose

Expresses Cj-SgRNA scaffold cloned with BbsI and puromycin resistance gene

Depositor

Yongming Wang

Article

Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

Insert

Cj-SgRNA scaffold

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-CLCa

Plasmid

#196912

Purpose

Visualization of clathrin-coated pit formation in living mammalian cells

Depositor

Kenji Sugimoto

Article

Sakaushi et al Biosci Biotechnol Biochem. 2007 Feb;71(2

Insert

Clathrin-Coated light chain

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

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